Samantha Liang Notebook

From 2007.igem.org

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'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>
'''For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]''' <br>
*Made [rbs][ATG] variants with different ribosome binding sites
*Made [rbs][ATG] variants with different ribosome binding sites
-
      ''I716201 is Bca1106A.Bca1112'' <br>
+
        ''I716201 is Bca1106A.Bca1112'' <br>
         ''I716202 is Bca1106B.Bca1112'' <br>
         ''I716202 is Bca1106B.Bca1112'' <br>
-
      '' I716203 is Bca1128A.Bca1112'' <br>
+
        ''I716203 is Bca1128A.Bca1112'' <br>
         ''I716204 is Bca1090.Bca1112'' <br>
         ''I716204 is Bca1090.Bca1112'' <br>
*Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
*Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
-
''     I716205 is Bca1114.Bca1124'' <br>
+
      ''I716205 is Bca1114.Bca1124'' <br>
       '' I716206 is Bca1114.Bca1092 ''<br>
       '' I716206 is Bca1114.Bca1092 ''<br>

Revision as of 19:30, 4 June 2007

My Construction Files
My Sequencing Files
My Biobricks


--Samanthaliang 15:29, 4 June 2007 (EDT)

  • Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

  • Made [rbs][ATG] variants with different ribosome binding sites
       I716201 is Bca1106A.Bca1112 
I716202 is Bca1106B.Bca1112
I716203 is Bca1128A.Bca1112
I716204 is Bca1090.Bca1112
  • Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)
     I716205 is Bca1114.Bca1124 
I716206 is Bca1114.Bca1092
  • Tomorrow will make [Lox][Term][Lox] part
  • Then will put the rbsATG together with LoxTermLox

eek so many construction files and ape files to make.


to do