http://2007.igem.org/wiki/index.php?title=Special:Contributions/Nkumar&feed=atom&limit=50&target=Nkumar&year=&month=2007.igem.org - User contributions [en]2024-03-28T11:54:03ZFrom 2007.igem.orgMediaWiki 1.16.5http://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-23T16:05:59Z<p>Nkumar: /* October 22 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br><br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br><br />
Ran gel of PCR products<br><br />
No growth on the buddy in Boo transformation<br><br />
Religations of buddy into boo but couldnt find Buddy therefore religations halted<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
ML<br><br />
Religated buddy and Boo<br><br />
Note: when free of tasks work on poster, or presentation or wiki or call justin or erin<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
JG, MC<br><br />
Retransform Buddy and Boo34<br><br />
Transform tholase<br><br><br />
<br />
JP<br><br />
Restriction of thiolase with ECO/PST<br><br />
Ran gel<br><br />
Transform rom Bud "2" oct 12<br><br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
Re-digestions for re-ligations of boo34 and buddy<br><br />
Digest boo34 s/p<br><br />
Ran gel<br><br />
Extract tholase from oct 14 into tube labelled "THiolase band EX"<br><br />
Updated wiki<br><br><br />
<br />
To do:<br><br />
Extract gel<br><br />
Ligate with buddy<br><br />
Run ligation on gel<br><br />
Extract<br><br />
Transform<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
I'll be there at 3-NK<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:27:27Z<p>Nkumar: /* October 15 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br><br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br><br />
Ran gel of PCR products<br><br />
No growth on the buddy in Boo transformation<br><br />
Religations of buddy into boo but couldnt find Buddy therefore religations halted<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
ML<br><br />
Religated buddy and Boo<br><br />
Note: when free of tasks work on poster, or presentation or wiki or call justin or erin<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
JG, MC<br><br />
Retransform Buddy and Boo34<br><br />
Transform tholase<br><br><br />
<br />
JP<br><br />
Restriction of thiolase with ECO/PST<br><br />
Ran gel<br><br />
Transform rom Bud "2" oct 12<br><br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
Re-digestions for re-ligations of boo34 and buddy<br><br />
Digest boo34 s/p<br><br />
Ran gel<br><br />
Extract tholase from oct 14 into tube labelled "THiolase band EX"<br><br />
Updated wiki<br><br><br />
<br />
To do:<br><br />
Extract gel<br><br />
Ligate with buddy<br><br />
Run ligation on gel<br><br />
Extract<br><br />
Transform<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:25:17Z<p>Nkumar: /* October 14 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br><br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br><br />
Ran gel of PCR products<br><br />
No growth on the buddy in Boo transformation<br><br />
Religations of buddy into boo but couldnt find Buddy therefore religations halted<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
ML<br><br />
Religated buddy and Boo<br><br />
Note: when free of tasks work on poster, or presentation or wiki or call justin or erin<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
JG, MC<br><br />
Retransform Buddy and Boo34<br><br />
Transform tholase<br><br><br />
<br />
JP<br><br />
Restriction of thiolase with ECO/PST<br><br />
Ran gel<br><br />
Transform rom Bud "2" oct 12<br><br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:23:44Z<p>Nkumar: /* October 13 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br><br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br><br />
Ran gel of PCR products<br><br />
No growth on the buddy in Boo transformation<br><br />
Religations of buddy into boo but couldnt find Buddy therefore religations halted<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
ML<br><br />
Religated buddy and Boo<br><br />
Note: when free of tasks work on poster, or presentation or wiki or call justin or erin<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:22:23Z<p>Nkumar: /* October 12 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br><br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br><br />
Ran gel of PCR products<br><br />
No growth on the buddy in Boo transformation<br><br />
Religations of buddy into boo but couldnt find Buddy therefore religations halted<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:21:54Z<p>Nkumar: /* October 11 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br><br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br />
Ran gel of PCR products<br />
No growth on the buddy in Boo transformation<br />
Religations<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:21:43Z<p>Nkumar: /* October 11 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br />
Transformed Buddy in Boo 1+2 into competentent cells, plated on AMP+ plates<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br />
Ran gel of PCR products<br />
No growth on the buddy in Boo transformation<br />
Religations<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:21:15Z<p>Nkumar: /* October 12 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC, JG @ 800hrs<br />
Ran gel of PCR products<br />
No growth on the buddy in Boo transformation<br />
Religations<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:19:59Z<p>Nkumar: /* October 11 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
JP?<br><br />
PCR thiolase<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC @ 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:18:36Z<p>Nkumar: /* October 10 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
JP?<br><br />
Tholase PCR<br><br><br />
MC<br><br />
Ligated Buddy oct 5 into Boo34 digest oct 6<br><br />
Left on bench<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC @ 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:17:21Z<p>Nkumar: /* October 9 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
NK<br><br />
Loaded gel with digets from october 6, boo34 S,P<br><br />
Put the ligations from oct6 in the freezer with the tape in tray #3<br><br />
<br />
VH<br><br />
Gel extrations B0034 sp1 and boo34 sp2<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC @ 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:15:33Z<p>Nkumar: /* October 6 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
Made a to do list for the day<br><br />
<br />
NK 2pm<br><br />
Ligations of I0500 purify oct 5 and J61003<br><br />
