Samantha Liang Notebook

From 2007.igem.org

(Difference between revisions)
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'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>
'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>
*Made [rbs][ATG] variants with different ribosome binding sites
*Made [rbs][ATG] variants with different ribosome binding sites
-
''       I716201 is Bca1106A.Bca1112 <br>
+
      ''I716201 is Bca1106A.Bca1112'' <br>
-
         I716202 is Bca1106B.Bca1112 <br>
+
         ''I716202 is Bca1106B.Bca1112'' <br>
-
        I716203 is Bca1128A.Bca1112 <br>
+
      '' I716203 is Bca1128A.Bca1112'' <br>
-
         I716204 is Bca1090.Bca1112 <br>''
+
         ''I716204 is Bca1090.Bca1112'' <br>
*Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
*Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
-
''    I716205 is Bca1114.Bca1124 <br>
+
''    I716205 is Bca1114.Bca1124'' <br>
-
      I716206 is Bca1114.Bca1092 <br>''
+
      '' I716206 is Bca1114.Bca1092 ''<br>
*Tomorrow will make [Lox][Term][Lox] part
*Tomorrow will make [Lox][Term][Lox] part

Revision as of 19:29, 4 June 2007

My Construction Files
My Sequencing Files
My Biobricks


--Samanthaliang 15:29, 4 June 2007 (EDT)

  • Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

  • Made [rbs][ATG] variants with different ribosome binding sites
     I716201 is Bca1106A.Bca1112 
I716202 is Bca1106B.Bca1112
I716203 is Bca1128A.Bca1112
I716204 is Bca1090.Bca1112
  • Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)

I716205 is Bca1114.Bca1124

      I716206 is Bca1114.Bca1092 
  • Tomorrow will make [Lox][Term][Lox] part
  • Then will put the rbsATG together with LoxTermLox

eek so many construction files and ape files to make.


to do