Samantha Liang Notebook


Revision as of 19:29, 4 June 2007 by Samanthaliang (Talk | contribs)

My Construction Files
My Sequencing Files
My Biobricks

--Samanthaliang 15:29, 4 June 2007 (EDT)

  • Oligos to make biobricks of toxins are not yet in - maybe Wednesday or Thursday?

For cassette between Cre and toxin: [rbs][ATG][Lox][Term][Lox]

  • Made [rbs][ATG] variants with different ribosome binding sites

I716201 is Bca1106A.Bca1112

       I716202 is Bca1106B.Bca1112 
I716203 is Bca1128A.Bca1112
I716204 is Bca1090.Bca1112
  • Made [Lox][Term] variants with 2 different terminators (will probably use Bca1092 version)

I716205 is Bca1114.Bca1124

      I716206 is Bca1114.Bca1092 
  • Tomorrow will make [Lox][Term][Lox] part
  • Then will put the rbsATG together with LoxTermLox

eek so many construction files and ape files to make.

to do