Princeton

From 2007.igem.org

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[[Image:PrincetonLogo.png|750px| 2006 logo]]
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[[Image:GroupPic2007cropped.jpg|750px|Group picture]]
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<center>[[Image:PrincetonLogo2007.png|400x400px| 2007 logo]] [[Image:GroupPictureSmallcropped.jpg|750x400px|Group picture]]<br><br>
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<center><h3>Welcome</h3></center>
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=[[Princeton/Project Description | An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and Destruction]]=
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Welcome to Princeton's 2007 '''I'''nternational '''G'''enetically '''E'''ngineered '''M'''achines (iGEM) team wiki.  [http://www.princeton.edu '''Princeton'''] is one of almost sixty schools from around the world participating in creating synthetic genetic "machines" via the [https://2006.igem.org/wiki/index.php/Main_Page '''iGEM'''] competition. This is our fourth year in the iGEM program.  Our hope is to make a significant contribution to the community at large and have some fun along the way.
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<center><h3>  TEAM PRINCETON'S 2006 PROJECT SUMMARY</h3></center>
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The [[Princeton/People | Princeton University iGEM 2007 team]], consisting of 10 undergraduate students, 2 high school students, and 7 instructors, envisions a paradigm shift in the way one can target cancer and destroy the resulting cancerous cells.
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<center>Click [[Princeton:Project Summary|here for a summary of Princeton's iGEM2006 project]].
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Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. We have constructed many components of our system and are in the process of testing our plasmids in a proof of concept manner. Where possible, we will revise and improve upon our components based on mathematical modeling and experimental results.
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[[Image:heart-brain-brighter.jpg|250px|Brain and Heart]]</center>
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Please see the [[Princeton/overview | extended overview]] for more information.
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<h3>Team Members</h3>
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<center><h3>Team members</h3></center>
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*[[user:asichel|Andrew Sichel]]   
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*[[user:aswamina|Aparna Swaminathan]]
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*[[user:aatreya|Anand Atreya]]   
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*[[user:btsui|Bambi Tsui]]
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*[[user:bgeer|Bashir Geer]]
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*[[user:cdehart|Caroline DeHart]]
*[[user:cdehart|Caroline DeHart]]
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*[[user:dcooper|Devin Cooper]]
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*[[user:jmonk|Jon Monk]]
*[[user:jchiu|Justine Chiu]]
*[[user:jchiu|Justine Chiu]]
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*[[user:jmangual|John Mangual]]
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*[[user:katias|Katia Sherman]]
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*[[user:loughlin|Robert Loughlin]]
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*[[user:kmangal|Kush Mangal]]
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*[[user:okhowash|Oly Khowash]]
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*[[user:nyuan|Neal Yuan]]
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*[[user:rkamal|Ritu Kamal]]
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*[[user:pcowgill|Paul Cowgill]]
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*[[user:sgoli|Sanjiv Goli]]
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*[[user:vichij|Vichi Jagannathan]]
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*[[user:smomen|Safiyy Momen]]
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*[[user:sriutta|Stephen Riutta]]
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*[[user:tedtlee|Ted Lee]]
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*[[user:weiho|Wei Ho]]
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*[[user:asichel|Andrew Sichel]]
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<center><h3>Instructors</h3></center>
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*[[user:rweiss|Ron Weiss]] [http://www.princeton.edu/~rweiss Website]
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<h3>Instructors</h3>
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*[[user:ilemischka|Ihor Lemischka]] [http://www.molbio.princeton.edu/research_facultymember.php?id=25 Website]
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*[[user:Cil|Cil Purnick 2007]] [mailto:ppurnick@princeton.edu Contact Cil]
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*[[user:cry707|Christoph Schaniel]]
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*[[user:firsing|Steve Firsing]]
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*[[user:]]
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*[[user:rweiss|Ron Weiss]] [mailto:rweiss@princeton.edu Contact Ron] [http://www.princeton.edu/~rweiss Website]
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*[[user:Cil|Cil Purnick]] [mailto:ppurnick@princeton.edu Contact Cil]
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*[[user:ilemisch|Ihor Lemischka]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43268&deptid=92 Website]
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*[[user:cry707|Christoph Schaniel]] [http://directory.mssm.edu/faculty/facultyInfo.php?id=43270&deptid=92 Website]
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*[[user:pguye|Patrick Guye]]
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*[[user:eskamoah|Noah Davidsohn]]
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*[[user:saurabhg|Saurabh Gupta]]
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<center><h3>[[Princeton:Week One Agenda | Week 1 Agenda]]</h3>
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<h3>Resources</h3>
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<h3>[[Princeton:Week Two Agenda | Week 2 Agenda]]</h3>
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[[Princeton/literature | Literature on RNAi and Cancer]]
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<h3>[[Princeton:Week Three Agenda | Week 3 Agenda]]</h3>
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<h3>[[Princeton:Week Four Agenda | Week 4 Agenda]]</h3>
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<h3>[[Princeton:Week Five Agenda | Week 5 Agenda]]</h3>
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<h3>[[Princeton:Week Six Agenda  | Week 6 Agenda]]</h3>
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<h3>[[Princeton:Week Seven Agenda | Week 7 Agenda]]</h3>
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<h3>[[Princeton:Group Calendar|Group Calendar]]</h3>
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<h3>Pictures</h3>
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[[Princeton/People | Our happy team]]
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<h3>Research</h3></center>
 