Left on bench with green tape labelled "ligations oct6"<br><br />
Digest Boo34 with s,p<br><br />
Digests in freezer labelled "digestions B0034 S,P"<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC @ 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:13:03Z<p>Nkumar: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
JG/MC - 800hrs <br><br />
Ran gel of i0500 and buddy<br><br />
Digest #4 J61003/I0500 Digest Eco/xba with Pst to drop out of GFP<br><br />
Ran gel of I0500J61003 eco/xba<br><br><br />
ML<br><br />
Gel completd and took photos<br><br />
Buddy and I0500 digested<br><br />
Extracted gel<br><br />
Labelled I0500 purify Oct5 and Buddy Purify oct 5<br><br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC @ 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-16T17:10:30Z<p>Nkumar: /* October 4 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br><br />
Cleaned up 37 degree<br><br />
Moved 30th plates of I0500+JG and Buddy+j6 to 4 degree fridge<br><br />
Disposed of really disgusting plates<br><br />
Got ride of I0500 ovrnights that hve been on shaker for a wek now<br><br />
CHecke thoolase kan plates<br><br />
Showing blue dots so far and af we white doets<br><br />
Left plates in 37 celsious room<br><br><br />
JP<br><br />
Sequencing of 3 primer of DBS<br><br />
Ligate I0500 eco/spe into J61003 (eco/xba)<br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
<br />
<br />
CZ - Sorry I can't make it for personal reasons.<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
NG 12:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
MC @ 800hrs<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-04T19:43:25Z<p>Nkumar: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
<br />
<br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-04T19:43:14Z<p>Nkumar: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
NK - 9-11AM<br><br />
<br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-04T17:22:10Z<p>Nkumar: /* October 5 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
NK - 9-11AM<br><br />
I just wanted to drop by and do the houskeeping duties with you because im not sure what to do. Is that ok? -NK<br><br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-04T17:20:35Z<p>Nkumar: /* October 3 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
Autoclaved 2 bottosl of Ependorf tupes- to be picked up from G308<br><br />
Transform THolase into XL10 gold plates<br><br />
Miniprep of 10500+J61003 O/N<br><br />
<br />
ML<br><br />
Digest of 10500+J61003 with ECORI and XBA<br><br />
Housekeeping complete<br><br />
Note to Justin: Samples to sequence are in -20 labelled "Justin! Sequence me"<br><br />
CZ - 7:00pm<br><br />
Ran gel of I0500/J61003<br><br />
It looks like I05oo is in J61003 but have to confirm with Justin or Michelle or Erin. <br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-04T17:15:53Z<p>Nkumar: /* October 2 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br><br />
No Kan plates therefore made kan plates<br><br />
On COuntertop<br><br />
Miniprepped ML's overnights from OCt 1<br><br />
Lysis solution is a no go<br><br />
Started new O/N of previows overnights for tomorrow<br><br />
No more LB<br><br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-04T17:13:19Z<p>Nkumar: /* October 1 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
JG<br><br />
Miniprep J61003+Enny and Benny+J61003<br><br />
Minis are in -20<br><br><br />
ML<br><br />
Brought the tubes labelled "sequencing rxns" up to MBSU<br><br />
Also brought some XBA 1 from fermentas freezer since we ran out<br><br />
Digests JG's miniprep with Xbal and PST. Ran out of out of XBA during digests, which meant that EJ 4,5,6only digested with XBA for 35 min<br><br />
Colony O/N of I0500/ J61003 and Buddy/J61003<br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
VH-1PM<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - night (to be confirmed)<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-10-04T17:06:38Z<p>Nkumar: /* September 30 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br><br />
Redid miniprep of I0500 induced by IPTG(1C,1D,2C)and elute in 40 microlitre<br><br />
Ran 3microliter ona gel but nothing appeared<br><br />
Miniprep discarded because of lack of DNA<br><br />
James took 1D culture ands ub culture and perhaps induce with IPTH again.<br><br />
Restarted 1C and 2C (5ml LB, 5microlitre K, 100microlitre culture)<br><br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
Made of gel of James's Miniprep digests<br><br />
Digest I0500 with ECORI and SPE<br><br />
Transformations of Ligations Enny + J61003 and Betty + J61003<br><br />
<br />
NK 1130-230<br><br />
Loaded gel but samples were incorrectly loaded<br><br><br />
CZ 2:00 pm<br><br />
Loaded gel of Erin's digests<br><br />
Gel extraction<br><br />
Ligation with J61003 E, X<br><br />
<br />
Note:The new TAE is taking much longer to solidify when 1%. <br><br />
<br />
JP evening<br><br />
Tholase PCR<br><br />
Sequencing reactions of DB in Boo and Buddy in Boo<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br><br />
Transformed Buddy+J61003, I0500+J61003 #1 and #2<br><br />
Plated all cells on AMP<br><br />
O/Ns of Enny+J61003 and Benny+J61003<br><br />
NG 12:00 am<br><br />
<br />
JP<br><br />
Tholase blunt end topo reaction<br><br />
Completed reaction and tholase mix is in orange PCR tube rack at -20<br><br />
<br />
Note:<br><br />
1) We need XGAL!!<br><br />
2) Colonies that are blue do not have thiolase in them, colonies that are wight do have thiolase in them.<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-10-04T17:06:26Z<p>Nkumar: /* September 30 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
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border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br><br />
Redid miniprep of I0500 induced by IPTG(1C,1D,2C)and elute in 40 microlitre<br><br />
Ran 3microliter ona gel but nothing appeared<br><br />
Miniprep discarded because of lack of DNA<br><br />
James took 1D culture ands ub culture and perhaps induce with IPTH again.<br><br />
Restarted 1C and 2C (5ml LB, 5microlitre K, 100microlitre culture)<br><br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
Made of gel of James's Miniprep digests<br><br />
Digest I0500 with ECORI and SPE<br><br />
Transformations of Ligations Enny + J61003 and Betty + J61003<br><br />
<br />
NK 1130-230<br><br />
Loaded gel but samples were incorrectly loaded<br><br><br />
CZ 2:00 pm<br><br />
Loaded gel of Erin's digests<br><br />
Gel extraction<br><br />
Ligation with J61003 E, X<br><br />
<br />
