http://2007.igem.org/wiki/index.php?title=Special:Contributions/Yfchang&feed=atom&limit=50&target=Yfchang&year=&month=
2007.igem.org - User contributions [en]
2024-03-29T11:31:32Z
From 2007.igem.org
MediaWiki 1.16.5
http://2007.igem.org/wiki/index.php/Jamboree/Compete/Project_tracks
Jamboree/Compete/Project tracks
2007-10-26T13:48:16Z
<p>Yfchang: /* Environment */</p>
<hr />
<div>{{TOCright}}<br />
Teams are separated into one of several broad categories based on the focus of their project, called tracks, to facilitate the judging process and organization of the Jamboree. The Judging committee has formed the following tracks:<br />
<br />
# '''Foundational Research''' - basic science and engineering research<br />
# '''Information Processing''' - genetically encoded control, logic, and memory<br />
# '''Energy''' - biological fuels, feedstocks, and other energy projects<br />
# '''Environment''' - sensing or remediation of environmental state<br />
# '''Health & Medicine''' - applied projects with the goal of directly improving the human condition<br />
<br />
<br />
Each track will be assigned to one room for the presentation portion of the Jamboree. All of the teams in each particular track will give their presentations in the assigned room (rooms assignments will be determined at a later date). The Judging committee will be broken down into 3-5 judges per track and will preside over the room that their track has been assigned to. This means that you will be judged against your peers in your designated track by a small subset of the Judging committee. At the end of the posters and presentations, the judges for each track will discuss and choose a 1st, 2nd, and 3rd place winner for each of the 5 tracks. So, for example, Foundational Research will have 1st, 2nd, and 3rd place trophies, and so on with each track. Awards for each track will be presented at the beginning of the awards ceremony on Sunday, Nov 4. Then the five (5) 1st place winners will give their presentations again, this time for the whole audience to see. The judges will then reconvene to decide on the iGEM 2007 Jamboree Grand Prize winner. <br />
<br />
<br />
Each team has already been assigned to a track by the Judging committee. ''If you think that you belong in a different track'', please send an email to iGEM [AT] mit [dot] edu with the subject "Tracks" by <font color=crimson>Friday Oct 26 at 12:00pm EST</font>. See which track your team has been assigned to by looking for your team in one of the lists below. <br />
<br />
<br />
<br />
====Foundational Research====<br />
* Bangalore<br />
* Bologna<br />
* Calgary<br />
* Caltech<br />
* Cambridge<br />
* Chiba<br />
* CSHL<br />
* Duke<br />
* Freiburg<br />
* Michigan<br />
* Purdue<br />
* Valencia<br />
<br />
<br />
<br />
====Information Processing====<br />
* Bay Area RSI<br />
* Davidson/Missouri<br />
* ETHZ<br />
* Mexico<br />
* Peking<br />
* Tianjin<br />
* Tokyo<br />
* Toronto<br />
* Tsinghua<br />
* UCSF<br />
* USTC<br />
* Waterloo<br />
<br />
<br />
<br />
====Energy====<br />
* Alberta<br />
* Berkeley/LBL<br />
* Boston University<br />
* Mississippi State University<br />
* Penn State University<br />
* University of Virginia<br />
* University of Missouri- Rolla<br />
<br />
<br />
<br />
<br />
====Environment====<br />
* [[Brown]]<br />
* [[Colombia/Israel]]<br />
* [[Edinburgh]]<br />
* [[Glasgow]]<br />
* [[Lethbridge]]<br />
* [[MIT]]<br />
* [[Naples]]<br />
* [[Prairie View]]<br />
* [[Southern Utah]]<br />
* [[St. Petersburg]]<br />
* [[Turkey]]<br />
<br />
====Health & Medicine====<br />
* [[Harvard]]<br />
* [[Imperial]]<br />
* [[Ljubljana]]<br />
* [[McGill]]<br />
* [[Melbourne]]<br />
* [[Paris]]<br />
* [[Princeton]]<br />
* [[Rice]]<br />
* [[Taipei]]<br />
* [[UC Berkeley]]<br />
* [[UW Madison]]<br />
* [[Virginia Tech]]</div>
Yfchang
http://2007.igem.org/wiki/index.php/Jamboree/Compete/Project_tracks
Jamboree/Compete/Project tracks
2007-10-26T13:44:36Z
<p>Yfchang: /* Health & Medicine */</p>
<hr />
<div>{{TOCright}}<br />
Teams are separated into one of several broad categories based on the focus of their project, called tracks, to facilitate the judging process and organization of the Jamboree. The Judging committee has formed the following tracks:<br />
<br />
# '''Foundational Research''' - basic science and engineering research<br />
# '''Information Processing''' - genetically encoded control, logic, and memory<br />
# '''Energy''' - biological fuels, feedstocks, and other energy projects<br />
# '''Environment''' - sensing or remediation of environmental state<br />
# '''Health & Medicine''' - applied projects with the goal of directly improving the human condition<br />
<br />
<br />
Each track will be assigned to one room for the presentation portion of the Jamboree. All of the teams in each particular track will give their presentations in the assigned room (rooms assignments will be determined at a later date). The Judging committee will be broken down into 3-5 judges per track and will preside over the room that their track has been assigned to. This means that you will be judged against your peers in your designated track by a small subset of the Judging committee. At the end of the posters and presentations, the judges for each track will discuss and choose a 1st, 2nd, and 3rd place winner for each of the 5 tracks. So, for example, Foundational Research will have 1st, 2nd, and 3rd place trophies, and so on with each track. Awards for each track will be presented at the beginning of the awards ceremony on Sunday, Nov 4. Then the five (5) 1st place winners will give their presentations again, this time for the whole audience to see. The judges will then reconvene to decide on the iGEM 2007 Jamboree Grand Prize winner. <br />
<br />
<br />
Each team has already been assigned to a track by the Judging committee. ''If you think that you belong in a different track'', please send an email to iGEM [AT] mit [dot] edu with the subject "Tracks" by <font color=crimson>Friday Oct 26 at 12:00pm EST</font>. See which track your team has been assigned to by looking for your team in one of the lists below. <br />
<br />
<br />
<br />
====Foundational Research====<br />
* Bangalore<br />
* Bologna<br />
* Calgary<br />
* Caltech<br />
* Cambridge<br />
* Chiba<br />
* CSHL<br />
* Duke<br />
* Freiburg<br />
* Michigan<br />
* Purdue<br />
* Valencia<br />
<br />
<br />
<br />
====Information Processing====<br />
* Bay Area RSI<br />
* Davidson/Missouri<br />
* ETHZ<br />
* Mexico<br />
* Peking<br />
* Tianjin<br />
* Tokyo<br />
* Toronto<br />
* Tsinghua<br />
* UCSF<br />
* USTC<br />
* Waterloo<br />
<br />
<br />
<br />
====Energy====<br />
* Alberta<br />
* Berkeley/LBL<br />
* Boston University<br />
* Mississippi State University<br />
* Penn State University<br />
* University of Virginia<br />
* University of Missouri- Rolla<br />
<br />
<br />
<br />
<br />
====Environment====<br />
* Brown<br />
* Colombia/Israel<br />
* Edinburgh<br />
* Glasgow<br />
* Lethbridge<br />
* MIT<br />
* Naples<br />
* Prairie View<br />
* Southern Utah<br />
* St. Petersburg<br />
* Turkey<br />
<br />
<br />
<br />
====Health & Medicine====<br />
* [[Harvard]]<br />
* [[Imperial]]<br />
* [[Ljubljana]]<br />
* [[McGill]]<br />
* [[Melbourne]]<br />
* [[Paris]]<br />
* [[Princeton]]<br />
* [[Rice]]<br />
* [[Taipei]]<br />
* [[UC Berkeley]]<br />
* [[UW Madison]]<br />
* [[Virginia Tech]]</div>
Yfchang
http://2007.igem.org/wiki/index.php/Jamboree/Compete/Project_tracks
Jamboree/Compete/Project tracks
2007-10-26T13:43:37Z
<p>Yfchang: </p>
<hr />
<div>{{TOCright}}<br />
Teams are separated into one of several broad categories based on the focus of their project, called tracks, to facilitate the judging process and organization of the Jamboree. The Judging committee has formed the following tracks:<br />
<br />
# '''Foundational Research''' - basic science and engineering research<br />
# '''Information Processing''' - genetically encoded control, logic, and memory<br />
# '''Energy''' - biological fuels, feedstocks, and other energy projects<br />
# '''Environment''' - sensing or remediation of environmental state<br />
# '''Health & Medicine''' - applied projects with the goal of directly improving the human condition<br />
<br />
<br />
Each track will be assigned to one room for the presentation portion of the Jamboree. All of the teams in each particular track will give their presentations in the assigned room (rooms assignments will be determined at a later date). The Judging committee will be broken down into 3-5 judges per track and will preside over the room that their track has been assigned to. This means that you will be judged against your peers in your designated track by a small subset of the Judging committee. At the end of the posters and presentations, the judges for each track will discuss and choose a 1st, 2nd, and 3rd place winner for each of the 5 tracks. So, for example, Foundational Research will have 1st, 2nd, and 3rd place trophies, and so on with each track. Awards for each track will be presented at the beginning of the awards ceremony on Sunday, Nov 4. Then the five (5) 1st place winners will give their presentations again, this time for the whole audience to see. The judges will then reconvene to decide on the iGEM 2007 Jamboree Grand Prize winner. <br />
<br />
<br />
Each team has already been assigned to a track by the Judging committee. ''If you think that you belong in a different track'', please send an email to iGEM [AT] mit [dot] edu with the subject "Tracks" by <font color=crimson>Friday Oct 26 at 12:00pm EST</font>. See which track your team has been assigned to by looking for your team in one of the lists below. <br />
<br />
<br />
<br />
====Foundational Research====<br />
* Bangalore<br />
* Bologna<br />
* Calgary<br />
* Caltech<br />
* Cambridge<br />
* Chiba<br />
* CSHL<br />
* Duke<br />
* Freiburg<br />
* Michigan<br />
* Purdue<br />
* Valencia<br />
<br />
<br />
<br />
====Information Processing====<br />
* Bay Area RSI<br />
* Davidson/Missouri<br />
* ETHZ<br />
* Mexico<br />
* Peking<br />
* Tianjin<br />
* Tokyo<br />
* Toronto<br />
* Tsinghua<br />
* UCSF<br />
* USTC<br />
* Waterloo<br />
<br />
<br />
<br />
====Energy====<br />
* Alberta<br />
* Berkeley/LBL<br />
* Boston University<br />
* Mississippi State University<br />
* Penn State University<br />
* University of Virginia<br />
* University of Missouri- Rolla<br />
<br />
<br />
<br />
<br />
====Environment====<br />
* Brown<br />
* Colombia/Israel<br />
* Edinburgh<br />
* Glasgow<br />
* Lethbridge<br />
* MIT<br />
* Naples<br />
* Prairie View<br />
* Southern Utah<br />
* St. Petersburg<br />
* Turkey<br />
<br />
<br />
<br />
====Health & Medicine====<br />
* [[Harvard]]<br />
* Imperial<br />
* Ljubljana<br />
* McGill<br />
* Melbourne<br />
* Paris<br />
* Princeton<br />
* Rice<br />
* Taipei<br />
* UC Berkeley<br />
* UW Madison<br />
* Virginia Tech</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Team_Members
NYMU Taipei/Team Members
2007-10-26T00:34:29Z
<p>Yfchang: </p>
<hr />
<div><html><br />
<p><span class="mw-headline"><font color="#0000ff" size="5"><font color="#ff0000" size="6"><img alt="" src="/iGEM2007/FCKeditor/editor/images/smiley/msn/regular_smile.gif" />&nbsp;An open mind</font>&nbsp;is the best way to look at the world.</font></span></p><br />
<p><span class="mw-headline"><font color="#000000" size="1">- Heathrow Airport, London (2007)</font></span></p><br />
<hr /><br />
<p><img alt="" src="http://igem.ym.edu.tw/iGEM2007/images/3/35/Nymu_igem2007_0614.jpg" /></p><br />
<hr width="100%" size="2" /><br />
<p>&nbsp;</p><br />
<h2><span class="mw-headline"><font color="#ff0000" size="4">Faculty Advisers</font></span></h2><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="Image:UCY.jpg" href="http://igem.ym.edu.tw/iGEM2007/index.php/Image:Image_UCY.jpg"><img height="122" alt="Image:UCY.jpg" width="92" longdesc="/mediawiki/index.php/Image:UCY.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/b/b9/Image_UCY.jpg" /></a></td><br />
<td><a class="external text" title="http://gel.ym.edu.tw/~chc/" rel="nofollow" href="http://bmi.ym.edu.tw/wp/?page_id=29"><strong>Dr. Ueng-Cheng Yang</strong></a> (楊永正)<br />
<ul><br />
<li>Associate professor, Institute of Bioinformatics, National Yang Ming University</li><br />
<li>Director of Center for Systems and Synthetic Biology</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="Image:CHC.jpg" href="http://igem.ym.edu.tw/iGEM2007/images/3/34/Chc.jpg"><img height="122" alt="Image:CHC.jpg" width="92" longdesc="/mediawiki/index.php/Image:CHC.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/3/34/Chc.jpg" /></a></td><br />
<td><a class="external text" title="http://gel.ym.edu.tw/" rel="nofollow" href="http://gel.ym.edu.tw/members.htm"><strong>Dr. Chuan-Hsiung Chang</strong></a> (張傳雄)<br />
<ul><br />
<li>Assistant professor, Institute of Bioinformatics, National Yang Ming University</li><br />
<li>Director of Bioinformatics Program</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<h2><span class="mw-headline"><font color="#ff0000" size="4">Graduate Consultants</font></span></h2><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/index.php/Image:Image_IAN.jpg"><img height="126" alt="" width="92" longdesc="/mediawiki/index.php/Image:IAN.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/b/ba/Image_IAN.jpg" /></a></td><br />
<td><a class="external text" title="http://gel.ym.edu.tw/~ian/" rel="nofollow" href="http://gel.ym.edu.tw/%7Eian/"><strong>Mr. Yi-Feng Chang</strong></a> (張益峯)<br />
<ul><br />
<li>Graduate student, Institute of Bioinformatics, National Yang Ming University</li><br />
<li>Computer science background</li><br />
<li>Works on genome organization</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/index.php/Image:Image_PETT.jpg"><img height="112" alt="" width="92" longdesc="/mediawiki/index.php/Image:PETT.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/1/15/Image_PETT.jpg" /></a></td><br />
<td><a class="external text" title="http://gel.ym.edu.tw/~pett/" rel="nofollow" href="http://gel.ym.edu.tw/%7Epett/"><strong>Mr. Wei-Chao Liao</strong></a> (廖威超)<br />
<ul><br />
<li>Graduate student, Institute of Biotechnology in Medicine, National Yang Ming University</li><br />
<li>Life sciences background</li><br />
<li>Works on viral genomes&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/index.php/Image:Image_CHY.jpg"><img height="139" alt="" width="92" longdesc="/mediawiki/index.php/Image:CHY.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/8/85/Image_CHY.jpg" /></a></td><br />
<td><a class="external text" title="http://gel.ym.edu.tw/~blent/" rel="nofollow" href="http://gel.ym.edu.tw/%7Eblent/"><strong>Mr. Chih-Hsien Yang</strong></a> (楊志賢)<br />
<ul><br />
<li><font size="2">Graduate student, Institute of Bioinformatics, National Yang Ming University </font></li><br />
<li><font size="2">Electrical engineering background </font></li><br />
<li><font size="2">Works on genomes of photosynthetic organisms</font></li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/d/d1/YHL.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:YHL.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/d/d1/YHL.jpg" /></a></td><br />
<td><strong>Mr.&nbsp;Yi-Hsien Lin</strong>&nbsp;(林宜賢)<br />
<ul><br />
<li><font size="2">Graduate student, Institute of Traditional Medicine, National Yang Ming University </font></li><br />
<li><font size="2">Medicine&nbsp;background </font></li><br />
<li><font size="2">Works on&nbsp;radiation oncology</font></li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/d/db/HPL.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:HPL.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/d/db/HPL.jpg" /></a></td><br />
