Samantha Liang Notebook

(Difference between revisions)

Revision as of 19:29, 4 June 2007

Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

I716201 is Bca1106A.Bca1112

       I716202 is Bca1106B.Bca1112 

       I716203 is Bca1128A.Bca1112 

       I716204 is Bca1090.Bca1112

Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)

I716205 is Bca1114.Bca1124

      I716206 is Bca1114.Bca1092

eek so many construction files and ape files to make.

to do

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 [[Template:BerkiGEM2007_SamanthaBiobricks | My Biobricks]]<br>
 ----
-==~~!~~==
+==--[[User:Samanthaliang|Samanthaliang]] 15:29, 4 June 2007 (EDT)==
-notes
+*Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?
+'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>
+*Made [rbs][ATG] variants with different ribosome binding sites
+''        I716201 is Bca1106A.Bca1112 <br>
+        I716202 is Bca1106B.Bca1112 <br>
+        I716203 is Bca1128A.Bca1112 <br>
+        I716204 is Bca1090.Bca1112 <br>''
+*Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
+''     I716205 is Bca1114.Bca1124 <br>
+       I716206 is Bca1114.Bca1092 <br>''
+*Tomorrow will make [Lox][Term][Lox] part
+*Then will put the rbsATG together with LoxTermLox
+eek so many construction files and ape files to make.
 === ===
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 to do
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