Note:The new TAE is taking much longer to solidify when 1%. <br><br />
<br />
JP evening<br><br />
Tholase PCR<br><br />
Sequencing reactions of DB in Boo and Buddy in Boo<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br><br />
Transformed Buddy+J61003, I0500+J61003 #1 and #2<br><br />
Plated all cells on AMP<br><br />
O/Ns of Enny+J61003 and Benny+J61003<br><br />
NG 12:00 am<br><br />
<br />
JP<br><br />
Tholase blunt end topo reaction<br><br />
Completed reaction and tholase mix is in orange PCR tube rack at -20<br><br />
<br />
Note:<br />
1) We need XGAL!!<br><br />
2) Colonies that are blue do not have thiolase in them, colonies that are wight do have thiolase in them.<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-10-04T17:05:56Z<p>Nkumar: /* September 30 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
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border-width:1px;<br />
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font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
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<td></td><br />
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<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br><br />
Redid miniprep of I0500 induced by IPTG(1C,1D,2C)and elute in 40 microlitre<br><br />
Ran 3microliter ona gel but nothing appeared<br><br />
Miniprep discarded because of lack of DNA<br><br />
James took 1D culture ands ub culture and perhaps induce with IPTH again.<br><br />
Restarted 1C and 2C (5ml LB, 5microlitre K, 100microlitre culture)<br><br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
Made of gel of James's Miniprep digests<br><br />
Digest I0500 with ECORI and SPE<br><br />
Transformations of Ligations Enny + J61003 and Betty + J61003<br><br />
<br />
NK 1130-230<br><br />
Loaded gel but samples were incorrectly loaded<br><br><br />
CZ 2:00 pm<br><br />
Loaded gel of Erin's digests<br><br />
Gel extraction<br><br />
Ligation with J61003 E, X<br><br />
<br />
Note:The new TAE is taking much longer to solidify when 1%. <br><br />
<br />
JP evening<br><br />
Tholase PCR<br><br />
Sequencing reactions of DB in Boo and Buddy in Boo<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br><br />
Transformed Buddy+J61003, I0500+J61003 #1 and #2<br><br />
Plated all cells on AMP<br><br />
O/Ns of Enny+J61003 and Benny+J61003<br><br />
NG 12:00 am<br><br />
<br />
JP<br><br />
Tholase blunt end topo reaction<br><br />
Completed reaction and tholase mix is in orange PCR tube rack at -20<br><br />
<br />
Note 1) We need XGAL!!<br><br />
2) Colonies that are blue do not have thiolase in them, colonies that are wight do have thiolase in them.<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-10-04T17:05:15Z<p>Nkumar: /* September 30 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
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<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br><br />
Redid miniprep of I0500 induced by IPTG(1C,1D,2C)and elute in 40 microlitre<br><br />
Ran 3microliter ona gel but nothing appeared<br><br />
Miniprep discarded because of lack of DNA<br><br />
James took 1D culture ands ub culture and perhaps induce with IPTH again.<br><br />
Restarted 1C and 2C (5ml LB, 5microlitre K, 100microlitre culture)<br><br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
Made of gel of James's Miniprep digests<br><br />
Digest I0500 with ECORI and SPE<br><br />
Transformations of Ligations Enny + J61003 and Betty + J61003<br><br />
<br />
NK 1130-230<br><br />
Loaded gel but samples were incorrectly loaded<br><br><br />
CZ 2:00 pm<br><br />
Loaded gel of Erin's digests<br><br />
Gel extraction<br><br />
Ligation with J61003 E, X<br><br />
<br />
Note:The new TAE is taking much longer to solidify when 1%. <br><br />
<br />
JP evening<br><br />
Tholase PCR<br><br />
Sequencing reactions of DB in Boo and Buddy in Boo<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br><br />
Transformed Buddy+J61003, I0500+J61003 #1 and #2<br><br />
Plated all cells on AMP<br><br />
O/Ns of Enny+J61003 and Benny+J61003<br><br />
NG 12:00 am<br><br />
<br />
JP<br><br />
Tholase blunt end topo reaction<br><br />
Completed reaction and tholase mix is in orange PCR tube rack at -20<br><br />
<br />
Note: We need XGAL!!<br><br />
: Colonies that are blue do not have thiolase in them, colonies that are wight do have thiolase in them.<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-10-04T16:59:53Z<p>Nkumar: /* September 29 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
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border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
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<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br><br />
Redid miniprep of I0500 induced by IPTG(1C,1D,2C)and elute in 40 microlitre<br><br />
Ran 3microliter ona gel but nothing appeared<br><br />
Miniprep discarded because of lack of DNA<br><br />
James took 1D culture ands ub culture and perhaps induce with IPTH again.<br><br />
Restarted 1C and 2C (5ml LB, 5microlitre K, 100microlitre culture)<br><br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
Made of gel of James's Miniprep digests<br><br />
Digest I0500 with ECORI and SPE<br><br />
Transformations of Ligations Enny + J61003 and Betty + J61003<br><br />
<br />
NK 1130-230<br><br />
Loaded gel but samples were incorrectly loaded<br><br><br />
CZ 2:00 pm<br><br />
Loaded gel of Erin's digests<br><br />
Gel extraction<br><br />
Ligation with J61003 E, X<br><br />
<br />
Note:The new TAE is taking much longer to solidify when 1%. <br><br />
<br />
JP evening<br><br />
Tholase PCR<br><br />
Sequencing reactions of DB in Boo and Buddy in Boo<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br />
<br />
NG 12:00 am<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-10-04T16:53:06Z<p>Nkumar: /* September 28 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br><br />
Redid miniprep of I0500 induced by IPTG(1C,1D,2C)and elute in 40 microlitre<br><br />
Ran 3microliter ona gel but nothing appeared<br><br />
Miniprep discarded because of lack of DNA<br><br />
James took 1D culture ands ub culture and perhaps induce with IPTH again.<br><br />
Restarted 1C and 2C (5ml LB, 5microlitre K, 100microlitre culture)<br><br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
NK 1130-230<br><br />
CZ 2:00 pm<br />
<br />
JP evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br />
<br />