<td><strong>Mr. Hua-Peng Liaw</strong> (廖華鵬)<br />
<ul><br />
<li>Graduate student, Institute of Genome Sciences, National Yang Ming University</li><br />
<li>Life sciences background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/5/50/HYW.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:HYW.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/5/50/HYW.jpg" /></a></td><br />
<td><strong>Miss Hsin-Yen&nbsp;Wang</strong> (王心妍)<br />
<ul><br />
<li>Research assistant, Institute of Biomedical Engineering, National Yang Ming University</li><br />
<li>Life sciences background&nbsp;</li><br />
<li>MSN: <a href="mailto:aeolous_wang@hotmail.com">aeolous_wang@hotmail.com</a></li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/b/b0/THK.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:THK.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/b/b0/THK.jpg" /></a></td><br />
<td><strong>Mr. Ti-Hsuan&nbsp;Ku</strong> (顧迪璿)<br />
<ul><br />
<li>Research assistant, Institute of Bioinformatics, National Yang Ming University</li><br />
<li>Life sciences background</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p>&nbsp;<span class="mw-headline"><font color="#ff0000" size="4">Undergraduate Students</font></span></p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/e/e3/YCL.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:YCL.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/e/e3/YCL.jpg" /></a></td><br />
<td><strong>Mr. Ying-Chun&nbsp;Lo</strong> (羅穎駿)<br />
<ul><br />
<li>Senior, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine&nbsp;background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/d/d7/RHC.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:RHC.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/d/d7/RHC.jpg" /></a></td><br />
<td><strong>Miss Ruo-Hsuan&nbsp;Chen</strong> (陳若萱)<br />
<ul><br />
<li>Senior, Department of Dentistry, National Yang Ming University</li><br />
<li>Dentistry&nbsp;background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/9/9e/CKD.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:CKD.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/9/9e/CKD.jpg" /></a></td><br />
<td><strong>Miss Chien-Kuang&nbsp;Ding</strong> (丁千光)<br />
<ul><br />
<li>Junior, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine&nbsp;background&nbsp;</li><br />
<li>Interested in STS study</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/9/99/MJW.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:MJW.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/9/99/MJW.jpg" /></a></td><br />
<td><strong>Mr. Meng-Ju&nbsp;Wu</strong> (吳孟儒)<br />
<ul><br />
<li>Junior, Department of Life Sciences, National Yang Ming University</li><br />
<li>Life&nbsp;science background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/e/ee/ANL.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:ANL.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/e/ee/ANL.jpg" /></a></td><br />
<td><strong>Miss Yu-Hsien Lee</strong> (李宥嫺,<strong> Angel Lee</strong>)<br />
<ul><br />
<li>Sophomore, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/7/7b/WIC.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:WIC.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/7/7b/WIC.jpg" /></a></td><br />
<td><strong>Miss Shiang-Jiun Chen</strong> (陳香君,<strong> Wilma Chen</strong>)<br />
<ul><br />
<li>Sophomore, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/5/55/DML.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:DML.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/5/55/DML.jpg" /></a></td><br />
<td><strong>Mr. De-Ming&nbsp;Lin</strong> (林德銘)<br />
<ul><br />
<li>Sophomore, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine&nbsp;background</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/0/05/LHY.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:LHY.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/0/05/LHY.jpg" /></a></td><br />
<td><strong>Mr. Li-Hsiang Yen</strong> (顏立翔)<br />
<ul><br />
<li>Sophomore, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/7/74/MLL2.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:MLL2.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/7/74/MLL2.jpg" /></a></td><br />
<td><strong>Miss Min-Lun&nbsp;Li</strong> (李明倫)<br />
<ul><br />
<li>Freshman, Department of Life Sciences, National Yang Ming University</li><br />
<li>Life sciences&nbsp;background</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/1/11/CJY.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:CJY.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/1/11/CJY.jpg" /></a></td><br />
<td><strong>Miss Chun-Ju&nbsp;Yang</strong> (楊惇茹)<br />
<ul><br />
<li>Freshman, Department of Dentistry, National Yang Ming University</li><br />
<li>Dentistry&nbsp;background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
<hr /><br />
<p><br />
<table><br />
<tbody><br />
<tr><br />
<td><a class="image" title="" href="http://igem.ym.edu.tw/iGEM2007/images/f/f1/PCH.jpg"><img height="122" alt="" width="92" longdesc="/mediawiki/index.php/Image:PCH.jpg" src="http://igem.ym.edu.tw/iGEM2007/images/f/f1/PCH.jpg" /></a></td><br />
<td><strong>Mr. Po-Cheng&nbsp;Hsu</strong> (徐伯誠)<br />
<ul><br />
<li>Freshman, Department of Medicine, National Yang Ming University</li><br />
<li>Medicine&nbsp;background&nbsp;</li><br />
</ul><br />
</td><br />
</tr><br />
</tbody><br />
</table><br />
</p><br />
</html><br />
[https://2007.igem.org/Taipei Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:34:35Z
<p>Yfchang: </p>
<hr />
<div><table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
<ul><br />
<li><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!]</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Materials Materials]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Benchman Benchman]</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Costruct Construct]</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!]&nbsp;</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Paper Paper]&nbsp;</li> <br />
<li>[https://2007.igem.org/Taipei Back]</li> <br />
</ul><br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2"><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg</td><br />
</tr><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg</td><br />
</tr><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:32:37Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
<ul><br />
<li><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!]</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Materials Materials]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Benchman Benchman]</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Costruct Construct]</li> <br />
<li>[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!]&nbsp;</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back]</li> <br />
</ul><br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2"><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg</td><br />
</tr><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:31:28Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2"><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg</td><br />
</tr><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:30:29Z
<p>Yfchang: </p>
<hr />
<div><table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2"><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ <br />
National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg</td><br />
</tr><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:29:23Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2">[/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ <br />
National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg</td><br />
</tr><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:27:48Z
<p>Yfchang: </p>
<hr />
<div><br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2">[/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ <br />
National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg</td><br />
</tr><br />
<tr><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg</td><br />
<td>http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>[http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" width="300" height="352" /> [http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" width="300" height="388" />&nbsp;</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:27:00Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2">[/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ <br />
National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>[img:http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg]</td><br />
<td>[img:http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg]</td><br />
</tr><br />
<tr><br />
<td>[img:http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg]</td><br />
<td>[img:http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg]</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>[http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" width="300" height="352" /> [http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" width="300" height="388" />&nbsp;</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:26:09Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td valign="top" ><br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" color="#000000">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></li><br />
<li><font size="3" color="#000000">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><strong><font size="4" color="#993366">Our NYMU iGEM team meets regularly every week.<br /> <br />
</font> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font><br /> <br />
<font size="2">[/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ <br />
National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td>[http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg]</td><br />
<td>[http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg]</td><br />
</tr><br />
<tr><br />
<td>[http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg]</td><br />
<td>[http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg]</td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p>[http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" width="300" height="352" /> [http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" width="300" height="388" />&nbsp;</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:23:44Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td rowspan="2" valign="top"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td > <br />
<p> </p><br />
</td><br />
</tr><br />
<tr><br />
<td "> <br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference] are now available.<br /> <br />
<br /><br />
</font></font></li><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font></font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><br />
<font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html"><font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - [http://www.ym.edu.tw/ <br />
National Yang Ming University]<br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" width="320" height="225" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" width="320" height="225" /></td><br />
</tr><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" width="320" height="225" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" width="320" height="225" /></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" width="300" height="352" /> <img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" width="300" height="388" />&nbsp;</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:21:29Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td align="top" rowspan="2"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td > <br />
<p> </p><br />
</td><br />
</tr><br />
<tr><br />
<td "> <br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial"><a href="http://www.syntheticbiology3.ethz.ch/monday.htm">Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference</a> are now available.<br /> <br />
<br /><br />
</font></font></li><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the <a href="http://pub.ucsf.edu/today/cache/feature/200708022.html">UCSF Summer Assignment: Genetically Engineer a New Cell</a>.</font></font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><br />
<font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font><a href="http://www.ym.edu.tw/map.html"><font size="2">check map here!</font></a><font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - <a href="http://www.ym.edu.tw/">National Yang Ming University</a><br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" width="320" height="225" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" width="320" height="225" /></td><br />
</tr><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" width="320" height="225" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" width="320" height="225" /></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster<br /> <br />
</strong></font></font></p><br />
<p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" width="300" height="352" /> <img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" width="300" height="388" />&nbsp;</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:20:02Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td rowspan="2"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td > <br />
<p> </p><br />
</td><br />
</tr><br />
<tr><br />
<td "> <br />
<p><font size="4" color="#ff0000"><strong>Latest News<br /> <br />
</strong></font></p><br />
<ul><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial"><a href="http://www.syntheticbiology3.ethz.ch/monday.htm">Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference</a> are now available.<br /> <br />
<br /><br />
</font></font></li><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the <a href="http://pub.ucsf.edu/today/cache/feature/200708022.html">UCSF Summer Assignment: Genetically Engineer a New Cell</a>.</font></font>&nbsp;&nbsp;</li> <br />
</ul><br />
<ul><br /><br />
<li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /> <br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><br />
<font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /> <br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /> <br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /> <br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font><a href="http://www.ym.edu.tw/map.html"><font size="2">check map here!</font></a><font size="2"> We are at the P3 location on the map)</font>&nbsp;</li> <br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - <a href="http://www.ym.edu.tw/">National Yang Ming University</a><br /> <br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" width="320" height="225" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" width="320" height="225" /></td><br />
</tr><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" width="320" height="225" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" width="320" height="225" /></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster (<a href="http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg">download full size version</a>)<br /> <br />
</strong></font></font></p><br />
<p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" width="300" height="352" /> <img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" width="300" height="388" />&nbsp;</p> <br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:17:53Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td " rowspan="2"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper] <br />
<br />
[https://2007.igem.org/Taipei Back] <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
</td><br />
<td "> <br />
