Primers Design

From 2007.igem.org

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Revision as of 09:43, 10 September 2007

We have design two oligo primers for 2Xore,1ORE and Luc with our restriction enzyme to amplify from plasmid p2UG-2XOre-Luc:

2X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT TAGTCAATAATGGGAGCTGG-3’ Tm 62°C

2X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’ Tm 68°C Oligo.jpg

1X ORE – CYC1 Hind III ORE Fw 5’-CCC AAGCTT GGCTCTACCGAGCTGGTAGA-3’

1X ORE – CYC1 BamHI Rev 5’-CGC GGATCC GTGCGTTTGTGGCGCCCTTA-3’

LUCIFERASE BamH1 Fv 5’- CGC GGATCC ATGGAAGACGCCAAAAACATAA-3’ Tm 60°C

LUCIFERASE SacI Rv 5’- CGC GAGCTC TTAAAGCTTCTTTCCGCCCTT-3’ Tm 60°C