NG 12:00 am<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-02T16:09:18Z<p>Nkumar: /* October 6 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - 2:30pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br><br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
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<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-02T16:09:07Z<p>Nkumar: /* October 6 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - 2:30pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br />
NK 2pm<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-02T16:08:30Z<p>Nkumar: /* October 4 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br><br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - 2:30pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/OctoberAlberta/Calender/October2007-10-02T16:08:19Z<p>Nkumar: /* October 4 */</p>
<hr />
<div><div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/October|October 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<br />
<tr><br />
<td></td><br />
<td>[[Alberta/Calender/October#October_1|1]]</td><br />
<td>[[Alberta/Calender/October#October_2|2]]</td><br />
<td>[[Alberta/Calender/October#October_3|3]]</td><br />
<td>[[Alberta/Calender/October#October_4|4]]</td><br />
<td>[[Alberta/Calender/October#October_5|5]]</td><br />
<td>[[Alberta/Calender/October#October_6|6]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_7|7]]</td><br />
<td>[[Alberta/Calender/October#October_8|8]]</td><br />
<td>[[Alberta/Calender/October#October_9|9]]</td><br />
<td>[[Alberta/Calender/October#October_10|10]]</td><br />
<td>[[Alberta/Calender/October#October_11|11]]</td><br />
<td>[[Alberta/Calender/October#October_12|12]]</td><br />
<td>[[Alberta/Calender/October#October_13|13]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_14|14]]</td><br />
<td>[[Alberta/Calender/October#October_15|15]]</td><br />
<td>[[Alberta/Calender/October#October_16|16]]</td><br />
<td>[[Alberta/Calender/October#October_17|17]]</td><br />
<td>[[Alberta/Calender/October#October_18|18]]</td><br />
<td>[[Alberta/Calender/October#October_19|19]]</td><br />
<td>[[Alberta/Calender/October#October_20|20]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_21|21]]</td><br />
<td>[[Alberta/Calender/October#October_22|22]]</td><br />
<td>[[Alberta/Calender/October#October_23|23]]</td><br />
<td>[[Alberta/Calender/October#October_24|24]]</td><br />
<td>[[Alberta/Calender/October#October_25|25]]</td><br />
<td>[[Alberta/Calender/October#October_26|26]]</td><br />
<td>[[Alberta/Calender/October#October_27|27]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/October#October_28|28]]</td><br />
<td>[[Alberta/Calender/October#October_29|29]]</td><br />
<td>[[Alberta/Calender/October#October_30|30]]</td><br />
<td>[[Alberta/Calender/October#October_31|31]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/September|September 2007]]<br><br />
To [[Alberta/Calender/November|November 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== October 1 == <br><br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 3 == <br><br />
<br />
MC - 800hrs <br><br />
CZ - 7:00pm<br><br />
<br />
<b>NB: please note the lab is unavailable EVERY wednesday from 1400-1700hrs</b><br><br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 4 ==<br />
NK - 930<br />
VH - 2pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 5 ==<br />
<br />
MC - 800hrs <br><br />
CZ - 2:30pm<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 6 ==<br />
<br />
ED 9:00<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 7 ==<br />
<br />
ED 9:00<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 8 ==<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 9 ==<br />
<br />
<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 10 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 11 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 12 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 13 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 14 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 15 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 16 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 17 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 18 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 19 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 20 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 21 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 22 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 23 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 24 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
== October 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/October#October|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/September|To September 2007]]<br><br />
[[Alberta/Calender/Novemeber|To November 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-25T19:39:37Z<p>Nkumar: /* September 29 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
930-1230 NK<br />
<br />
1-ish - AL<br />
<br />
2pm-ish - VH<br />
<br />
TO DO LIST:<br />
<br />
1)<br />
<br />
looking through the book to last thursday at my sequencing reactions and figure out exactly what samples I used for X-2, X-3, and X-4?<br />
<br />
2)<br />
<br />
If there is not much left (less than 20 microL), can you transform into bacteria so we can complete another mini? (only 1 transformation for each)<br />
<br />
3) <br />
<br />
place each of those tubes in a rack or something and leave directions so wayne can find it.<br />
<br />
4)<br />
<br />
X-1 has two prefixes, so its garbage, but X-5 might be ok. I couldn't find a terminator or a suffix. So... Can you complete a restriction on X-5 with Eco/Spe and Eco/Pst and run on a gel to make sure it cuts properly (and therefore those restriction sites exist)<br />
<br />
5)<br />
<br />
run sequencing reaction on more X-1 and X-5's (look at the chart erin made from back in the day - its a fold out piece of paper in the master book)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
VH - 2pm-ish<br />
<br />
JP- evening<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
1-ish - AL<br />
<br />
CZ 2:30pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
ED 9:00 am<br><br />
NK 1130-230<br><br />
CZ 2:00 pm<br />
<br />
JP evening<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
ED 9:00 Am<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-24T07:45:01Z<p>Nkumar: /* September 27 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
930-1230 NK<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
NK 930-1230 <br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
CZ 2:30pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