<p> </p><br />
</td><br />
</tr><br />
<tr><br />
<td "> <br />
<p> </p><br />
<br />
<p> </p><br />
<br />
<p> </p><br />
</td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:16:22Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td "><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]<br />
<br />
</td><br />
<td "> </td><br />
<td "> </td><br />
</tr><br />
<tr><br />
<td "> </td><br />
<td "> </td><br />
<td "> </td><br />
</tr><br />
<tr><br />
<td "> </td><br />
<td "> </td><br />
<td "> </td><br />
</tr><br />
<tr><br />
<td "> </td><br />
<td "> </td><br />
<td "> </td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:15:10Z
<p>Yfchang: </p>
<hr />
<div><br />
<br />
<table border="0" width="100%" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="33%"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]<br />
<br />
</td><br />
<td width="33%"> </td><br />
<td width="34%"> </td><br />
</tr><br />
<tr><br />
<td width="33%"> </td><br />
<td width="33%"> </td><br />
<td width="34%"> </td><br />
</tr><br />
<tr><br />
<td width="33%"> </td><br />
<td width="33%"> </td><br />
<td width="34%"> </td><br />
</tr><br />
<tr><br />
<td width="33%"> </td><br />
<td width="33%"> </td><br />
<td width="34%"> </td><br />
</tr><br />
</table></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:14:37Z
<p>Yfchang: </p>
<hr />
<div><div class="maincontent"><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]<br />
</div></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:13:19Z
<p>Yfchang: </p>
<hr />
<div><br />
[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-25T13:12:41Z
<p>Yfchang: </p>
<hr />
<div><html><br />
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[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]<br />
<br />
</td><br />
<td width="33%"> </td><br />
<td width="34%"> </td><br />
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Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Paper
NYMU Taipei/Paper
2007-10-24T15:37:49Z
<p>Yfchang: </p>
<hr />
<div><p><font color="#000000" size="2">A very expensive book on Synthetic Biology (US$2700 for single user license edition):</font></p><br />
<p><font color="#000000" size="3">[http://www.bioportfolio.com/cgi-bin/acatalog/Synthetic_Biology__A_New_Paradigm_for_Biological_D.html Synthetic Biology: A New Paradigm for Biological Discovery]</font></p><br />
<hr /><br />
<p><font color="#0000ff" size="3">Sorted by publication date in descending order</font></p><br />
<p><font size="2">A bottom-up approach to gene regulation.<br /><br />
Guido NJ, Wang X, Adalsteinsson D, McMillen D, Hasty J, Cantor CR, Elston TC, Collins JJ<br /><br />
Nature(30.979) , 2006 - USA(Massachusetts)</font></p><br />
<p><font size="2">Regulation of flagella.<br /><br />
McCarter LL<br /><br />
Curr Opin Microbiol(6.869) , 2006 - USA(Iowa)</font></p><br />
<p><font size="2">Regulatory dynamics of synthetic gene networks with positive feedback.<br /><br />
Maeda YT, Sano M<br /><br />
J Mol Biol(5.239) , 2006 - Japan</font></p><br />
<p><font size="2">Synthetic biology: new engineering rules for an emerging discipline.<br /><br />
Andrianantoandro E, Basu S, Karig DK, Weiss R<br /><br />
Mol Syst Biol(0) , 2006 - USA(New Jersey)</font></p><br />
<p><font size="2">A synthetic multicellular system for programmed pattern formation.<br /><br />
Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R<br /><br />
Nature(30.979) , 2005 - USA(New Jersey)</font></p><br />
<p><font size="2">A synthetic gene-metabolic oscillator.<br /><br />
Fung E, Wong WW, Suen JK, Bulter T, Lee SG, Liao JC<br /><br />
Nature(30.979) , 2005 - USA(California)</font></p><br />
<p><font size="2">Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.<br /><br />
Balagadde FK, You L, Hansen CL, Arnold FH, Quake SR<br /><br />
Science(29.162) , 2005 - USA(California)</font></p><br />
<p><font size="2">Artificial cell-cell communication in yeast Saccharomyces cerevisiae using signaling elements from Arabidopsis thaliana.<br /><br />
Chen MT, Weiss R<br /><br />
Nat Biotechnol(17.721) , 2005 - USA(New Jersey)</font></p><br />
<p><font size="2">Hysteresis in a synthetic mammalian gene network.<br /><br />
Kramer BP, Fussenegger M<br /><br />
Proc Natl Acad Sci U S A(10.272) , 2005 - Switzerland</font></p><br />
<p><font size="2">Ultrasensitivity and noise propagation in a synthetic transcriptional cascade.<br /><br />
Hooshangi S, Thiberge S, Weiss R<br /><br />
Proc Natl Acad Sci U S A(10.272) , 2005 - USA(New Jersey)</font></p><br />
<p><font size="2">Semi-synthetic mammalian gene regulatory networks.<br /><br />
Kramer BP, Fischer M, Fussenegger M<br /><br />
Metab Eng(3.397) , 2005 - Switzerland</font></p><br />
<p><font size="2">Transcriptional regulation and metabolism.<br /><br />
Brynildsen MP, Wong WW, Liao JC<br /><br />
Biochem Soc Trans(2.579) , 2005 - USA(California)</font></p><br />
<p><font size="2">Signal-amplifying genetic circuit enables in vivo observation of weak promoter activation in the Rhl quorum sensing system.<br /><br />
Karig DK, Weiss R<br /><br />
Biotechnol Bioeng(2.173) , 2005 - USA(New Jersey)</font></p><br />
<p><font size="2">A network of orthogonal ribosome x mRNA pairs.<br /><br />
Rackham O, Chin JW<br /><br />
Nat Chem Biol(0) , 2005 - UK</font></p><br />
<p><font size="2">Programmed population control by cell-cell communication and regulated killing.<br /><br />
You L, Cox RS 3rd, Weiss R, Arnold FH<br /><br />
Nature(30.979) , 2004 - USA(California)</font></p><br />
<p><font size="2">Using a quantitative blueprint to reprogram the dynamics of the flagella gene network.<br /><br />
Kalir S, Alon U<br /><br />
Cell(26.626) , 2004 - Israel</font></p><br />
<p><font size="2">An engineered epigenetic transgene switch in mammalian cells.<br /><br />
Kramer BP, Viretta AU, Daoud-El-Baba M, Aubel D, Weber W, Fussenegger M<br /><br />
Nat Biotechnol(17.721) , 2004 - Switzerland</font></p><br />
<p><font size="2">Programmable cells: interfacing natural and engineered gene networks.<br /><br />
Kobayashi H, Kaern M, Araki M, Chung K, Gardner TS, Cantor CR, Collins JJ<br /><br />
Proc Natl Acad Sci U S A(10.272) , 2004 - USA(Massachusetts)</font></p><br />
<p><font size="2">Design of artificial cell-cell communication using gene and metabolic networks.<br /><br />
Bulter T, Lee SG, Wong WW, Fung E, Connor MR, Liao JC<br /><br />
Proc Natl Acad Sci U S A(10.272) , 2004 - USA(California)</font></p><br />
<p><font size="2">Spatiotemporal control of gene expression with pulse-generating networks.<br /><br />
Basu S, Mehreja R, Thiberge S, Chen MT, Weiss R<br /><br />
Proc Natl Acad Sci U S A(10.272) , 2004 - USA(New Jersey)</font></p><br />
<p><font size="2">Optimizing genetic circuits by global sensitivity analysis.<br /><br />
Feng XJ, Hooshangi S, Chen D, Li G, Weiss R, Rabitz H<br /><br />
Biophys J(4.463) , 2004 - USA(New Jersey)</font></p><br />
<p><font size="2">Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli.<br /><br />
Atkinson MR, Savageau MA, Myers JT, Ninfa AJ<br /><br />
Cell(26.626) , 2003 - USA(Michigan)</font></p><br />
<p><font size="2">Combinatorial synthesis of genetic networks.<br /><br />
Guet CC, Elowitz MB, Hsing W, Leibler S<br /><br />
Science(29.162) , 2002 - USA(New Jersey)</font></p><br />
<p><font size="2">Directed evolution of a genetic circuit.<br /><br />
Yokobayashi Y, Weiss R, Arnold FH<br /><br />
Proc Natl Acad Sci U S A(10.272) , 2002 - USA(California)</font></p><br />
<p><font size="2">Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion.<br /><br />
Becskei A, Seraphin B, Serrano L<br /><br />
EMBO J(10.456) , 2001 - Germany</font></p><br />
<p><font size="2">A synthetic oscillatory network of transcriptional regulators.<br /><br />
Elowitz MB, Leibler S<br /><br />
Nature(30.979) , 2000 - USA(New Jersey)</font></p><br />
<p><font size="2">Engineering stability in gene networks by autoregulation.<br /><br />
Becskei A, Serrano L<br /><br />
Nature(30.979) , 2000 - Germany</font></p><br />
<p><font size="2">Construction of a genetic toggle switch in Escherichia coli.<br /><br />
Gardner TS, Cantor CR, Collins JJ<br /><br />
Nature(30.979) , 2000 - USA(Massachusetts)</font></p><br />
<p>&nbsp;</p><br />
<hr /><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/You_must_know!
NYMU Taipei/You must know!
2007-10-24T15:36:51Z
<p>Yfchang: </p>
<hr />
<div><ul><br />
<li><font size="2">[https://2007.igem.org/NYMU_Taipei/You_must_know!/names_&amp;_peoples_you_should_know names &amp; peoples you should know]</font></li><br />
<li>[http://140.119.115.32/sa/chenread.asp?docsn=2006072815&amp;readtype=ce DNA元件,組裝未來生命(中英文對照導讀)]</li><br />
<li>[https://2007.igem.org/Jamboree <font size="2> iGEM 2007 jamboree</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Protocols <font size="2> Protocols from OpenWetware</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Laboratory_Fundamentals_of_Synthetic_Biology <font size="2">Laboratory Fundamentals of Synthetic Biology</font>]<font size="2">&nbsp; </font></li><br />
<li>[http://www.evolvingcode.net/codon/ <font size="2">Synthetic Gene Design Software</font>]<font size="2"> </font></li><br />
<li>[http://www.genome.ad.jp/kegg/pathway/eco/eco02020.html <font size="2">Two-component system</font>]<font size="2"> </font><br />
<ul><br />
<li>[http://igem.ym.edu.tw/private_2007/images/e/e0/Eco02020.gif <font size="2"><em>Escherichia coli</em> K-12 MG1655</font>]<font size="2"> </font></li><br />
<li>[http://www.bmb.psu.edu/nixon/micro401/overview.htm <font size="2">Two Component Signal Transduction-Controlled ATP Hydrolysis as a Second Messenger</font>]<font size="2">&nbsp; </font></li><br />
</ul><br />
</li><br />
<li>[http://en.wikipedia.org/wiki/Quorum_sensing <font size="2">Quorum sensing</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Naming_a_new_biological_part <font size="2">BioBrick nomenclature</font>]<font size="2"> </font></li><br />
<li>[http://partsregistry.org/Help:Plasmids/Nomenclature <font size="2">Plasmid nomenclature</font>]</li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#vectors Plasmid&nbsp;vectors]</font></li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-DNA E. coli strains for DNA propagation and cloning]</font></li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-exp E. coli strains for protein expression]</font></li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155807 E. coli Host Strain Genetic Markers]</font></li><br />
<li><font size="2">[http://www.clontech.com/upload/images/tools/Molecular%20Biology%20Tools/E.%20coli%20Host%20Strain%20Genotypes.pdf <em>E. coli</em> Host Strain Genotypes]</font></li><br />
<li>[http://partsregistry.org/partsdb/pgroup.cgi?pgroup=cell <font size="2">E. coli strain</font>]<font size="2"> </font><br />
<ul><br />
<li><font size="2">[http://openwetware.org/wiki/E._coli_genotypes The description of E.coli strains on OpenWetware]</font></li><br />
</ul><br />
</li><br />
<li><font size="2">[http://www.neb.com/nebecomm/tech_reference/default.asp Technical Reference] (from New England Biolabs; NEB)</font></li><br />
<li>[http://www.merckbio.com.tw/promotion_b.asp?saleid=000004&amp;rnd=0.7747401 PfuUltra II]</li><br />
<li>[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155809 <font size="2">The Genetic Code</font>]</li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155810 Molecular biology common conversions]</font></li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155811 Gel Electrophoresis Guidelines]</font></li><br />
<li>[http://www.clontech.com/default.asp Clontech]</li><br />
<li>Novagen</li><br />
<li>Invitrogen</li><br />
<li>Stratagene</li><br />
<li><font size="2">[http://www.insilico.uni-duesseldorf.de/Lig_Input.html Ligation Calculator]</font></li><br />
<li><font size="2">[http://www.promega.com/biomath/default.htm BioMath Calculators]</font></li><br />
<li><font size="2">[http://www.biocompare.com/jump/2045/Antibody-Search.html Antibody Search]</font></li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/You_must_know!
NYMU Taipei/You must know!
2007-10-24T15:24:24Z
<p>Yfchang: </p>
<hr />
<div><ul><br />
<li><font size="2">[https://2007.igem.org/NYMU_Taipei/You_must_know!/names_&amp;_peoples_you_should_know names &amp; peoples you should know]</font></li><br />
<li>[http://140.119.115.32/sa/chenread.asp?docsn=2006072815&amp;readtype=ce DNA元件,組裝未來生命(中英文對照導讀)]</li><br />
<li>[https://2007.igem.org/Jamboree <font size="2 iGEM 2007 jamboree</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Protocols <font size="2 Protocols from OpenWetware</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Laboratory_Fundamentals_of_Synthetic_Biology <font size="2">Laboratory Fundamentals of Synthetic Biology</font>]<font size="2">&nbsp; </font></li><br />
<li>[http://www.evolvingcode.net/codon/ <font size="2">Synthetic Gene Design Software</font>]<font size="2"> </font></li><br />
<li>[http://www.genome.ad.jp/kegg/pathway/eco/eco02020.html <font size="2">Two-component system</font>]<font size="2"> </font><br />
<ul><br />
<li>[http://igem.ym.edu.tw/private_2007/images/e/e0/Eco02020.gif <font size="2"><em>Escherichia coli</em> K-12 MG1655</font>]<font size="2"> </font></li><br />
<li>[http://www.bmb.psu.edu/nixon/micro401/overview.htm <font size="2">Two Component Signal Transduction-Controlled ATP Hydrolysis as a Second Messenger</font>]<font size="2">&nbsp; </font></li><br />
</ul><br />
</li><br />
<li>[http://en.wikipedia.org/wiki/Quorum_sensing <font size="2">Quorum sensing</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Naming_a_new_biological_part <font size="2">BioBrick nomenclature</font>]<font size="2"> </font></li><br />
<li>[http://partsregistry.org/Help:Plasmids/Nomenclature <font size="2">Plasmid nomenclature</font>]</li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#vectors Plasmid&nbsp;vectors]</font></li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-DNA E. coli strains for DNA propagation and cloning]</font></li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-exp E. coli strains for protein expression]</font></li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155807 E. coli Host Strain Genetic Markers]</font></li><br />
<li><font size="2">[http://www.clontech.com/upload/images/tools/Molecular%20Biology%20Tools/E.%20coli%20Host%20Strain%20Genotypes.pdf <em>E. coli</em> Host Strain Genotypes]</font></li><br />
<li>[http://partsregistry.org/partsdb/pgroup.cgi?pgroup=cell <font size="2">E. coli strain</font>]<font size="2"> </font><br />
<ul><br />
<li><font size="2">[http://openwetware.org/wiki/E._coli_genotypes The description of E.coli strains on OpenWetware]</font></li><br />
</ul><br />
</li><br />
<li><font size="2">[http://www.neb.com/nebecomm/tech_reference/default.asp Technical Reference] (from New England Biolabs; NEB)</font></li><br />
<li>[http://www.merckbio.com.tw/promotion_b.asp?saleid=000004&amp;rnd=0.7747401 PfuUltra II]</li><br />
<li>[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155809 <font size="2">The Genetic Code</font>]</li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155810 Molecular biology common conversions]</font></li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155811 Gel Electrophoresis Guidelines]</font></li><br />
<li>[http://www.clontech.com/default.asp Clontech]</li><br />
<li>Novagen</li><br />
<li>Invitrogen</li><br />
<li>Stratagene</li><br />
<li><font size="2">[http://www.insilico.uni-duesseldorf.de/Lig_Input.html Ligation Calculator]</font></li><br />
<li><font size="2">[http://www.promega.com/biomath/default.htm BioMath Calculators]</font></li><br />
<li><font size="2">[http://www.biocompare.com/jump/2045/Antibody-Search.html Antibody Search]</font></li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/You_must_know!
NYMU Taipei/You must know!
