From 2007.igem.org

My Construction Files
My Sequencing Files
My Biobricks
My -80 Stocks

---

For tomorrow:

Samanthaliang 15:17, 20 July 2007 (EDT)

Things to read about:

• Does barnase have DNase activity? Haven't really found anything saying that it has DNase activity yet, but it is a ribonuclease that destroys all the RNA and targets the ribosome, which in turn, stops DNA synthesis such that those bacteria should no longer be able to replicate and grow.
• Is there a way to do dapi (pronounced dap-E) measurements for e.coli? dapI binds to DNA, wonder if can use a cytometer or something? Do a dapi stain? YES! Can do a dapi stain in e.coli and then do measurements with a flow cytometer.
• Make sure that we're not just making a toxic protein or that Cre itself is toxic or something like that.

So I'm having a lot of trouble isolating the plasmid of 411B

• Miniprep 408C, 408E, and 411B
• Sequence 408C, 408E, and 411B
• Make -80s of 408C, 408E, and 411B
• Pool I716430 through I716435
• Look at spotted plates