CZ 2:00 pm<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-24T07:44:41Z<p>Nkumar: /* September 28 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
Poster Meeting @ 11:00am<br />
Meet in sub By the Subway--JG<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM -JG @ 8000hrs <br><br />
MC @ 845 - going to stop by dean of students' to pick up swag first.<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
930-1230 NK<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
JG & MC - 8:00AM <br><br />
<br />
CZ 2:30pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
CZ 2:00 pm<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-23T05:20:56Z<p>Nkumar: /* September 28 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
930-1230 NK<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
NK 930-1230<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-23T05:20:26Z<p>Nkumar: /* September 25 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
930-1230 NK<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-23T05:19:45Z<p>Nkumar: /* September 24 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
<br />
PM - NK @5 <br><br />
sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-23T05:19:27Z<p>Nkumar: /* September 24 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
<b>To do:</b><br><br />
miniprep of DBBEBB and DBBBBE & glycerol stocks<br><br />
restrict with (1)XBA/PST, (2)SPE/PST<br><br />
Ruun gel of UNCut DBBEBB and DBBBBE, Cut with XBA/PST and CUT with SPE/PST<br><br />
GEl extract XBA/PST bands<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
<br />
<b>if you're the first one in; I0500 arrived - pop by and give Mike a visit!:) </b><br />
<br />
MC - in the AM - unsure what time because has to be downtown for a class project @900hrs (hoping to get out of there within a hour) so maybe 10/1030? <br />
<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
<br />
PM - NK @5 sorry, cant make it till 630<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T18:03:38Z<p>Nkumar: /* September 24 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
To do for Sept 18<br><br />
To do: miniprep, of DBBEBB and DBBBBE><br><br />
restrict with XBA, PST><br><br />
Seperate restriction SPE PST><br><br />
Ruun gel of UNCut ligations><br><br />
Cut with XBA PST and CUT with SPE PST><br><br />
GEl extract XBA, PST><br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
<br />
PM - NK @5<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T18:01:30Z<p>Nkumar: /* September 20 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
To do for Sept 18<br><br />
To do: miniprep, of DBBEBB and DBBBBE><br><br />
restrict with XBA, PST><br><br />
Seperate restriction SPE PST><br><br />
Ruun gel of UNCut ligations><br><br />
Cut with XBA PST and CUT with SPE PST><br><br />
GEl extract XBA, PST><br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 930 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
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[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
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[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
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[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
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[[Alberta/Calender/September#September|to the top]]<br />
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== September 29 ==<br />
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[[Alberta/Calender/September#September|to the top]]<br />
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== September 30 ==<br />
<br />
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[[Alberta/Calender/September#September|to the top]]<br />
<br />
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[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T18:00:56Z<p>Nkumar: /* September 18 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
To do for Sept 18<br><br />
To do: miniprep, of DBBEBB and DBBBBE><br><br />
restrict with XBA, PST><br><br />
Seperate restriction SPE PST><br><br />
Ruun gel of UNCut ligations><br><br />
Cut with XBA PST and CUT with SPE PST><br><br />
GEl extract XBA, PST><br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br><br />
Sorry guys, I havent done mini-preps before so just incase I mess up i decided not do it. Instead I made a gel for tonight and update the wiki (even the missing parts from before).<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
NK-6PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T17:59:06Z<p>Nkumar: /* September 17 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br><br />
<br />
To do for Sept 18<br><br />
To do: miniprep, of DBBEBB and DBBBBE><br><br />
restrict with XBA, PST><br><br />
Seperate restriction SPE PST><br><br />
Ruun gel of UNCut ligations><br><br />
Cut with XBA PST and CUT with SPE PST><br><br />
GEl extract XBA, PST><br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T17:58:28Z<p>Nkumar: /* September 17 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)><br><br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T17:57:48Z<p>Nkumar: /* September 17 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
To do: miniprep, of DBBEBB and DBBBBE><br><br />
restrict with XBA, PST><br><br />
Seperate restriction SPE PST><br><br />
Ruun gel of UNCut ligations><br><br />
Cut with XBA PST and CUT with SPE PST><br><br />
GEl extract XBA, PST><br><br />
<br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)><br><br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T17:05:30Z<p>Nkumar: /* September 17 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)><br><br />
To do: miniprep, of DBBEBB and DBBBBE><br><br />
restrict with XBA, PST><br><br />
Seperate restriction SPE PST><br><br />
Ruun gel of UNCut ligations><br><br />
Cut with XBA PST and CUT with SPE PST><br><br />
GEl extract XBA, PST><br><br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T17:03:04Z<p>Nkumar: /* September 16 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br><br />
Transform ligations of DBBEBB and DBBBBE into competent cells. <br><br />
Plated on AMP with whole ligations<br><br />
General Info: When DBBEBB and DBBBBE are complete run sequence reaction<br><br />
Follow "flouresence sequence reaction" protocol<br><br />
<br />
11-2 VH, CZ<br><br />
<br />
2-5 MC, JP<br><br />
<br />
5-8 - ML, NG<br><br />
No growth on DBBBBE or DBBBBE at 1700<br><br />
Retransformed into competent cells DBBEBB and DBBBBE<br><br />