2007-10-24T15:24:05Z
<p>Yfchang: </p>
<hr />
<div><ul><br />
<li><font size="2">[https://2007.igem.org/NYMU_Taipei/You_must_know!/names_&amp;_peoples_you_should_know names &amp; peoples you should know]</font></li><br />
<li>[http://140.119.115.32/sa/chenread.asp?docsn=2006072815&amp;readtype=ce DNA元件,組裝未來生命(中英文對照導讀)]</li><br />
<li>[https://2007.igem.org/Jamboree"><font size="2 iGEM 2007 jamboree</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Protocols"><font size="2 Protocols from OpenWetware</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Laboratory_Fundamentals_of_Synthetic_Biology <font size="2">Laboratory Fundamentals of Synthetic Biology</font>]<font size="2">&nbsp; </font></li><br />
<li>[http://www.evolvingcode.net/codon/ <font size="2">Synthetic Gene Design Software</font>]<font size="2"> </font></li><br />
<li>[http://www.genome.ad.jp/kegg/pathway/eco/eco02020.html <font size="2">Two-component system</font>]<font size="2"> </font><br />
<ul><br />
<li>[http://igem.ym.edu.tw/private_2007/images/e/e0/Eco02020.gif <font size="2"><em>Escherichia coli</em> K-12 MG1655</font>]<font size="2"> </font></li><br />
<li>[http://www.bmb.psu.edu/nixon/micro401/overview.htm <font size="2">Two Component Signal Transduction-Controlled ATP Hydrolysis as a Second Messenger</font>]<font size="2">&nbsp; </font></li><br />
</ul><br />
</li><br />
<li>[http://en.wikipedia.org/wiki/Quorum_sensing <font size="2">Quorum sensing</font>]<font size="2"> </font></li><br />
<li>[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Naming_a_new_biological_part <font size="2">BioBrick nomenclature</font>]<font size="2"> </font></li><br />
<li>[http://partsregistry.org/Help:Plasmids/Nomenclature <font size="2">Plasmid nomenclature</font>]</li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#vectors Plasmid&nbsp;vectors]</font></li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-DNA E. coli strains for DNA propagation and cloning]</font></li><br />
<li><font size="2">[http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-exp E. coli strains for protein expression]</font></li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155807 E. coli Host Strain Genetic Markers]</font></li><br />
<li><font size="2">[http://www.clontech.com/upload/images/tools/Molecular%20Biology%20Tools/E.%20coli%20Host%20Strain%20Genotypes.pdf <em>E. coli</em> Host Strain Genotypes]</font></li><br />
<li>[http://partsregistry.org/partsdb/pgroup.cgi?pgroup=cell <font size="2">E. coli strain</font>]<font size="2"> </font><br />
<ul><br />
<li><font size="2">[http://openwetware.org/wiki/E._coli_genotypes The description of E.coli strains on OpenWetware]</font></li><br />
</ul><br />
</li><br />
<li><font size="2">[http://www.neb.com/nebecomm/tech_reference/default.asp Technical Reference] (from New England Biolabs; NEB)</font></li><br />
<li>[http://www.merckbio.com.tw/promotion_b.asp?saleid=000004&amp;rnd=0.7747401 PfuUltra II]</li><br />
<li>[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155809 <font size="2">The Genetic Code</font>]</li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155810 Molecular biology common conversions]</font></li><br />
<li><font size="2">[http://www.clontech.com/support/tools.asp?product_tool_id=155808&amp;tool_id=155811 Gel Electrophoresis Guidelines]</font></li><br />
<li>[http://www.clontech.com/default.asp Clontech]</li><br />
<li>Novagen</li><br />
<li>Invitrogen</li><br />
<li>Stratagene</li><br />
<li><font size="2">[http://www.insilico.uni-duesseldorf.de/Lig_Input.html Ligation Calculator]</font></li><br />
<li><font size="2">[http://www.promega.com/biomath/default.htm BioMath Calculators]</font></li><br />
<li><font size="2">[http://www.biocompare.com/jump/2045/Antibody-Search.html Antibody Search]</font></li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Benchman
NYMU Taipei/Benchman
2007-10-24T14:50:56Z
<p>Yfchang: </p>
<hr />
<div><h3>OD and GEL checks</h3><br />
<table width="100%" align="center" border="1"><br />
<br />
<tr align="center"><br />
<td><font size="1"><strong>Part name<br /><br />
</strong></font></td><br />
<td><font size="1"><strong>Type and Description<br /><br />
</strong></font></td><br />
<td><font size="1"><strong>length (bp) </strong></font></td><br />
<td><font size="1"><strong>PCR&nbsp; check</strong></font></td><br />
<td><font size="1"><strong>weight (g) </strong></font></td><br />
<td><font size="1"><strong>concentration (ug/uL) </strong></font></td><br />
<td><font size="1"><strong>A260/A280</strong></font></td><br />
<td><font size="1"><strong>A260/A230</strong></font></td><br />
<td><font size="1"><strong>date</strong></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #1)</font></td><br />
<td align="left"><font size="1">plasmid extraction #1</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.20</font></td><br />
<td align="right"><font size="1">1.30</font></td><br />
<td align="right"><font size="1">0.97</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #2)</font></td><br />
<td align="left"><font size="1">plasmid extraction #2</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.19</font></td><br />
<td align="right"><font size="1">1.33</font></td><br />
<td align="right"><font size="1">1.00</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #3)</font></td><br />
<td align="left"><font size="1">plasmid extraction #3</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.21</font></td><br />
<td align="right"><font size="1">1.42</font></td><br />
<td align="right"><font size="1">1.12</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #4)</font></td><br />
<td align="left"><font size="1">plasmid extraction #4</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.25</font></td><br />
<td align="right"><font size="1">1.37</font></td><br />
<td align="right"><font size="1">1.03</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (D. SP)</font></td><br />
<td align="left"><font size="1">digestion (S. P.) as back vector, #4 selected</font></td><br />
<td align="right"><font size="1">2,253</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.14</font></td><br />
<td align="right"><font size="1">0.01</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4 Oct 04, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATA_INSA (D. XP)</font></td><br />
<td align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td align="right"><font size="1">&nbsp;193</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.25</font></td><br />
<td align="right"><font size="1">0.02</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">0.29</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATA_INSA (PCR)</font></td><br />
<td align="left"><font size="1">PCR for TATA + INSA</font></td><br />
<td align="right"><font size="1">209</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.24; 0.30</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATB_INSB (PCR)</font></td><br />
<td align="left"><font size="1">PCR for TATB + INSB</font></td><br />
<td align="right"><font size="1">233</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.35; 0.35</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATA_INSA (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;193</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_10 Oct 10, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATB_INSB (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">220</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_10Oct 10, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pOmpC (D. SP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong>, #1 selected</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2,253</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC + TATA_INSA</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">back ligation pOmpC (D. SP) + TATA_INSA (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;2,454</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1"><font color="#ff0000">430</font>, 613</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">Oct 13, 07 &amp; Oct 16,07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC+TATA_INSA (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,436 2,427</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC+TATA_INSA + TATB_INSB</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">back ligation pOmpC+TATA_INSA (D. SP) + TATB_INSB (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,655</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font color="#ff0000" size="1">613</font><font size="1">, 814</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATB_INSB (D. ES)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (E. S.) as front insert (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.27</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.03</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.21</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (P.E.)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">plasmid extraction #1, #3, #4 (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.41, 0.25, 0.41</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_27#CinR.2BHSL.2BD-term_check">Sep 27, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (D. E)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">first digestion (E.) as front vector (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.19</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (D. EX)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">second digestion (X.) as front vector (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.19</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.02</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.13</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4 Oct 04, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATB_INSB + CinR+HSL+D-term</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">front ligation TATB_INSB (D. ES) + CinR+HSL+D-term (D. EX) (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 05, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="center"><font size="1">pOmpC+TATA_INSA+TATB_INSB (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S.P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,637</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="center"><font size="1">CinR+HSL+D-term (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X.P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">1,647</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="center"><font size="1"><strong>system 1</strong></font></td><br />
<td align="left"><font size="1">ligation pOmpC+TATA_INSA+TATB_INSB + CinR+HSL+D-term</font></td><br />
<td align="right"><font size="1">4,292</font></td><br />
<td align="right"><font color="#ff0000" size="1">814</font><font size="1">, 2,451</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (P.E. #1)</font></td><br />
<td align="left"><font size="1">plasmid extraction #1</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.23</font></td><br />
<td align="right"><font size="1">1.70</font></td><br />
<td align="right"><font size="1">1.49</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (P.E. #2)</font></td><br />
<td align="left"><font size="1">plasmid extraction #2</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.29</font></td><br />
<td align="right"><font size="1">1.58</font></td><br />
<td align="right"><font size="1">1.26</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (D. SP)</font></td><br />
<td align="left"><font size="1">digestion (S. P.) as back vector, #1 selected</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.17</font></td><br />
<td align="right"><font size="1">0.03</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">0.18</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4 Oct 04, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">OmpRBS (D. XP)</font></td><br />
<td align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.24</font></td><br />
<td align="right"><font size="1">0.01</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">ligation pCinRHSL (D. SP) + OmpRBS (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 05, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as back vector (a bit smear)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">2,443</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.14 (too few)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS (plasmid)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">plasmid extraction of pCinRHSL+OmpRBS (clone 7 and clone 10 on the plate) after liquid culture</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.020; 0.019</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">1.95; 1.83</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2.18; 2.31</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as back vector (smear)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;2,443</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as <strong>back vector</strong> using plasmid from clone 7</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2,443</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.05</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.22</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATD_IDE (D. XP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">3,190</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.10 (too few)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATD + IDE (PCR)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">re-PCR for TATD + IDE</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">3,203</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">TATD_IDE (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;3,190</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pCinRHSL+OmpRBS + TATD_IDE</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">ligation pCinRHSL+OmpRBS + TATD_IDE</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pCinRHSL+OmpRBS+TATD_IDE (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">D-term (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="center"><font size="1"><strong>system 2</strong></font></td><br />
<td align="left"><font size="1">ligation pCinRHSL+OmpRBS+TATD_IDE + D-term</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td><font size="1">&nbsp;</font></td><br />
<td><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<br />
</table><br />
<hr /><br />
<br />
<h3>Notes</h3><br />
<ul><br />
<li>A260/A280 should be around 1.70</li><br />
<li>A260/A230 should be less than 3.00</li><br />
<li>color key<br />
<ul><br />
<li>problemistic (read)</li><br />
<li>finished (yellow)</li><br />
<li>await (blue)</li><br />
</ul><br />
</li><br />
<li>digestion and buffer<br />
<ul><br />
<li>SepI and PstI (<strong>SP</strong>) digestion using buffer <strong>2</strong>, creates back <strong>vector</strong></li><br />
<li>XbaI and PstI (<strong>XP</strong>) digestion using buffer <strong>3</strong>, creates back <strong>insert</strong></li><br />
</ul><br />
</li><br />
<li>Assembly process<br />
<ul><br />
<li><strong>STEP 1</strong>. prepare <strong>insert and vector</strong> by <strong>digestion</strong></li><br />
<li><strong>STEP 2</strong>. check digestion by GEL separation<br />
<ul><br />
<li>if band is clear at correct position (length), cut the gel and purify/extract it</li><br />
<li>else go back to STEP 1.</li><br />
</ul><br />
</li><br />
<li><strong>STEP 3</strong>. <strong>ligate </strong>insert with vector, <strong>transform </strong>it into competent cell, and extract plasmid from liquid culture</li><br />
<li><strong>STEP 4</strong>. check ligation by <strong>VF2, VR PCR</strong> with GEL separation<br />
<ul><br />
<li>if band is clear at correct position (length), cut the gel and purify/extract it (ligation is done)</li><br />
<li>else go back to STEP 3.</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Benchman
NYMU Taipei/Benchman
2007-10-24T14:50:08Z
<p>Yfchang: </p>
<hr />
<div><h3>OD and GEL checks</h3><br />
<table width="100%" align="center" border="1"><br />
<br />
<tr align="center"><br />
<td><font size="1"><strong>Part name<br /><br />
</strong></font></td><br />
<td><font size="1"><strong>Type and Description<br /><br />
</strong></font></td><br />
<td><font size="1"><strong>length (bp) </strong></font></td><br />
<td><font size="1"><strong>PCR&nbsp; check</strong></font></td><br />
<td><font size="1"><strong>weight (g) </strong></font></td><br />
<td><font size="1"><strong>concentration (ug/uL) </strong></font></td><br />
<td><font size="1"><strong>A260/A280</strong></font></td><br />
<td><font size="1"><strong>A260/A230</strong></font></td><br />
<td><font size="1"><strong>date</strong></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #1)</font></td><br />
<td align="left"><font size="1">plasmid extraction #1</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.20</font></td><br />
<td align="right"><font size="1">1.30</font></td><br />
<td align="right"><font size="1">0.97</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #2)</font></td><br />
<td align="left"><font size="1">plasmid extraction #2</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.19</font></td><br />
<td align="right"><font size="1">1.33</font></td><br />
<td align="right"><font size="1">1.00</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #3)</font></td><br />
