Samantha Liang Notebook2
From 2007.igem.org
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| + | '''For tomorrow:''' <br> | ||
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| + | ==[[User:Samanthaliang|Samanthaliang]] 15:17, 20 July 2007 (EDT)== | ||
'''Things to read about:''' <br> | '''Things to read about:''' <br> | ||
| - | *Does barnase have DNase activity? | + | *Does barnase have DNase activity? Haven't really found anything saying that it has DNase activity yet, but it is a ribonuclease that destroys all the RNA and targets the ribosome, which in turn, stops DNA synthesis such that those bacteria should no longer be able to replicate and grow. |
| - | *Is there a way to do dapi (pronounced dap-E) measurements for e.coli? dapI binds to DNA, wonder if can use a cytometer or something? Do a dapi stain? | + | *Is there a way to do dapi (pronounced dap-E) measurements for e.coli? dapI binds to DNA, wonder if can use a cytometer or something? Do a dapi stain? '''YES!''' Can do a dapi stain in e.coli and then do measurements with a flow cytometer. |
*Make sure that we're not just making a toxic protein or that Cre itself is toxic or something like that. | *Make sure that we're not just making a toxic protein or that Cre itself is toxic or something like that. | ||
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| + | So I'm having a lot of trouble isolating the plasmid of 411B | ||
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*Miniprep 408C, 408E, and 411B | *Miniprep 408C, 408E, and 411B | ||
*Sequence 408C, 408E, and 411B | *Sequence 408C, 408E, and 411B | ||
Revision as of 19:17, 20 July 2007
My Construction Files
My Sequencing Files
My Biobricks
My -80 Stocks
For tomorrow:
Samanthaliang 15:17, 20 July 2007 (EDT)
Things to read about:
- Does barnase have DNase activity? Haven't really found anything saying that it has DNase activity yet, but it is a ribonuclease that destroys all the RNA and targets the ribosome, which in turn, stops DNA synthesis such that those bacteria should no longer be able to replicate and grow.
- Is there a way to do dapi (pronounced dap-E) measurements for e.coli? dapI binds to DNA, wonder if can use a cytometer or something? Do a dapi stain? YES! Can do a dapi stain in e.coli and then do measurements with a flow cytometer.
- Make sure that we're not just making a toxic protein or that Cre itself is toxic or something like that.
So I'm having a lot of trouble isolating the plasmid of 411B
- Miniprep 408C, 408E, and 411B
- Sequence 408C, 408E, and 411B
- Make -80s of 408C, 408E, and 411B
- Pool I716430 through I716435
- Look at spotted plates