Plated 2 of each and lover overnight at 37 degrees<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T16:58:56Z<p>Nkumar: /* September 15 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br><br />
General Note:We have cloned all 5 genes in series, in to differnt orders. Waiting for I0500 which couls be completed on monday. To make sure we have cloned correctly will also need to wait for the squencing results. This weekdn we will clone the 5 gense in 2 differnt orders. The digest have already been done<br><br />
Gel extraction of gel bands of XBA,PST of BBDBBE and BEDBBB. THese can be used to cone into I0500 J61003<br><br />
Ligations of DBBB into BE and DBBE into DBBEBB<br><br />
Leave at 20 degrees celsius for 10 hours<br><br />
5-8 - NK<br />
Cant find the competent cells. <br><br />
Competent cells are in -80 freezer 2nd shelf from the bottom-ML, NG<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T16:52:17Z<p>Nkumar: /* September 14 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
To do: Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br />
<br />
11-2 - JG, ML<br />
<br />
2-5 - NG, CZ<br />
<br />
5-8 - NK, I have it covered.<br />
<br />
Could not do transformation ligations because could not find competent cells. The ligations are in the -20 freezer with the green tape saying "ligations, sept 15". -NK<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T16:51:48Z<p>Nkumar: /* September 14 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL, JG<br><br />
Restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
PM - VH, CZ<br><br />
Ran gel on restrictions on BBDBE and BEDBB with xba/pst and spe/pst<br><br />
Gel extracions<br><br />
<br />
night - JP<br><br />
Sequencing reactions<br><br />
BBDBBE 6 reactions<br><br />
BEDBBB 6 reactions<br><br />
BB 3 reactions<br><br />
BE 3 reactions<br><br><br />
General note: Each primer sequence will be the end of the indicated gene to show us wether the joining/ligations worked correctly (VF is the promoter/gene/juction)<br><br />
Submit to MBSU<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br />
<br />
11-2 - JG, ML<br />
<br />
2-5 - NG, CZ<br />
<br />
5-8 - NK, I have it covered.<br />
<br />
Could not do transformation ligations because could not find competent cells. The ligations are in the -20 freezer with the green tape saying "ligations, sept 15". -NK<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T16:42:38Z<p>Nkumar: /* September 13 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started using primers 1-5 that have been diluted to 5pmol/microlitre in H20 not TE<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL<br />
<br />
PM - VH, CZ<br />
<br />
night - JP<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br />
<br />
11-2 - JG, ML<br />
<br />
2-5 - NG, CZ<br />
<br />
5-8 - NK, I have it covered.<br />
<br />
Could not do transformation ligations because could not find competent cells. The ligations are in the -20 freezer with the green tape saying "ligations, sept 15". -NK<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T16:41:49Z<p>Nkumar: /* September 13 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Digested BEBOO and BBBOO with PST and XBA<br />
<br />
PM - VH, JG<br><br />
Ran gel of restrictions made in the previous shift.<br><br />
<br />
Meeting 7:00<br />
<br />
After lab shift, JG, JP<br><br />
Overnights with AMP of BBDB + BEDB<br><br />
Gel extractions from gel made in the prevous shift<br><br />
bands 1,2 (BE) correct while band 3 is too large to be BB<br><br />
Sequence reactions can now be started<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL<br />
<br />
PM - VH, CZ<br />
<br />
night - JP<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br />
<br />
11-2 - JG, ML<br />
<br />
2-5 - NG, CZ<br />
<br />
5-8 - NK, I have it covered.<br />
<br />
Could not do transformation ligations because could not find competent cells. The ligations are in the -20 freezer with the green tape saying "ligations, sept 15". -NK<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumarhttp://2007.igem.org/wiki/index.php/Alberta/Calender/SeptemberAlberta/Calender/September2007-09-18T16:38:00Z<p>Nkumar: /* September 12 */</p>
<hr />
<div>=='''September'''==<br />
<br />
<div valign="center"> <br />
<table><tr><td><table style="<br />
font-family: Verdana, Arial, Helvetica, sans-serif;<br />
vertical-align:middle;<br />
text-align:center;<br />
background-color:white;<br />
border-color:#FFFFFF;<br />
border-width:1px;<br />
color:black;<br />
font-size: 12px;"><br />
<tr><br />
<td colspan="7" style="font-weight: bold;<br />
width:170px;<br />
height:24px;<br />
color: black;">[[Alberta/Calender/September|September 2007]]</td><br />
</tr><br />
<tr><br />
<td>Su</td><br />
<td>M</td><br />
<td>Tu</td><br />
<td>W</td><br />
<td>Th</td><br />
<td>F</td><br />
<td>Sa</td><br />
</tr><br />
<tr><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td>[[Alberta/Calender/September#September_1|1]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_2|2]]</td><br />
<td>[[Alberta/Calender/September#September_3|3]]</td><br />
<td>[[Alberta/Calender/September#September_4|4]]</td><br />
<td>[[Alberta/Calender/September#September_5|5]]</td><br />
<td>[[Alberta/Calender/September#September_6|6]]</td><br />
<td>[[Alberta/Calender/September#September_7|7]]</td><br />
<td>[[Alberta/Calender/September#September_8|8]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_9|9]]</td><br />
<td>[[Alberta/Calender/September#September_10|10]]</td><br />
<td>[[Alberta/Calender/September#September_11|11]]</td><br />
<td>[[Alberta/Calender/September#September_12|12]]</td><br />
<td>[[Alberta/Calender/September#September_13|13]]</td><br />
<td>[[Alberta/Calender/September#September_14|14]]</td><br />
<td>[[Alberta/Calender/September#September_15|15]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_16|16]]</td><br />
<td>[[Alberta/Calender/September#September_17|17]]</td><br />
<td>[[Alberta/Calender/September#September_18|18]]</td><br />
<td>[[Alberta/Calender/September#September_19|19]]</td><br />
<td>[[Alberta/Calender/September#September_20|20]]</td><br />
<td>[[Alberta/Calender/September#September_21|21]]</td><br />
<td>[[Alberta/Calender/September#September_22|22]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_23|23]]</td><br />
<td>[[Alberta/Calender/September#September_24|24]]</td><br />
<td>[[Alberta/Calender/September#September_25|25]]</td><br />
<td>[[Alberta/Calender/September#September_26|26]]</td><br />
<td>[[Alberta/Calender/September#September_27|27]]</td><br />
<td>[[Alberta/Calender/September#September_28|28]]</td><br />