<td align="left"><font size="1">plasmid extraction #3</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.21</font></td><br />
<td align="right"><font size="1">1.42</font></td><br />
<td align="right"><font size="1">1.12</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #4)</font></td><br />
<td align="left"><font size="1">plasmid extraction #4</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.25</font></td><br />
<td align="right"><font size="1">1.37</font></td><br />
<td align="right"><font size="1">1.03</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (D. SP)</font></td><br />
<td align="left"><font size="1">digestion (S. P.) as back vector, #4 selected</font></td><br />
<td align="right"><font size="1">2,253</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.14</font></td><br />
<td align="right"><font size="1">0.01</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4 Oct 04, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATA_INSA (D. XP)</font></td><br />
<td align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td align="right"><font size="1">&nbsp;193</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.25</font></td><br />
<td align="right"><font size="1">0.02</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">0.29</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATA_INSA (PCR)</font></td><br />
<td align="left"><font size="1">PCR for TATA + INSA</font></td><br />
<td align="right"><font size="1">209</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.24; 0.30</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATB_INSB (PCR)</font></td><br />
<td align="left"><font size="1">PCR for TATB + INSB</font></td><br />
<td align="right"><font size="1">233</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.35; 0.35</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATA_INSA (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;193</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_10 Oct 10, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATB_INSB (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">220</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_10Oct 10, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pOmpC (D. SP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong>, #1 selected</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2,253</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC + TATA_INSA</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">back ligation pOmpC (D. SP) + TATA_INSA (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;2,454</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1"><font color="#ff0000">430</font>, 613</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">Oct 13, 07 &amp; Oct 16,07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC+TATA_INSA (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,436 2,427</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC+TATA_INSA + TATB_INSB</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">back ligation pOmpC+TATA_INSA (D. SP) + TATB_INSB (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,655</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font color="#ff0000" size="1">613</font><font size="1">, 814</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATB_INSB (D. ES)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (E. S.) as front insert (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.27</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.03</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.21</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (P.E.)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">plasmid extraction #1, #3, #4 (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.41, 0.25, 0.41</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_27#CinR.2BHSL.2BD-term_check">Sep 27, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (D. E)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">first digestion (E.) as front vector (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.19</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (D. EX)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">second digestion (X.) as front vector (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.19</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.02</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.13</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4 Oct 04, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATB_INSB + CinR+HSL+D-term</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">front ligation TATB_INSB (D. ES) + CinR+HSL+D-term (D. EX) (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 05, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="center"><font size="1">pOmpC+TATA_INSA+TATB_INSB (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S.P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,637</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="center"><font size="1">CinR+HSL+D-term (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X.P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">1,647</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="center"><font size="1"><strong>system 1</strong></font></td><br />
<td align="left"><font size="1">ligation pOmpC+TATA_INSA+TATB_INSB + CinR+HSL+D-term</font></td><br />
<td align="right"><font size="1">4,292</font></td><br />
<td align="right"><font color="#ff0000" size="1">814</font><font size="1">, 2,451</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (P.E. #1)</font></td><br />
<td align="left"><font size="1">plasmid extraction #1</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.23</font></td><br />
<td align="right"><font size="1">1.70</font></td><br />
<td align="right"><font size="1">1.49</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (P.E. #2)</font></td><br />
<td align="left"><font size="1">plasmid extraction #2</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.29</font></td><br />
<td align="right"><font size="1">1.58</font></td><br />
<td align="right"><font size="1">1.26</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (D. SP)</font></td><br />
<td align="left"><font size="1">digestion (S. P.) as back vector, #1 selected</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.17</font></td><br />
<td align="right"><font size="1">0.03</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">0.18</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4 Oct 04, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">OmpRBS (D. XP)</font></td><br />
<td align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.24</font></td><br />
<td align="right"><font size="1">0.01</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">ligation pCinRHSL (D. SP) + OmpRBS (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 05, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as back vector (a bit smear)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">2,443</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.14 (too few)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS (plasmid)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">plasmid extraction of pCinRHSL+OmpRBS (clone 7 and clone 10 on the plate) after liquid culture</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.020; 0.019</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">1.95; 1.83</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2.18; 2.31</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as back vector (smear)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;2,443</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as <strong>back vector</strong> using plasmid from clone 7</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2,443</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.05</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.22</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATD_IDE (D. XP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">3,190</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.10 (too few)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATD + IDE (PCR)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">re-PCR for TATD + IDE</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">3,203</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">TATD_IDE (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;3,190</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pCinRHSL+OmpRBS + TATD_IDE</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">ligation pCinRHSL+OmpRBS + TATD_IDE</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pCinRHSL+OmpRBS+TATD_IDE (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">D-term (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="center"><font size="1"><strong>system 2</strong></font></td><br />
<td align="left"><font size="1">ligation pCinRHSL+OmpRBS+TATD_IDE + D-term</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td><font size="1">&nbsp;</font></td><br />
<td><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<br />
</table><br />
<hr /><br />
<br />
<h3>Notes</h3><br />
<ul><br />
<li>A260/A280 should be around 1.70</li><br />
<li>A260/A230 should be less than 3.00</li><br />
<li>color key<br />
<ul><br />
<li>problemistic (read)</li><br />
<li>finished (yellow)</li><br />
<li>await (blue)</li><br />
</ul><br />
</li><br />
<li>digestion and buffer<br />
<ul><br />
<li>SepI and PstI (<strong>SP</strong>) digestion using buffer <strong>2</strong>, creates back <strong>vector</strong></li><br />
<li>XbaI and PstI (<strong>XP</strong>) digestion using buffer <strong>3</strong>, creates back <strong>insert</strong></li><br />
</ul><br />
</li><br />
<li>Assembly process<br />
<ul><br />
<li><strong>STEP 1</strong>. prepare <strong>insert and vector</strong> by <strong>digestion</strong></li><br />
<li><strong>STEP 2</strong>. check digestion by GEL separation<br />
<ul><br />
<li>if band is clear at correct position (length), cut the gel and purify/extract it</li><br />
<li>else go back to STEP 1.</li><br />
</ul><br />
</li><br />
<li><strong>STEP 3</strong>. <strong>ligate </strong>insert with vector, <strong>transform </strong>it into competent cell, and extract plasmid from liquid culture</li><br />
<li><strong>STEP 4</strong>. check ligation by <strong>VF2, VR PCR</strong> with GEL separation<br />
<ul><br />
<li>if band is clear at correct position (length), cut the gel and purify/extract it (ligation is done)</li><br />
<li>else go back to STEP 3.</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Benchman
NYMU Taipei/Benchman
2007-10-24T14:46:04Z
<p>Yfchang: </p>
<hr />
<div><h3>OD and GEL checks</h3><br />
<table width="100%" align="center" border="1"><br />
<tbody><br />
<tr align="center"><br />
<td><font size="1"><strong>Part name<br /><br />
</strong></font></td><br />
<td><font size="1"><strong>Type and Description<br /><br />
</strong></font></td><br />
<td><font size="1"><strong>length (bp) </strong></font></td><br />
<td><font size="1"><strong>PCR&nbsp; check</strong></font></td><br />
<td><font size="1"><strong>weight (g) </strong></font></td><br />
<td><font size="1"><strong>concentration (ug/uL) </strong></font></td><br />
<td><font size="1"><strong>A260/A280</strong></font></td><br />
<td><font size="1"><strong>A260/A230</strong></font></td><br />
<td><font size="1"><strong>date</strong></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #1)</font></td><br />
<td align="left"><font size="1">plasmid extraction #1</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.20</font></td><br />
<td align="right"><font size="1">1.30</font></td><br />
<td align="right"><font size="1">0.97</font></td><br />
<td align="right"><font size="1">[https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3 Oct 03, 07]</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #2)</font></td><br />
<td align="left"><font size="1">plasmid extraction #2</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.19</font></td><br />
<td align="right"><font size="1">1.33</font></td><br />
<td align="right"><font size="1">1.00</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #3)</font></td><br />
<td align="left"><font size="1">plasmid extraction #3</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.21</font></td><br />
<td align="right"><font size="1">1.42</font></td><br />
<td align="right"><font size="1">1.12</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (P.E. #4)</font></td><br />
<td align="left"><font size="1">plasmid extraction #4</font></td><br />
<td align="right"><font size="1">2,271</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.25</font></td><br />
<td align="right"><font size="1">1.37</font></td><br />
<td align="right"><font size="1">1.03</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pOmpC (D. SP)</font></td><br />
<td align="left"><font size="1">digestion (S. P.) as back vector, #4 selected</font></td><br />
<td align="right"><font size="1">2,253</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.14</font></td><br />
<td align="right"><font size="1">0.01</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4">Oct 04, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATA_INSA (D. XP)</font></td><br />
<td align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td align="right"><font size="1">&nbsp;193</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.25</font></td><br />
<td align="right"><font size="1">0.02</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">0.29</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATA_INSA (PCR)</font></td><br />
<td align="left"><font size="1">PCR for TATA + INSA</font></td><br />
<td align="right"><font size="1">209</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.24; 0.30</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">TATB_INSB (PCR)</font></td><br />
<td align="left"><font size="1">PCR for TATB + INSB</font></td><br />
<td align="right"><font size="1">233</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.35; 0.35</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATA_INSA (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;193</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_10">Oct 10, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATB_INSB (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">220</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_10">Oct 10, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pOmpC (D. SP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong>, #1 selected</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2,253</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC + TATA_INSA</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">back ligation pOmpC (D. SP) + TATA_INSA (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;2,454</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1"><font color="#ff0000">430</font>, 613</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">Oct 13, 07 &amp; Oct 16,07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC+TATA_INSA (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,436 2,427</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pOmpC+TATA_INSA + TATB_INSB</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">back ligation pOmpC+TATA_INSA (D. SP) + TATB_INSB (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,655</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font color="#ff0000" size="1">613</font><font size="1">, 814</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATB_INSB (D. ES)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (E. S.) as front insert (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.27</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.03</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.21</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (P.E.)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">plasmid extraction #1, #3, #4 (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.41, 0.25, 0.41</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_9_27#CinR.2BHSL.2BD-term_check">Sep 27, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (D. E)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">first digestion (E.) as front vector (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.19</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">CinR+HSL+D-term (D. EX)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">second digestion (X.) as front vector (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">4,804</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.19</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.02</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.13</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4">Oct 04, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATB_INSB + CinR+HSL+D-term</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">front ligation TATB_INSB (D. ES) + CinR+HSL+D-term (D. EX) (</font><font color="#ff0000" size="1">wrong!!!</font><font size="1">)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 05, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="center"><font size="1">pOmpC+TATA_INSA+TATB_INSB (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S.P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">2,637</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="center"><font size="1">CinR+HSL+D-term (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X.P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">1,647</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="center"><font size="1"><strong>system 1</strong></font></td><br />
