From 2007.igem.org

Bioinformatics

Biomarkers

Using microarray data provided by [http://www.cs.princeton.edu/~mhibbs/ Matthew Hibbs] of the [http://function.princeton.edu/ Function Group] at the [http://genomics.princeton.edu/ Lewis-Sigler Institute for Integrative Genomics], [http://www.princeton.edu/ Princeton University], it is noted that several factors, including GATA3, NPY1R, TFF1, and ALDH1A1, are likely overexpressed across 14000 microarray conditions in MCF-7 cells. In particular, GATA3 in MCF-7 is likely overexpressed in 67 of 70 microarrays.

The overexpressed factors may be used in conjunction in the AND circuit to provide greater specificity or the AND OR circuit to provide greater sensitivity.

Figure 1. image: Expression distribution of GATA3 in MCF-7 cells; microarray data from Matthew Hibbs.

Figure 2. image: Expression distribution of TFF1 in MCF-7 cells; microarray data from Matthew Hibbs.

Figure 3. image: Expression distribution of NPY1R in MCF-7 cells; microarray data from Matthew Hibbs.

Figure 4. image: Expression distribution of ALDH1A in MCF-7 cells; microarray data from Matthew Hibbs.