<td>[[Alberta/Calender/September#September_29|29]]</td><br />
</tr><br />
<tr><br />
<td>[[Alberta/Calender/September#September_30|30]]</td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
<td></td><br />
</tr><br />
<br />
<br />
</table></td><td><br />
</td></tr></table></div><br />
<br />
<br />
To [[Alberta/Calender/August|August 2007]]<br><br />
To [[Alberta/Calender/October|October 2007]]<br><br />
Back to [[Alberta|UofA iGEM Home]]<br><br />
<br />
== September 1 ==<br />
<br><br />
<br />
<b> Lab Notes </b><br><br />
1. 24 Minipreps for Diezel Blaze in B0034 using Fermentas Kit; stored in -20 freezer<br><br />
2. Lots of colonies from the transformation last night; no colonies on negation control (good) and lots of colonies on positive control (good) and no satellite colonies (also good); no colonies were picked for overnights<br><br />
3. Run the Buddy+Benny in B00 and Betty+Enny in B00 Pst/Xba gel from last night at 50V for another 30 minutes for better resolution; all the digest worked so I cut out the appropriate bands and stored the gel in -20 freezer for purification later<br><br />
<br />
-CZ<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 2 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 3 ==<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 4 ==<br />
<b>Schedule:</b><br />
<br />
JP,ML,JG 7:00pm <br><br />
<br />
<br />
<u>For the record: </u><br><br />
Buddy = BuOH DH 1235 bp <br><br />
Betty = Bb-bhBu-coa dh 923 bp <br><br />
Benny = Butyryl coa dh 1184 bp <br><br />
enny = Enoyl coa Hydratase 860 bp <br><br />
Diezel Blaze = buAld-Dh 2639 bp<br><br />
<br />
<b> Notes:</b> <br><br />
Moving was done this mid-day. <br><br />
New location <b>M349</b> <br><br />
JG has key & access <br><br />
- MC, ED, JG <br><br />
<br />
Evening crew - please do a once over of G308 to double check we have not left anything and to clean up anything extra/ things we should get out of the way. thnx - MC <br><br />
<br />
<b>Night Crew</b><br />
<br />
-JP, JG<br />
<br />
Set up new lab <br />
<br />
Things we need <br />
- Water Bath, Scale, disposable 13ml overnight tubes, Tip waste<br />
<br />
<b> lab work </b><br />
<br />
Gel purification ran on Betty,enny and boo as well as buddy and benny and is in the -20 in the new lab<br />
<br />
To Do list for tomorrow<br />
<br />
- Restriction on DB needs to be done - If we want to put it in front of BE or BB then Spe/Pst - If we want to put it behind BE or BB then cut with Xba/Pst - maybe do both (use 4microL DNA for digest)<br><br />
<br />
-complete restriction on BE and BB for SPE and PST (then we can complete ligations BE-BB and BB-BE or we can ligate D.B into BE or BB to make BE-DB or DB-BE or BB-DB or DB-BB- Then we can ligate BE-BB-DB or DB-BE-BB or BE-BB-DB or BB-DB-BE or BB-BE-DB and we are kinda finished! <br><br />
<br />
- If I0500 comes in from calgary it needs to be transformed.<br />
<br />
- Transform R0080 which is a back up promoter just in case?<br />
<br />
<br />
<br />
-Note we also have to do sequenceing on BB and BE and DB<br />
<br />
Shout out to calgary for saving our necks <br />
Shout out to the move in crew <br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 5 ==<br />
<br />
<b>Schedule:</b><br />
<br />
NG,ED,ML 7:00pm <br><br />
<br />
<b>AM Crew Notes:</b><br><br />
<br />
finished up cleaning up post-move in<br> <br />
got a 'water bath' - JG calibrated it<br><br />
prepared BB in Boo & BE in Boo for sequencing - this will be ready for pick up tomorrow<br><br />
Restricted:<br><br />
- DB in BOO with PST/XBA<br><br />
- DB in BOO with PST/SPE<br><br />
- BB in BOO with PST/SPE<br><br />
- BE in BOO with PST/SPE<br><br />
<b>NB: there is a section in the masterbook "figuring stuff out" just a few questions I have from being away so long - don't worry about it we should do a wet lab recap to bring everyone on the same page at our meeting Thrusday - MC </b> <br><br />
<3 JG & MC <br><br />
<br />
Transformed I0500 from Calgary.<br />
Transformed R0080.<br />
Ran digestions on gel.<br />
Ligated digested DB with BB and BE. (all in Boo)<br />
<br />
-ED, ML<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 6 ==<br />
<b>Schedule:</b><br />
<br />
NK ED AL 5:00pm (Unless your crazy enough to show up at 5AM)<br><br />
<br />
<b>To Do:</b><br><br />
Ligations<br><br />
<br />
<B> Goodies will be served during the meeting (7:00pm @ CAB 373), so be sure to show up! </b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 7 ==<br />
<b>Schedule:</b><br />
<br />
AM Crew: MC, JG <b>meeting at 800hrs</b><br><br />
<br />
<b>Lab Notes:</b><br><br />
No growth observed for R0080 overnights; just scrapping it because we have I0500<br><br />
No growth on transformations of BB+DB and BE+DB ligations therefore . . . religated - see masterbook for more details<br><br />
O/N with AMP for I0500<br><br />
Transform religations in to XL10 Gold<br><br />
-MC, JG, ML<br />
<br />
<b>NB: JG has key access</b><br><br />
<br />
<b>To Do:</b><br><br />
Mini preps on I0500 overnights<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 8 ==<br />
<b>Schedule:</b><br />
<br />
Super Awesome Marathon of Productiveness<br />
<br />
8-11:<br />
JG,NG<br><br />
Miniprep I0500<br><br />
DB BE and DB-BB transformations did not work.<br><br />
Re-ligated DB into BB boo and DB into BE boo<br><br />
<br />
<br />
11-2:<br />
AL,ML<br><br />
Re-transform BE+DB and BB+DB<br><br />
<br />
2-5:<br />
VH<br><br />
Gel extraction of I0500, but we missed the step "restricting with Ecori+speI" <br><br />
<br />
5-8:<br />
JP,NK<br><br />
Restrction on I0500 mini's with Ecori and SPeI<br><br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 9 ==<br />
<b>Schedule:</b><br />
<br />
And Yet another super productive Sunday<br />
<br />
8-11: NG and CZ<br />
<br />
NG - Nobody Called me about the key last night (and I completely forgot to swing by: was with parents). So I will wait by the lab either , until the next group shows up or someone with a key. Also bioSci is locked but there is an open door by the physcology wing. I have my cell on 902-8032.<br />
<br />
NG - Update: 9:30am 'broke' into lab :D. Then found key...<br />
<br />
11-2: VH, CZ<br><br />
Nick ran the gel of I0500 restricited by Eco/Spe, but nothing showed up as expected (1.5kb). <br><br />
Digested I0500 and J61003 with Eco/Pst as instructed.<br><br />
<br />
2-5: MC, JG<br><br />
ran gel of restricted I0500 & J61003; unfortunately there is no DNA in the I0500 sample either<br><br />
gel extracted J61003<br><br />
Made LB - to be divided into bottles & autoclaved in G308<br><br />