<td align="left"><font size="1">ligation pOmpC+TATA_INSA+TATB_INSB + CinR+HSL+D-term</font></td><br />
<td align="right"><font size="1">4,292</font></td><br />
<td align="right"><font color="#ff0000" size="1">814</font><font size="1">, 2,451</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (P.E. #1)</font></td><br />
<td align="left"><font size="1">plasmid extraction #1</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.23</font></td><br />
<td align="right"><font size="1">1.70</font></td><br />
<td align="right"><font size="1">1.49</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (P.E. #2)</font></td><br />
<td align="left"><font size="1">plasmid extraction #2</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">0.29</font></td><br />
<td align="right"><font size="1">1.58</font></td><br />
<td align="right"><font size="1">1.26</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">pCinRHSL (D. SP)</font></td><br />
<td align="left"><font size="1">digestion (S. P.) as back vector, #1 selected</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.17</font></td><br />
<td align="right"><font size="1">0.03</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">0.18</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_4">Oct 04, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td align="left"><font size="1">OmpRBS (D. XP)</font></td><br />
<td align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td align="right"><font size="1">0.24</font></td><br />
<td align="right"><font size="1">0.01</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1">n/a</font></td><br />
<td align="right"><font size="1"><a href="https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_3">Oct 03, 07</a></font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">ligation pCinRHSL (D. SP) + OmpRBS (D. XP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 05, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as back vector (a bit smear)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">2,443</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.14 (too few)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS (plasmid)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">plasmid extraction of pCinRHSL+OmpRBS (clone 7 and clone 10 on the plate) after liquid culture</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.020; 0.019</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">1.95; 1.83</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2.18; 2.31</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as back vector (smear)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;2,443</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">pCinRHSL+OmpRBS (D. SP)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">digestion (S. P.) pCinRHSL+OmpRBS as <strong>back vector</strong> using plasmid from clone 7</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">2,443</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.05</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">n/a</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">0.22</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">TATD_IDE (D. XP)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="left"><font size="1">digestion (X. P.) as back insert</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">3,190</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">0.10 (too few)</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,204,153)" align="right"><font size="1">Oct 9, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">TATD + IDE (PCR)</font></td><br />
<td style="background-color: rgb(255,255,153)" align="left"><font size="1">re-PCR for TATD + IDE</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">3,203</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(255,255,153)" align="right"><font size="1">Oct 10, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">TATD_IDE (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;3,190</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">Oct 11, 07</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pCinRHSL+OmpRBS + TATD_IDE</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">ligation pCinRHSL+OmpRBS + TATD_IDE</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">pCinRHSL+OmpRBS+TATD_IDE (D. SP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (S. P.) as <strong>back vector</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">D-term (D. XP)</font></td><br />
<td style="background-color: rgb(204,255,255)" align="left"><font size="1">digestion (X. P.) as <strong>back insert</strong></font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right">&nbsp;</td><br />
<td style="background-color: rgb(204,255,255)"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
<td style="background-color: rgb(204,255,255)" align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
<tr align="center"><br />
<td align="center"><font size="1"><strong>system 2</strong></font></td><br />
<td align="left"><font size="1">ligation pCinRHSL+OmpRBS+TATD_IDE + D-term</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right">&nbsp;</td><br />
<td><font size="1">&nbsp;</font></td><br />
<td><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
<td align="right"><font size="1">&nbsp;</font></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<br />
<h3>Notes</h3><br />
<ul><br />
<li>A260/A280 should be around 1.70</li><br />
<li>A260/A230 should be less than 3.00</li><br />
<li>color key<br />
<ul><br />
<li>problemistic (read)</li><br />
<li>finished (yellow)</li><br />
<li>await (blue)</li><br />
</ul><br />
</li><br />
<li>digestion and buffer<br />
<ul><br />
<li>SepI and PstI (<strong>SP</strong>) digestion using buffer <strong>2</strong>, creates back <strong>vector</strong></li><br />
<li>XbaI and PstI (<strong>XP</strong>) digestion using buffer <strong>3</strong>, creates back <strong>insert</strong></li><br />
</ul><br />
</li><br />
<li>Assembly process<br />
<ul><br />
<li><strong>STEP 1</strong>. prepare <strong>insert and vector</strong> by <strong>digestion</strong></li><br />
<li><strong>STEP 2</strong>. check digestion by GEL separation<br />
<ul><br />
<li>if band is clear at correct position (length), cut the gel and purify/extract it</li><br />
<li>else go back to STEP 1.</li><br />
</ul><br />
</li><br />
<li><strong>STEP 3</strong>. <strong>ligate </strong>insert with vector, <strong>transform </strong>it into competent cell, and extract plasmid from liquid culture</li><br />
<li><strong>STEP 4</strong>. check ligation by <strong>VF2, VR PCR</strong> with GEL separation<br />
<ul><br />
<li>if band is clear at correct position (length), cut the gel and purify/extract it (ligation is done)</li><br />
<li>else go back to STEP 3.</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Protocols2
NYMU Taipei/Protocols2
2007-10-19T16:47:28Z
<p>Yfchang: </p>
<hr />
<div><h3>Wet-Lab Protocols</h3><br />
<ul><br />
<li><font size="2">[http://www.protocol-online.org/ Protocol online]</font></li><br />
<li><font size="2">PCR (7/20) </font><br />
<ul><br />
<li><font size="2">RT-PCR </font></li><br />
<li><font size="2">Q-PCR</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Quantitation of&nbsp; Nucleic acid concentration</font><br />
<ul><br />
<li><font size="2">[[http://en.wikipedia.org/wiki/Quantification_of_nucleic_acids Wikipedia]]</font></li><br />
<li><font size="2">[[http://www.piercenet.com/Objects/View.cfm?type=Page&amp;ID=0A0A7BB1-7582-4611-A116-6F583EC01666 Pierce]]</font></li><br />
<li><font size="2">[[http://mcbl.iisc.ernet.in/Welcome%20to%20MCBL/Faculty/Parag/microarray%20workshop%20details/quantitation.html India]]</font></li><br />
<li><font size="2">[[http://www.kreatech.com/pages/Focus-Areas/*-Hybridization-___-Labeling-protocols/Small-RNA-Labeling-protocol/ Kreatech Biotechnology]]&nbsp;</font></li><br />
</ul><br />
</li><br />
<li><font face="Arial" size="2"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial [[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp Setting Up a Restriction Endonuclease Reaction]]</span></font><font size="2"> </font></li><br />
<li><font size="2">DNA Analysis through Gel Electrophoresis</font></li><br />
<li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font><br />
<ul><br />
<li><font size="2">[[http://www.neb.com/nebecomm/products/productM2200.asp Quick Ligation Kit&nbsp;(NEB)]]</font></li><br />
<li><font size="2">[[http://www.ubi.ca/cart/index.php/cPath/27_163_165 Fast Ligation Kit (UBI)]]</font></li><br />
<li><font size="2">[[http://www.epibio.com/item.asp?ID=296 Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)]]</font></li><br />
<li><font size="2">[[http://www.fermentas.com/catalog/kits/kitrapidligation.htm Rapid Ligation Kit (Fermentas)]]</font></li><br />
<li><font size="2">All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.&nbsp;<br /><br />
Use of crowding agents like PEG allow 5-minute sticky end ligations.</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Vector construction </font><br />
<ul><br />
<li><font size="2">Vector sequences </font><br />
<ul><br />
<li>[[http://seq.yeastgenome.org/vectordb/ <font size="2">VectorDB - Molecular Biology Vector Sequence Database</font>]]<font size="2"> </font></li><br />
<li>[[http://www.shigen.nig.ac.jp/cvector/cvector.html <font size="2">The cloning vector collection</font>]]<font size="2"> </font></li><br />
<li>[[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/dna_sequences_maps.asp <font size="2">New England Biolabs (NEB) DNA Sequences and Maps</font>]]<font size="2"> </font></li><br />
<li>[[http://www.promega.com/vectors/cloning_vectors.htm <font size="2">Promega Cloning Vectors</font>]]<font size="2"> </font></li><br />
<li>[[http://www.emdbiosciences.com/Products/BrowseProductsByCategory.asp?catid=1628 <font size="2">Novagen Vector Maps</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li>[[http://wishart.biology.ualberta.ca/PlasMapper/ <font size="2">PlasMapper Version 2.0 - automatically generates and annotates plasmid maps</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><font size="2">[[Transformation]] </font></li><br />
<li><font size="2">[[Production of heterologous proteins in Escherichia coli]] </font></li><br />
<li>[[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks <font size="2">Antibiotics</font>]]<font size="2"> </font></li><br />
<li>[[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication <font face="Arial" size="2">Part fabrication</font>]]<font size="2"> (</font>[[http://openwetware.org/wiki/Enzyme_selection_for_BioBricks_digest <font size="2">Enzyme selection for BioBricks digest</font>]]<font size="2">!!) </font></li><br />
<li>[[http://openwetware.org/wiki/BioBricks_construction_tutorial <font size="2">BioBricks construction tutorial</font>]]<font size="2"> </font></li><br />
<li>[[http://openwetware.org/wiki/Silver:_BB_Strategy <font size="2">Fusion protein</font>]]<font size="2"> </font></li><br />
<li><font size="2">Glucose assay </font><br />
<ul><br />
<li><font color="#000000" size="2">[[http://en.wikipedia.org/wiki/Blood_sugar Blood sugar (from Wikipedia)]]</font><font size="2"> </font></li><br />
<li>[[http://www.genengnews.com/articles/chtitem.aspx?tid=1624&amp;chid=3 <font size="2">Glucose Measurement for Cell Culture</font>]]<font size="2"> </font></li><br />
<li>[[http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Metabolomics/Product_Highlights/Enzymatic_Kits.html <font size="2">Glucose Assay Kits from SigmaAldrich</font>]]<font size="2"> </font></li><br />
<li>[[http://www.bioassaysys.com/Order.html <font size="2">Glucose Assay Kit from QuantiChrom</font>]]<font size="2"> </font></li><br />
<li>[[http://www.rpbiotech.com/Biochem_diag_kits/Glucose_Diagnostic_Kit.htm <font size="2">Biochemical glucose kit from rpbiotech</font>]]<font size="2"> </font></li><br />
<li>[[http://www.anilinepharma.com/products.php?catg=ds&amp;id=1 <font size="2">Glucose Meter from AnilinePharma</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><font size="2">Mammalian Gene Collection (MGC) Full-length cDNA clones </font><br />
<ul><br />
<li>[[http://mgc.nci.nih.gov/ <font size="2">at National Cancer Institute of NIH</font>]]<font size="2"> </font></li><br />
<li>[[http://genome.ym.edu.tw/libres/MGC_clones/index.htm <font size="2">at Genome Research Center of NYMU</font>]]<font size="2"> </font></li><br />
<li>[[http://www.ncbi.nlm.nih.gov/FLC/getmgc.cgi <font size="2">NCBI MGC retrieval</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
</ul><br />
<h3><hr /><br />
</h3><br />
<h3>Dry-Lab Protocols</h3><br />
<ul><br />
<li>[[http://serialbasics.free.fr/Serial_Cloner.html serial cloner]]</li><br />
<li>Protein Fusion Design<br />
<ul><br />
<li>rcsC-envZ cimaera design</li><br />
</ul><br />
<ul><br />
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Literature_reiew Literature review]</li><br />
</ul><br />
<ul><br />
<li>[[Computational analysis]]</li><br />
</ul><br />
</li><br />
<li>Twin-Arginine Translocation Pathway Signal Peptide<br />
<ul><br />
<li>Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395<br />
<ul><br />
<li>[[Properties of TAT Signal Peptides]]</li><br />
<li>[[http://www.jic.bbsrc.ac.uk/staff/tracy-palmer/signals.htm A link to a list of Tat signal sequences provided by Tracy Palmer]]</li><br />
<li>[[29 putative signal peptides]]&nbsp;</li><br />
<li>[[Selected TAT signal peptides]]</li><br />
<li>[[Signal peptides and insulin A, B chain]]</li><br />
<li>[[Signal peptides and IDE coding sequence]]</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>General references</h3><br />
<ul><br />
<li>[[http://www.neb.com/nebecomm/tech_reference/default.asp New England Biolab (NEB) technical references]]</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Protocols2
NYMU Taipei/Protocols2
2007-10-19T16:44:28Z
<p>Yfchang: </p>
<hr />
<div><h3>Wet-Lab Protocols</h3><br />
<ul><br />
<li><font size="2">[http://www.protocol-online.org/ Protocol online]</font></li><br />
<li><font size="2">PCR (7/20) </font><br />
<ul><br />
<li><font size="2">RT-PCR </font></li><br />
<li><font size="2">Q-PCR</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Quantitation of&nbsp; Nucleic acid concentration</font><br />
<ul><br />
<li><font size="2">[[http://en.wikipedia.org/wiki/Quantification_of_nucleic_acids">Wikipedia]]</font></li><br />
<li><font size="2">[[http://www.piercenet.com/Objects/View.cfm?type=Page&amp;ID=0A0A7BB1-7582-4611-A116-6F583EC01666">Pierce]]</font></li><br />
<li><font size="2">[[http://mcbl.iisc.ernet.in/Welcome%20to%20MCBL/Faculty/Parag/microarray%20workshop%20details/quantitation.html">India]]</font></li><br />
<li><font size="2">[[http://www.kreatech.com/pages/Focus-Areas/*-Hybridization-___-Labeling-protocols/Small-RNA-Labeling-protocol/">Kreatech Biotechnology]]&nbsp;</font></li><br />
</ul><br />
</li><br />
<li><font face="Arial" size="2"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial">[[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp">Setting Up a Restriction Endonuclease Reaction]]</span></font><font size="2"> </font></li><br />
<li><font size="2">DNA Analysis through Gel Electrophoresis</font></li><br />
<li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font><br />
<ul><br />
<li><font size="2">[[http://www.neb.com/nebecomm/products/productM2200.asp">Quick Ligation Kit&nbsp;(NEB)]]</font></li><br />
<li><font size="2">[[http://www.ubi.ca/cart/index.php/cPath/27_163_165">Fast Ligation Kit (UBI)]]</font></li><br />
<li><font size="2">[[http://www.epibio.com/item.asp?ID=296">Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)]]</font></li><br />
<li><font size="2">[[http://www.fermentas.com/catalog/kits/kitrapidligation.htm">Rapid Ligation Kit (Fermentas)]]</font></li><br />
<li><font size="2">All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.&nbsp;<br /><br />
Use of crowding agents like PEG allow 5-minute sticky end ligations.</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Vector construction </font><br />
<ul><br />
<li><font size="2">Vector sequences </font><br />
<ul><br />
<li>[[http://seq.yeastgenome.org/vectordb/"><font size="2">VectorDB - Molecular Biology Vector Sequence Database</font>]]<font size="2"> </font></li><br />
<li>[[http://www.shigen.nig.ac.jp/cvector/cvector.html"><font size="2">The cloning vector collection</font>]]<font size="2"> </font></li><br />
<li>[[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/dna_sequences_maps.asp"><font size="2">New England Biolabs (NEB) DNA Sequences and Maps</font>]]<font size="2"> </font></li><br />
<li>[[http://www.promega.com/vectors/cloning_vectors.htm"><font size="2">Promega Cloning Vectors</font>]]<font size="2"> </font></li><br />
<li>[[http://www.emdbiosciences.com/Products/BrowseProductsByCategory.asp?catid=1628"><font size="2">Novagen Vector Maps</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li>[[http://wishart.biology.ualberta.ca/PlasMapper/"><font size="2">PlasMapper Version 2.0 - automatically generates and annotates plasmid maps</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><font size="2">[[Transformation]] </font></li><br />