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<h3>Overview</h3>
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[[Princeton/overview | Our Project]]
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<h3>Lab Work</h3>
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[[Princeton/lab | Lab work]]
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<center><h3>Resources</h3></center>
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<h3>Research</h3>
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[[Princeton/lab/experimentation | Experimentation]]
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*[https://static.igem.org/mediawiki/2006/8/8a/Igem_may_newsletter_%28letter_format%29.pdf May iGEM Newsletter]
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<h3>Bioinformatics</h3>
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*[[Princeton:Photos | Photo Journal]]
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[[Princeton/lab/bioinformatics | Bioinformatics]]
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*[[Readings | Other readings for the group]]
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*Flow chart
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*[http://syntheticbiology.org  Synthetic Biology]
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*Other labs of interest
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*Protocol sites
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<h3>Simulations</h3>
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[[Princeton/lab/simulation | Simulations]]
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<center><h3>Old Announcements</h3></center>
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[[Image:Example.jpg]]
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Latest revision as of 03:48, 27 October 2007


2007 logo Group picture

An RNAi-Enhanced Logic Circuit: Cancer-Specific Detection and Destruction

The Princeton University iGEM 2007 team, consisting of 10 undergraduate students, 2 high school students, and 7 instructors, envisions a paradigm shift in the way one can target cancer and destroy the resulting cancerous cells.

Using standard engineering practices, we have designed, and are in the process of implementing, a novel system that utilizes RNA interference (RNAi) to detect and destroy cancer cells in a tissue-specific manner. We are interfacing RNAi components together with promoter and repressor elements to form logic circuits, which allow the use of multiple criteria to target cancerous cells in a unique and highly specific manner. As a measure of safety, we utilize mutant lentiviral integrase to deliver our construct into the cell as a non-integrated plasmid, preventing any disruptive effects that could be attributed to pseudorandom integration by the lentivirus. This also ensures that the daughter cells will not inherit the cancer-detecting circuitry, thus preventing unintended proliferation of our construct and allow for more extensive and comprehensive cancer treatments. We have constructed many components of our system and are in the process of testing our plasmids in a proof of concept manner. Where possible, we will revise and improve upon our components based on mathematical modeling and experimental results.

Please see the extended overview for more information.

Team Members

Instructors

Resources

Literature on RNAi and Cancer

Pictures

Our happy team

Overview

Our Project

Lab Work

Lab work

Research

Experimentation

Bioinformatics

Bioinformatics

Simulations

Simulations