O/N of DB in BB & DB in BE samples left in 37degree room<br><br />
<br />
5-8: ML, ED<br />
autoclaved little bottles of LB. NOTE: does not need to be autoclaved before you pour it into little bottles and then re-autoclave. Just pour into bottles and autoclave once<br />
digested all in Boos except for DB with Pst/xba<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 10 ==<br />
<b>Schedule:</b><br />
And Scheduling just got hard core<br />
<br />
I have sheduled what we have taken down in the meeting some people appear many times on this schedule working alone just because of the time they were availible. If you are comfortable working alone and taking on lots of shifts im sure the team would be greatful but I'm also sure we would also understand if you didn't want to work alone in the lab. I have only scheduled this way because we need man hours so i tried to fill up all the spots as best i could.<br />
-JG<br />
<br />
MC,JG for the AM<br><br />
put away bottled LB<br><br />
Ran gel of last night's restricted samples "in boo"; did not really turn out<br><br />
ran gel of uncut & single restricted I0500; uncut I0500 showed 2 bands and restricted showed nothing<br><br />
Mini prepped Ligations of DB in BB and DB in BE<br><br />
<br />
to do:<br />
- run gel of double digested I0500<br><br />
- do single & double restriction on Mini prep of Ligations<br><br />
- run gel of uncut, single & double restrictions of mini prepped ligations<br><br />
<br />
ML,AL for the MID <b>(AM crew: What time will you guys be at the lab till? I won't be there until 12:30. Might need to arrange something in order to pick up keys. - AL)</b><br><br />
Double digest of BB-DB/BE-DB XBA/PST<br><br />
<br />
NG (2:30-4) for the PM (celine is in calgary if you dont feel comfortable in the lab by yourself I can show up - JG)<br />
Jason if you have time to show up that would be sweet, but if you don't I understand. -NG<br><br />
Ran gel of the restrictions made in MID, Sept 10 by ML and AL.<br />
<br />
<br />
<br />
JP,ED for the Night<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 11 ==<br />
<br />
<b>Schedule:</b><br />
No one else really signed up for tuesdays so anyone who has time to drop in should<br />
<br />
AM- NK<br />
Restrictions of BB+DB with XBA/PST and BE+DB with PST, SPE<br />
<br />
Mid - AL (12:30 - 2 ish), NG (random)<br><br />
Ran gel on restrictions made by NK in AM of Sept 11<br />
<br />
Night- ED JP<br><br />
Gel extractions<br><br />
Ligations of DB+BB + BE and DB+BE + BB<br><br />
<b> mini relocation 1 bench back, do not use the first 3 benches of the lab or the blackboard from now on!</b><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 12 ==<br />
<b>Schedule:</b><br />
<br />
NG <b> Hi guys I have a meeting 3:30 that cannot be reseached I moved myself to come in Tuesday instead (when nobody was in).</b><br />
<br />
AM - <br />
<br />
Mid- ML, AL<br />
<br />
<b> Important: Please evacuate lab before 2:00pm as there is a lab class starting at this time till 5pm! -AL</b><br />
<br />
PM - VH <br><br />
lab class 2-5<br />
<b> VH: please make note of the above announcement regarding lab class at 2:00pm </b><br />
<br />
Night - CZ, ED<br><br />
Transformed BEDBB and BBDB into XL 10 gold<br><br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 13 ==<br />
<b>Schedule:</b><br />
<br />
AM-Nik<br />
<br />
Hey guys, i wont be able to make it till 930 AM-NK<br />
<br />
PM - VH, JG<br />
<br />
Meeting 7:00<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 14 ==<br />
<br />
<b>Schedule:</b><br />
<br />
AM - MC (around 900/930hrs)<br><br />
- Mini prep from O/N of BBDBE & BEDBB<br><br />
- Glycerol stocks of BBDBE & BEDBB, and restreaked quartered plates<br><br />
- Gel extraction of BE & BB Xba/Pst<br><br />
- made gel<br> <br />
<br />
Mid - AL<br />
<br />
PM - VH, CZ<br />
<br />
night - JP<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 15 ==<br />
<b>Schedule:</b><br />
<br />
And yet another crazy weekend...<br />
<br />
8-11 - ED,MC<br />
<br />
11-2 - JG, ML<br />
<br />
2-5 - NG, CZ<br />
<br />
5-8 - NK, I have it covered.<br />
<br />
Could not do transformation ligations because could not find competent cells. The ligations are in the -20 freezer with the green tape saying "ligations, sept 15". -NK<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 16 ==<br />
<b>Schedule:</b><br />
Continued...<br />
<br />
8-11 ED, JG<br />
<br />
11-2 VH, CZ<br />
<br />
2-5 MC, JP<br />
<br />
5-8 - ML, NG<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 17 ==<br />
<br />
AM - JG (800 hrs - 930hrs),MC (@ 800hrs)<br><br />
- delivered MSBU sequencing samples<br><br />
- O/days of Ligations from yesterday<br><br />
<br />
<b>NB: AMP plates are marked with a red streak down the SIDE of the plate. if it does not have this - then it is NOT AMP</b><br><br />
<br />
ML (~10:30-1:00)<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 18 ==<br />
NK - 8 AM<br />
<br />
VH - (@1pm)<br />
<br />
AL - 12-2pm<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 19 ==<br />
ML - ~10:30 - 1:00<br />
<br />
AL - ~1:00 - 2:00<br />
<br />
Last Wednesday there was a lab class in our lab from 2-5, is this going to be a regular occurence? -VH<br />
<br />
YES - MC <br />
<br />
VH, see notes on Sept 12. - AL<br />
<br />
ED- 6PM <br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 20 ==<br />
NK - 8 AM<br />
<br />
VH - (@1PM)<br />
<br />
JG, MC- (@5PM)<br />
<br />
JP- late<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 21 ==<br />
<br />
AM - MC, JG (@ 800hrs), <br />
<br />
ML (~10:30 - 1:00)<br><br />
<br />
AL - ~12:30 - 2:00<br />
<br />
CZ-2:15PM <br> <br />
ED - 4 PM<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 22 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
JP evening<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 23 ==<br />
<br />
ED 9-12<br />
<br />
NG 12-3<br />
<br />
NK 230-530<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 24 ==<br />
<br />
<br />
AM - MC @ 8000hrs<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 25 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 26 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 27 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 28 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 29 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
== September 30 ==<br />
<br />
<br />
<br />
[[Alberta/Calender/September#September|to the top]]<br />
<br />
<br />
<br />
[[Alberta|UofA iGEM Home]]<br><br />
[[Alberta/Calender/August|To August 2007]]<br></div>Nkumar