<li><font size="2">[[Production of heterologous proteins in Escherichia coli]] </font></li><br />
<li>[[http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks"><font size="2">Antibiotics</font>]]<font size="2"> </font></li><br />
<li>[[http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication"><font face="Arial" size="2">Part fabrication</font>]]<font size="2"> (</font>[[http://openwetware.org/wiki/Enzyme_selection_for_BioBricks_digest"><font size="2">Enzyme selection for BioBricks digest</font>]]<font size="2">!!) </font></li><br />
<li>[[http://openwetware.org/wiki/BioBricks_construction_tutorial"><font size="2">BioBricks construction tutorial</font>]]<font size="2"> </font></li><br />
<li>[[http://openwetware.org/wiki/Silver:_BB_Strategy"><font size="2">Fusion protein</font>]]<font size="2"> </font></li><br />
<li><font size="2">Glucose assay </font><br />
<ul><br />
<li><font color="#000000" size="2">[[http://en.wikipedia.org/wiki/Blood_sugar">Blood sugar (from Wikipedia)]]</font><font size="2"> </font></li><br />
<li>[[http://www.genengnews.com/articles/chtitem.aspx?tid=1624&amp;chid=3"><font size="2">Glucose Measurement for Cell Culture</font>]]<font size="2"> </font></li><br />
<li>[[http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Metabolomics/Product_Highlights/Enzymatic_Kits.html"><font size="2">Glucose Assay Kits from SigmaAldrich</font>]]<font size="2"> </font></li><br />
<li>[[http://www.bioassaysys.com/Order.html"><font size="2">Glucose Assay Kit from QuantiChrom</font>]]<font size="2"> </font></li><br />
<li>[[http://www.rpbiotech.com/Biochem_diag_kits/Glucose_Diagnostic_Kit.htm"><font size="2">Biochemical glucose kit from rpbiotech</font>]]<font size="2"> </font></li><br />
<li>[[http://www.anilinepharma.com/products.php?catg=ds&amp;id=1"><font size="2">Glucose Meter from AnilinePharma</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><font size="2">Mammalian Gene Collection (MGC) Full-length cDNA clones </font><br />
<ul><br />
<li>[[http://mgc.nci.nih.gov/"><font size="2">at National Cancer Institute of NIH</font>]]<font size="2"> </font></li><br />
<li>[[http://genome.ym.edu.tw/libres/MGC_clones/index.htm"><font size="2">at Genome Research Center of NYMU</font>]]<font size="2"> </font></li><br />
<li>[[http://www.ncbi.nlm.nih.gov/FLC/getmgc.cgi"><font size="2">NCBI MGC retrieval</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
</ul><br />
<h3><hr /><br />
</h3><br />
<h3>Dry-Lab Protocols</h3><br />
<ul><br />
<li>[[http://serialbasics.free.fr/Serial_Cloner.html">serial cloner]]</li><br />
<li>Protein Fusion Design<br />
<ul><br />
<li>rcsC-envZ cimaera design</li><br />
</ul><br />
<ul><br />
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Literature_reiew Literature review]</li><br />
</ul><br />
<ul><br />
<li>[[Computational analysis]]</li><br />
</ul><br />
</li><br />
<li>Twin-Arginine Translocation Pathway Signal Peptide<br />
<ul><br />
<li>Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395<br />
<ul><br />
<li>[[Properties of TAT Signal Peptides]]</li><br />
<li>[[http://www.jic.bbsrc.ac.uk/staff/tracy-palmer/signals.htm">A link to a list of Tat signal sequences provided by Tracy Palmer]]</li><br />
<li>[[29 putative signal peptides]]&nbsp;</li><br />
<li>[[Selected TAT signal peptides]]</li><br />
<li>[[Signal peptides and insulin A, B chain]]</li><br />
<li>[[Signal peptides and IDE coding sequence]]</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>General references</h3><br />
<ul><br />
<li>[[http://www.neb.com/nebecomm/tech_reference/default.asp">New England Biolab (NEB) technical references]]</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Protocols2
NYMU Taipei/Protocols2
2007-10-19T16:42:42Z
<p>Yfchang: </p>
<hr />
<div><h3>Wet-Lab Protocols</h3><br />
<ul><br />
<li><font size="2">[http://www.protocol-online.org/ Protocol online]</font></li><br />
<li><font size="2">PCR (7/20) </font><br />
<ul><br />
<li><font size="2">RT-PCR </font></li><br />
<li><font size="2">Q-PCR</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Quantitation of&nbsp; Nucleic acid concentration</font><br />
<ul><br />
<li><font size="2"><a href="http://en.wikipedia.org/wiki/Quantification_of_nucleic_acids">Wikipedia]]</font></li><br />
<li><font size="2"><a href="http://www.piercenet.com/Objects/View.cfm?type=Page&amp;ID=0A0A7BB1-7582-4611-A116-6F583EC01666">Pierce]]</font></li><br />
<li><font size="2"><a href="http://mcbl.iisc.ernet.in/Welcome%20to%20MCBL/Faculty/Parag/microarray%20workshop%20details/quantitation.html">India]]</font></li><br />
<li><font size="2"><a href="http://www.kreatech.com/pages/Focus-Areas/*-Hybridization-___-Labeling-protocols/Small-RNA-Labeling-protocol/">Kreatech Biotechnology]]&nbsp;</font></li><br />
</ul><br />
</li><br />
<li><font face="Arial" size="2"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial"><a href="http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp">Setting Up a Restriction Endonuclease Reaction]]</span></font><font size="2"> </font></li><br />
<li><font size="2">DNA Analysis through Gel Electrophoresis</font></li><br />
<li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font><br />
<ul><br />
<li><font size="2"><a href="http://www.neb.com/nebecomm/products/productM2200.asp">Quick Ligation Kit&nbsp;(NEB)]]</font></li><br />
<li><font size="2"><a href="http://www.ubi.ca/cart/index.php/cPath/27_163_165">Fast Ligation Kit (UBI)]]</font></li><br />
<li><font size="2"><a href="http://www.epibio.com/item.asp?ID=296">Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)]]</font></li><br />
<li><font size="2"><a href="http://www.fermentas.com/catalog/kits/kitrapidligation.htm">Rapid Ligation Kit (Fermentas)]]</font></li><br />
<li><font size="2">All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.&nbsp;<br /><br />
Use of crowding agents like PEG allow 5-minute sticky end ligations.</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Vector construction </font><br />
<ul><br />
<li><font size="2">Vector sequences </font><br />
<ul><br />
<li><a href="http://seq.yeastgenome.org/vectordb/"><font size="2">VectorDB - Molecular Biology Vector Sequence Database</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.shigen.nig.ac.jp/cvector/cvector.html"><font size="2">The cloning vector collection</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/dna_sequences_maps.asp"><font size="2">New England Biolabs (NEB) DNA Sequences and Maps</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.promega.com/vectors/cloning_vectors.htm"><font size="2">Promega Cloning Vectors</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.emdbiosciences.com/Products/BrowseProductsByCategory.asp?catid=1628"><font size="2">Novagen Vector Maps</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><a href="http://wishart.biology.ualberta.ca/PlasMapper/"><font size="2">PlasMapper Version 2.0 - automatically generates and annotates plasmid maps</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><font size="2">[[Transformation]] </font></li><br />
<li><font size="2">[[Production of heterologous proteins in Escherichia coli]] </font></li><br />
<li><a href="http://openwetware.org/wiki/Endy:Preparing_Antibiotic_Stocks"><font size="2">Antibiotics</font>]]<font size="2"> </font></li><br />
<li><a href="http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication"><font face="Arial" size="2">Part fabrication</font>]]<font size="2"> (</font><a href="http://openwetware.org/wiki/Enzyme_selection_for_BioBricks_digest"><font size="2">Enzyme selection for BioBricks digest</font>]]<font size="2">!!) </font></li><br />
<li><a href="http://openwetware.org/wiki/BioBricks_construction_tutorial"><font size="2">BioBricks construction tutorial</font>]]<font size="2"> </font></li><br />
<li><a href="http://openwetware.org/wiki/Silver:_BB_Strategy"><font size="2">Fusion protein</font>]]<font size="2"> </font></li><br />
<li><font size="2">Glucose assay </font><br />
<ul><br />
<li><font color="#000000" size="2"><a href="http://en.wikipedia.org/wiki/Blood_sugar">Blood sugar (from Wikipedia)]]</font><font size="2"> </font></li><br />
<li><a href="http://www.genengnews.com/articles/chtitem.aspx?tid=1624&amp;chid=3"><font size="2">Glucose Measurement for Cell Culture</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Metabolomics/Product_Highlights/Enzymatic_Kits.html"><font size="2">Glucose Assay Kits from SigmaAldrich</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.bioassaysys.com/Order.html"><font size="2">Glucose Assay Kit from QuantiChrom</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.rpbiotech.com/Biochem_diag_kits/Glucose_Diagnostic_Kit.htm"><font size="2">Biochemical glucose kit from rpbiotech</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.anilinepharma.com/products.php?catg=ds&amp;id=1"><font size="2">Glucose Meter from AnilinePharma</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
<li><font size="2">Mammalian Gene Collection (MGC) Full-length cDNA clones </font><br />
<ul><br />
<li><a href="http://mgc.nci.nih.gov/"><font size="2">at National Cancer Institute of NIH</font>]]<font size="2"> </font></li><br />
<li><a href="http://genome.ym.edu.tw/libres/MGC_clones/index.htm"><font size="2">at Genome Research Center of NYMU</font>]]<font size="2"> </font></li><br />
<li><a href="http://www.ncbi.nlm.nih.gov/FLC/getmgc.cgi"><font size="2">NCBI MGC retrieval</font>]]<font size="2"> </font></li><br />
</ul><br />
</li><br />
</ul><br />
<h3><hr /><br />
</h3><br />
<h3>Dry-Lab Protocols</h3><br />
<ul><br />
<li><a href="http://serialbasics.free.fr/Serial_Cloner.html">serial cloner]]</li><br />
<li>Protein Fusion Design<br />
<ul><br />
<li>rcsC-envZ cimaera design</li><br />
</ul><br />
<ul><br />
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Literature_reiew Literature review]</li><br />
</ul><br />
<ul><br />
<li>[[Computational analysis]]</li><br />
</ul><br />
</li><br />
<li>Twin-Arginine Translocation Pathway Signal Peptide<br />
<ul><br />
<li>Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395<br />
<ul><br />
<li>[[Properties of TAT Signal Peptides]]</li><br />
<li><a href="http://www.jic.bbsrc.ac.uk/staff/tracy-palmer/signals.htm">A link to a list of Tat signal sequences provided by Tracy Palmer]]</li><br />
<li>[[29 putative signal peptides]]&nbsp;</li><br />
<li>[[Selected TAT signal peptides]]</li><br />
<li>[[Signal peptides and insulin A, B chain]]</li><br />
<li>[[Signal peptides and IDE coding sequence]]</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>General references</h3><br />
<ul><br />
<li><a href="http://www.neb.com/nebecomm/tech_reference/default.asp">New England Biolab (NEB) technical references]]</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Protocols2
NYMU Taipei/Protocols2
2007-10-19T16:37:07Z
<p>Yfchang: </p>
<hr />
<div><h3>Wet-Lab Protocols</h3><br />
<ul><br />
<li><font size="2">[http://www.protocol-online.org/ Protocol online]</font></li><br />
<li><font size="2">PCR (7/20) </font><br />
<ul><br />
<li><font size="2">RT-PCR </font></li><br />
<li><font size="2">Q-PCR</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Quantitation of&nbsp; Nucleic acid concentration</font><br />
<ul><br />
<li><font size="2">[[Wikipedia]]</font></li><br />
<li><font size="2">[[Pierce]]</font></li><br />
<li><font size="2">[[India]]</font></li><br />
<li><font size="2">[[Kreatech Biotechnology]]&nbsp;</font></li><br />
</ul><br />
</li><br />
<li><font face="Arial" size="2"><span lang="EN-US" style="font-size: 9.5pt; font-family: Arial">[[ </font></li><br />
<li><font size="2">DNA Analysis through Gel Electrophoresis</font></li><br />
<li><font size="2">Ligate blunt or cohesive ends in 5 minutes at room temperature</font><br />
<ul><br />
<li><font size="2">[[Quick Ligation Kit&nbsp;(NEB)]]</font></li><br />
<li><font size="2">[[Fast Ligation Kit (UBI)]]</font></li><br />
<li><font size="2">[[Fast-Link DNA Ligation Kit (EPICENTRE Biotechnologies)]]</font></li><br />
<li><font size="2">[[Rapid Ligation Kit (Fermentas)]]</font></li><br />
<li><font size="2">All of these are just normal ligase buffers with PEG8000 at a 10-12% concentration. The NEB catalogue gives the original reference.&nbsp;<br /><br />
Use of crowding agents like PEG allow 5-minute sticky end ligations.</font></li><br />
</ul><br />
</li><br />
<li><font size="2">Vector construction </font><br />
<ul><br />
<li><font size="2">Vector sequences </font><br />
<ul><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
</ul><br />
</li><br />
<li>[[ </font></li><br />
</ul><br />
</li><br />
<li><font size="2">[[Transformation]] </font></li><br />
<li><font size="2">[[Production of heterologous proteins in Escherichia coli]] </font></li><br />
<li>[[ </font></li><br />
<li>[[!!) </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li><font size="2">Glucose assay </font><br />
<ul><br />
<li><font color="#000000" size="2">[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
</ul><br />
</li><br />
<li><font size="2">Mammalian Gene Collection (MGC) Full-length cDNA clones </font><br />
<ul><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
<li>[[ </font></li><br />
</ul><br />
</li><br />
</ul><br />
<h3><hr /><br />
</h3><br />
<h3>Dry-Lab Protocols</h3><br />
<ul><br />
<li>[[serial cloner]]</li><br />
<li>Protein Fusion Design<br />
<ul><br />
<li>rcsC-envZ cimaera design</li><br />
</ul><br />
<ul><br />
<li>[https://2007.igem.org/NYMU_Taipei/Protocols2/Literature_reiew Literature review]</li><br />
</ul><br />
<ul><br />
<li>[[Computational analysis]]</li><br />
</ul><br />
</li><br />
<li>Twin-Arginine Translocation Pathway Signal Peptide<br />
<ul><br />
<li>Lee et al., 2006. The Bacterial Twin-Arginine Translocation Pathway. Annu. Rev. Microbiol., 60, p373-395<br />
<ul><br />
<li>[[Properties of TAT Signal Peptides]]</li><br />
<li>[[A link to a list of Tat signal sequences provided by Tracy Palmer]]</li><br />
<li>[[29 putative signal peptides]]&nbsp;</li><br />
<li>[[Selected TAT signal peptides]]</li><br />
<li>[[Signal peptides and insulin A, B chain]]</li><br />
<li>[[Signal peptides and IDE coding sequence]]</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>General references</h3><br />
<ul><br />
<li>[[New England Biolab (NEB) technical references]]</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Help_me!
NYMU Taipei/Help me!
2007-10-19T16:35:40Z
<p>Yfchang: </p>
<hr />
<div><h3>Q1: Infrared Emitting Protein</h3><br />
<ul><br />
<li><font size="2">I've found parts that cantranslate green, yellow, and red fluorescence protein, but I can't find that can emit <strong>infrared rays</strong>. Can&nbsp;anyone help me find it? (posted by De-Ming Lin) </font><br />
<ul><br />
<li><font size="2">I am not sure there is a one can emit infrared light.</font></li><br />
</ul><br />
</li><br />
<li><font size="2">What is your original idea for having the special part?</font><br />
<ul><br />
<li>If we can combine glucose promoter and structural gene which can <font size="2">translate</font> <font size="2">protein that can emit </font><font size="2">infrared </font><font size="2">ray, and put it on the finger tip, we can measure blood sugar by an non-invasive way.</font></li><br />
<li><font size="2">I think it is the question about how to measure the promoter activity under glucose stimulus in our system (8/30).</font></li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>Q2: Documentation of Projects</h3><br />
<ul><br />
<li><font size="2">For documentation, please all create a page for each of the five candidate projects on &quot;My hot teams&quot; and describe your design there. Uploading all the files of powerpoint or reference literature is very welcome! Thank you all very much for efforts of documentation ^^ </font><font size="2">Can we just document the two selected projects?</font><br />
<ul><br />
<li>Reply: I just want to make the &quot;brainstorming&quot; part of our documentation as lush as we can ^^&quot; I know everybody is busy, so maybe some description words with some files already done (powerpoint and literature showed on 7/13) are plenty enough ^^</li><br />
</ul><br />
</li><br />
<li>Should we have a good framework about how to record our experiments and ideas? (8/30)</li><br />
</ul><br />
<hr /><br />
<h3>Q3: Problems about [[Materials]] and Designs</h3><br />
<ul><br />
<li><font size="2">由於沒有材料,我們無法動手進行實驗; P200、P400 Promoter 序列.......也無法確定,要火速解決! (posted by 迪璿, partially solved)</font><br />
<ul><br />
<li><font size="2">design has been changed. There is no P200 or P400 promoters in our current planning.</font></li><br />
</ul><br />
</li><br />
<li>Insulin gene的來源 (solved)<br />
<ul><br />
<li>[http://www.dnai.org/text/mediashowcase/index2.html?id=329 A plasmid vector for expressing the human insulin gene in nonendocrine cells, 1994]</li><br />
<li>A method for cloning the insulin gene from total human DNA is developed on the basis of the polymerase chain reaction. Cloning of the insulin gene from the <strong>total DNA of human lymphocytes</strong> is performed to obtain a <strong>pNP-90</strong> vector for subsequent expression of the insulin gene in nonendocrine cells for the purposes of gene therapy of diabetic angiopathies</li><br />
