PennState/Project/Dosimeter/InitialTest

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|[[PennState|Home]]
 
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|[[PennState/Project/Diauxie|Diauxie Project]]
 
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|[[PennState/Project/Dosimeter|Dosimeter Project]]
 
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|[[PennState/People|People]]
 
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|[[PennState/Lab|Lab]]
 
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'''Dosimeter Test Construct'''
'''Dosimeter Test Construct'''
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To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluoresence plate reading
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To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluorescence plate reading
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This construct uses the lambda operator to express GFP when induced by radiation.
This construct uses the lambda operator to express GFP when induced by radiation.
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However, to establish the "off" state which corresponds to the lysogenic state of the WT lambda phage, it is necessary to use a second plasmid that produces LVA tagged Cl upon IPTG induction. Additionally, the plasmid harboring this sequence is IPTG inducible.
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<html><img src="http://openwetware.org/images/d/d0/PSU_GFL_IGEM_Establishing_Plasmid.jpg" id="logo" name="logo" height="128" width="332" alt="" /><br></html>
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This construct uses the pLAC operator to produce LVA tagged Cl upon IPTG induction.
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Latest revision as of 03:20, 27 October 2007

Dosimeter Test Construct

To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluorescence plate reading



This construct uses the lambda operator to express GFP when induced by radiation.

However, to establish the "off" state which corresponds to the lysogenic state of the WT lambda phage, it is necessary to use a second plasmid that produces LVA tagged Cl upon IPTG induction. Additionally, the plasmid harboring this sequence is IPTG inducible.



This construct uses the pLAC operator to produce LVA tagged Cl upon IPTG induction.