Primers Design

From 2007.igem.org

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We have design two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:
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We designed two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:
                         [[Image:oligo.jpg]]
                         [[Image:oligo.jpg]]
2X ORE – CYC1 Hind III ORE Fw
2X ORE – CYC1 Hind III ORE Fw
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5’-CCC [[AAGCTT]] TAGTCAATAATGGGAGCTGG-3’
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5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’
Tm 62°C
Tm 62°C
2X ORE – CYC1 BamHI Rev
2X ORE – CYC1 BamHI Rev
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5’-CGC [[GGATCC]] GTGCGTTTGTGGCGCCCTTA-3’
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5’-CGC GGATCC  GTGCGTTTGTGGCGCCCTTA-3’
Tm 68°C                                                                                     
Tm 68°C                                                                                     
1X ORE – CYC1 Hind III ORE Fw
1X ORE – CYC1 Hind III ORE Fw
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5’-CCC [[AAGCTT]] GGCTCTACCGAGCTGGTAGA-3’
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5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’
1X ORE – CYC1 BamHI Rev
1X ORE – CYC1 BamHI Rev
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5’-CGC [[GGATCC]] GTGCGTTTGTGGCGCCCTTA-3’
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5’-CGC GGATCC  GTGCGTTTGTGGCGCCCTTA-3’
LUCIFERASE  BamH1 Fv   
LUCIFERASE  BamH1 Fv   
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5’- CGC [[GGATCC]] ATGGAAGACGCCAAAAACATAA-3’       
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5’- CGC GGATCC  ATGGAAGACGCCAAAAACATAA-3’       
Tm 60°C
Tm 60°C
LUCIFERASE  SacI  Rv   
LUCIFERASE  SacI  Rv   
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5’- CGC [[GAGCTC]] TTAAAGCTTCTTTCCGCCCTT-3’
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5’- CGC GAGCTC  TTAAAGCTTCTTTCCGCCCTT-3’
Tm 60°C
Tm 60°C
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 +
PHO 80 fw Bam HI
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5’- CGC GGATCC ATGGAAAGCACATCAGGAGA-3’
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Tm 69.5°C
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 +
PHO 80 rev Sac I
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5’- CGC GAGCTC  TTAATCTGGCTTTGATCGCT-3’
 +
Tm 68.1°C

Latest revision as of 11:41, 23 October 2007

We designed two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:

                       Oligo.jpg

2X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’ Tm 62°C

2X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ Tm 68°C

1X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’

1X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’

LUCIFERASE BamH1 Fv 5’- CGC GGATCC ATGGAAGACGCCAAAAACATAA-3’ Tm 60°C

LUCIFERASE SacI Rv 5’- CGC GAGCTC TTAAAGCTTCTTTCCGCCCTT-3’ Tm 60°C

PHO 80 fw Bam HI 5’- CGC GGATCC ATGGAAAGCACATCAGGAGA-3’ Tm 69.5°C

PHO 80 rev Sac I 5’- CGC GAGCTC TTAATCTGGCTTTGATCGCT-3’ Tm 68.1°C