<li>[http://www.dnai.org/text/mediashowcase/index2.html?id=329 Cloning the human insulin gene, Walter Gilbert]</li><br />
<li>[http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro10.swf::Steps%20in%20Cloning%20a%20Gene steps in gene cloning]</li><br />
<li>we had the clone of human insulin from genomics center of NYMU, please see ...</li><br />
</ul><br />
</li><br />
<li>glucose sensing promoter的來源 (solved)<br />
<ul><br />
<li>[https://2007.igem.org/NYMU_Taipei/Help_me!/Regulated_insulin_serection_Team Meeting_2007/07/30 Regulated insulin serection Team Meeting 2007/07/30] (alternative method)</li><br />
<li>ompR binding site<br />
<ul><br />
<li>[http://partsregistry.org/Part:BBa_R0082 Part:BBa_R0082] (15P, plate 1), pOmpC</li><br />
<li>[http://partsregistry.org/Part:BBa_R0083 Part:BBa_R0083] (17H, plate 1), partial pOmpC</li><br />
<li[http://partsregistry.org/Part:BBa_R0084 Part:BBa_R0084] (11H, plate 1), OmpF</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
<li>How to transport insulin?<br />
<ul><br />
<li>From the research of Japanese scientist, we have known that fusion proteins with an <strong>alpha-hemolysin (HlyA) C-terminal signal sequence</strong> are known to be secreted by the HlyB-HlyD-TolC translocator in Escherichia coli. And in their research, a particular mutant of hlyB and hlyD can achive higher transport efficiency. (The full article is [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&amp;pubmedid=15691914 here], and the source link in our site is [http://igem.ym.edu.tw/private_2007/index.php/Production_of_heterologous_proteins_in_Escherichia_coli here])</li><br />
<li>The <strong>twin arginine (Tat) </strong>secretion pathway allows the translocation of folded proteins across the cytoplasmic membrane of bacteria. Tat-specific signal peptides contain a characteristic amino acid motif <strong>((S/T)<font color="#ff0000">RR</font>XFLK) </strong>including two highly conserved consecutive arginine residues that are thought to be involved in the recognition of the signal peptides by the Tat translocase. (The full story can be found in [http://igem.ym.edu.tw/private_2007/index.php/Disulfide_bond_formation_in_Escherichia_coli here]. Source article is [[here]] and another article is [http://www.jbc.org/cgi/content/full/282/11/8309 here])</li><br />
<li>design of primers for constructing INS_A, INS_B, IDE with TAT signal peptides</li><br />
</ul><br />
</li><br />
<li>How to activate insulin?<br />
<ul><br />
<li>Owing to that insulin requires the di-sufur binding between chain A and B to activate its activity, and <em>E.coli</em> seldomly forms di-sufur bounds in the cytoplasma under highly reduced environment, the solution is to mutant <font size="2"><font color="#0000ff">glutathione oxidoreductase (<em><font color="#ff0000">gor</font></em>)</font> and/or <font color="#0000ff">thioredoxin reductase (<em><font color="#ff0000">trxB</font></em>)</font> genes to decrease the reducing power within the membrane. See also the [http://igem.ym.edu.tw/private_2007/index.php/Image:Disulfide_reducing.jpg di-sulfur reducing pathways]. The current solution is to buy E.coli with mutants on gene gor and trxB (find out the strain we need [http://wolfson.huji.ac.il/expression/Bacterial_Strains.htm#strains-exp here]). The full story can be found [http://igem.ym.edu.tw/private_2007/index.php/Disulfide_bond_formation_in_Escherichia_coli here] (Thanks for the sharing from Dr. Chang)</font></li><br />
<li><font size="2">Besides, we made the </font>insulin to be expressed separately in alpha and beta chains only instead of the full length</li><br />
</ul><br />
</li><br />
<li>How to verify our insulin?<br />
<ul><br />
<li>antibody of insulin</li><br />
</ul><br />
</li><br />
<li>How to inhibit our insulin?<br />
<ul><br />
<li>IDE (insulin degrdation enzyme)</li><br />
</ul><br />
</li><br />
<li>How to prevent <em>E.coli</em> intakes the glucose?</li><br />
</ul><br />
<hr /><br />
<h3>Q4: how to read the plate in iGEM 2007 Kit?</h3><br />
<ul><br />
<li>[[Image:IGEM06DistPlateTop.jpg]]</li><br />
<li>please make sure the side down has two cutting edges (triangle) to ensure the correctness of orientation</li><br />
</ul><br />
<hr width="100%" size="2" /><br />
<h3>Q5: Detail sequence information needed for the parts in our design</h3><br />
<ul><br />
<li>We need your help to find the sequence information of the parts in our design and annotate the sequence in readable format so experimental detail could be determined. Thank you!</li><br />
<li>Parts need detail sequence information and annotation ASAP: RcsC,&nbsp; EnrZ, [http://partsregistry.org/Part:BBa_R0082 Part:BBa_R0082] (15P, plate 1), [http://partsregistry.org/Part:BBa_R0083 Part:BBa_R0083] (17H, plate 1), [http://partsregistry.org/Part:BBa_R0084 Part:BBa_R0084] (11H, plate 1), insulin construct from<font size="2"> [[BCRC]]</font></li><br />
<li>For more information, refer to [https://2007.igem.org/NYMU_Taipei/Help_me/planning planning]. If you are interested in experimental design, please feel free to write down your comment on the discussion page of [https://2007.igem.org/NYMU_Taipei/Help_me/planning planning]. Thank you!</li><br />
</ul><br />
<hr /><br />
<h3>Q6: Requests for chemicals</h3><br />
<ul><br />
<li>Anthrone for [glucose-detection assay] (posted by A ken)</li><br />
<li>NdeI in part tar-EnvZ (got it!)</li><br />
<li>SpeI in standard assembly (got it!)</li><br />
<li>chloramphenicol (10-20ug/ml, methanol as solution, $29 for 5g) (got it!)</li><br />
<li>human insulin<br />
<ul><br />
<li>林宜賢醫師說消防隊附近的川秀藥局 (2827-0702) 有賣</li><br />
<li>川秀藥局 sold (8/30, 2007)<br />
<ul><br />
<li>Humulin N [http://www.lillydiabetes.com/product/humulin_family.jsp?reqNavId=5.3 Lilly]), 中效型 (1 time/day, 混濁狀), 售價 350 NTD</li><br />
<li>Humulin R [http://www.lillydiabetes.com/product/humulin_family.jsp?reqNavId=5.3 Lilly]), 常規型 (2 times/day, 澄清狀), 售價 350 NTD</li><br />
<li>Actrapid ([http://www.novonordisk.com/diabetes/hcp/pharmaceuticals/actrapid/default.asp?bIsPro=true Novo nordisk]), 短效型 (short-acting, 澄清狀), 售價 450 NTD</li><br />
<li>Insulatard ([http://www.novonordisk.com/diabetes/hcp/pharmaceuticals/insulatard/default.asp Novo nordisk]), 中效型 (intermediate-acting, 混濁狀), 售價 400 NTD</li><br />
</ul><br />
</li><br />
</ul><br />
<ul><br />
<li>sigma insulin</li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>Q7: How to detect the glucose concentration in our project?</h3><br />
<hr /><br />
<h3>Q8: How to participate our wet lab works?</h3><br />
<ul><br />
<li>Assembly procedure for two parts<br />
<ul><br />
<li>STEP 1: digest full plasmid into part DNA and vector DNA (2.0 h waiting, 0.5 h operation; total 2.5 h)</li><br />
<li>STEP 2: gel separation for part DNA and vector DNA (1.0 h waiting, 0.5 h operation; total 1.5 h)</li><br />
<li>STEP 3: ligation for 2 part DNAs (3.0 h waiting, 0.5 h operation; total 3.5 h)</li><br />
<li>STEP 4: transformation in plate culture (16.0 h waiting, 0.5 h operation; total 16.5 h)</li><br />
<li>STEP 5: liquid culture to amplify the combined part DNA (16.0 h waiting, 0.0 h operation; total 16.0 h), back to STEP 1 and repeat</li><br />
</ul><br />
</li><br />
</ul><br />
<hr /><br />
<h3>Q9: We need an &quot;available restriction enzymes list and an almost depleted reagents page.</h3><br />
<ul><br />
<li><[http://igem.ym.edu.tw/private_2007/index.php/Available_Restriction_Enzymes Available Restriction Enzymes]</li><br />
<li>[http://igem.ym.edu.tw/private_2007/index.php/Almost_Depleted_Reagents Almost Depleted Reagents]</li><br />
</ul><br />
<hr /><br />
<h3>Q10: How about the hotels for Jamboree 2007?</h3><br />
<ul><br />
<li>[https://2007.igem.org/Jamboree/Hotels https://2007.igem.org/Jamboree/Hotels]</li><br />
<li>There are three Holiday Inn in Boston and each provides different prices!</li><br />
<li>Holiday Inn Express Hotel &amp; Suites Cambridge/Boston<br />
<ul><br />
<li>Price: $149US plus applicable tax</li><br />
<li>Transportation to/from: 15-20 minute walk to campus</li><br />
</ul><br />
</li><br />
</ul><br />
<ul><br />
<li>Holiday Inn: Boston Government Center<br />
<ul><br />
<li>Price: $179US plus applicable tax</li><br />
<li>Transportation to/from: 15-20 minute scenic walk across the Charles river to the Jamboree or 2 minute walk to Charles/MGH T stop ([[MBTA]]) on the Red line (take to Kendall Square to get to Jamboree)</li><br />
</ul><br />
</li><br />
<li>Holiday Inn: Somerville<br />
<ul><br />
<li>Price: $139US plus applicable tax</li><br />
<li>Transportation to/from:</li><br />
</ul><br />
</li><br />
</ul><br />
<hr width="100%" size="2" /><br />
<h3>Q11: About apply the US visa</h3><br />
<ul><br />
<li>[http://www.ait.org.tw/zh/visa/niv/ http://www.ait.org.tw/zh/visa/niv/]</li><br />
<li>Everybody who do not have a valid US visa should apply for the visa and arrange the appointment as soon as possible.</li><br />
<li>Take special-sized photos, pay the fee, finish the online application form, and appoint the interview (Engine: 10/3 10:00)</li><br />
<li>[https://2007.igem.orglogin.cgi https://2007.igem.orglogin.cgi]&nbsp;-&gt; log in -&gt; and apply an invitation letter to bring to the interview</li><br />
</ul><br />
<hr /><br />
<h3>Q12: For boys</h3><br />
<ul><br />
<li><font size="2">[https://nas.immigration.gov.tw/ https://nas.immigration.gov.tw/]</font></li><br />
<li><font size="2">Finish the DS-157 form during applying US visa</font></li><br />
</ul><br />
<p>&nbsp;</p><br />
<hr /><br />
<p>&nbsp;</p><br />
<h3>Q13: Documentation</h3><br />
<p>[https://2007.igem.org/Jamboree/Compete#Online_Judging https://2007.igem.org/Jamboree/Compete#Online_Judging]</p><br />
<ul><br />
<ul><br />
<li><br />
<h2>Project Documentation on the iGEM Wiki</h2><br />
</li><br />
</ul><br />
<li><strong>Description</strong>: The team's project should be documented on the iGEM 2007 wiki site with detail enough to replicate it independently. Additional presentational information about the team - their story, the rationale for the project, failures, successes, future work, etc. - is highly encouraged. Remember that these wiki pages will be the main source of inspiration for future teams, and having good documentation on them and in the part description in the registry increases the likelihood of more teams building on your project and your parts.</li><br />
<li><strong>deadline</strong>: the iGEM wiki freezes 04:59 GMT Oct 27</li><br />
</ul><br />
<ul><br />
<ul><br />
<li><br />
<h2>Part Documentation on the Registry</h2><br />
</li><br />
</ul><br />
<li><strong>Description</strong>: Document your parts well - the success of not just iGEM, but all of synthetic biology, depends on the development of well-characterized, reliable, standardized biological parts that have been designed to be simple to use and understand. The first step is complete documentation.</li><br />
<li><strong>deadline</strong>: the Registry freezes 04:59 GMT Oct 27</li><br />
</ul><br />
<p>&nbsp;</p><br />
<blockquote dir="ltr" style="margin-right: 0px"><br />
<p>Plan to document:</p><br />
</blockquote><br />
<ul><br />
<li>Regular meeting</li><br />
<li>My hot teams</li><br />
<li>Team schedule</li><br />
<li>Help me!</li><br />
<li>Protocols</li><br />
<li>Materials</li><br />
<li>Benchman</li><br />
<li>Our Part Registry&nbsp;</li><br />
<li>Key experiments</li><br />
<li>Construct</li><br />
<li id="n-Almost-Depleted-Reagents">Almost Depleted Reagents</li><br />
<li id="n-You-must-know.21">You must know!</li><br />
<li id="n-Paper">Paper</li><br />
</ul><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Team_schedule
NYMU Taipei/Team schedule
2007-10-19T16:32:53Z
<p>Yfchang: </p>
<hr />
<div><h3>July 2007</h3><br />
<p><br />
<table style="text-align: left" width="50%" border="1"><br />
<br />
<tr><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sun.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Mon.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Tue.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Wed.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Thr.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Fri.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sat.</big></td><br />
</tr><br />
<tr><br />
<td align="center">1</td><br />
<td align="center">2</td><br />
<td align="center">3</td><br />
<td align="center">4</td><br />
<td align="center">5</td><br />
<td align="center">[[2007/07/06|6]]</td><br />
<td align="center">7</td><br />
</tr><br />
<tr><br />
<td align="center">8</td><br />
<td align="center">9</td><br />
<td align="center">10</td><br />
<td align="center">11</td><br />
<td align="center">12</td><br />
<td align="center">[[2007/07/13|13]]</td><br />
<td align="center">14</td><br />
</tr><br />
<tr><br />
<td align="center">15</td><br />
<td align="center">[[2007/07/16|16]]</td><br />
<td align="center">17</td><br />
<td align="center">18</td><br />
<td align="center">19</td><br />
<td align="center">[[2007/07/20|20]]</td><br />
<td align="center">[[2007/07/21|21]]</td><br />
</tr><br />
<tr><br />
<td align="center">22</td><br />
<td align="center">23</td><br />
<td align="center">[[2007/07/24|24]]</td><br />
<td align="center">25</td><br />
<td align="center">26</td><br />
<td align="center">27</td><br />
<td align="center">28</td><br />
</tr><br />
<tr><br />
<td align="center">29</td><br />
<td align="center">30</td><br />
<td align="center">31</td><br />
<td align="center">&nbsp;</td><br />
<td align="center">&nbsp;</td><br />
<td align="center">&nbsp;</td><br />
<td align="center">&nbsp;</td><br />
</tr><br />
<br />
</table><br />
</p><br />
<p>&nbsp;</p><br />
<h3>Aug 2007</h3><br />
<p><br />
<table style="text-align: left" width="50%" border="1"><br />
<br />
<tr><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sun.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Mon.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Tue.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Wed.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Thr.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Fri.</big></td><br />
<td style="font-weight: bold; background-color: rgb(204,255,204); text-align: center"><big>Sat.</big></td><br />
</tr><br />
<tr><br />
<td align="center">&nbsp;</td><br />
<td align="center">&nbsp;</td><br />
<td align="center">&nbsp;</td><br />
<td align="center">1</td><br />
<td align="center">2</td><br />
<td align="center">3</td><br />
<td align="center">4</td><br />
</tr><br />
<tr><br />
<td align="center">5</td><br />
<td align="center">[[2007/08/06|6]]</td><br />
<td align="center">7</td><br />
<td align="center">8</td><br />
<td align="center">9</td><br />
<td align="center">10</td><br />
<td align="center">11</td><br />
</tr><br />
<tr><br />
<td align="center">12</td><br />
<td align="center">13</td><br />
<td align="center">14</td><br />
<td align="center">15</td><br />
<td align="center">16</td><br />
<td align="center">17</td><br />
<td align="center">18</td><br />
</tr><br />
<tr><br />
<td align="center">19</td><br />
<td align="center">20</td><br />
<td align="center">21</td><br />
<td align="center">22</td><br />
<td align="center">23</td><br />
<td align="center">24</td><br />
<td align="center">25</td><br />
</tr><br />
<tr><br />
<td align="center">26</td><br />
<td align="center">27</td><br />
<td align="center">28</td><br />
<td align="center">29</td><br />
<td align="center">30</td><br />
<td align="center">31</td><br />
<td align="center">&nbsp;</td><br />
</tr><br />
<br />
</table><br />
</p><br />
<p>&nbsp;</p><br />
<h3>System 1 assembly</h3><br />
<div align="center">&nbsp;</div><br />
<p><br />
<table border="1"><br />
<br />
<tr><br />
<td align="center"><strong>Run</strong></td><br />
<td align="center"><strong>Date and Time<br /><br />
</strong></td><br />
<td align="center"><strong>Objective</strong></td><br />
<td align="center"><strong>Attendance</strong></td><br />
</tr><br />
<tr><br />
<td style="background-color: rgb(255,255,255)" align="center">1</td><br />
<td style="background-color: rgb(255,255,255)">8 AM Sat, 9/1, 07</td><br />
<td style="background-color: rgb(255,255,255)"><br />
<ul><br />
<li>(RBS + cinR) + (RBS + HSL) = cinR + HSL</li><br />
<li>RBS + cinR ver. 2</li><br />
<li>plate culture for (cinR + HSL) and (RBS + CinR) ver.2</li><br />
</ul><br />
</td><br />
<td style="background-color: rgb(255,255,255)" align="center">茹, 翔</td><br />
</tr><br />
<tr><br />
<td style="background-color: rgb(255,255,255)" align="center">2</td><br />
<td style="background-color: rgb(255,255,255)">8 AM Sun, 9/2, 07</td><br />
<td style="background-color: rgb(255,255,255)"><br />
<ul><br />
<li>Liquid culture for (cinR + HSL) and (RBS + CinR) ver.2</li><br />
<li>[https://2007.igem.org/NYMU_Taipei/Team_schedule/classify_parts_in_box classify parts in box]</li><br />
</ul><br />
</td><br />
<td style="background-color: rgb(255,255,255)" align="center">雄, 威, 茹, 駿</td><br />
</tr><br />
<tr><br />
<td style="background-color: rgb(255,255,255)" align="center">3</td><br />
<td style="background-color: rgb(255,255,255)">8 AM Mon, 9/3, 07</td><br />
<td style="background-color: rgb(255,255,255)"><br />
<ul><br />
<li>plasmid extraction for (cinR + HSL) and (RBS + CinR) ver.2</li><br />
<li>pOmpC + (RBS + INS_A)</li><br />
<li>(RBS + cinR) + (RBS + HSL) + D-term</li><br />
</ul><br />
</td><br />
<td style="background-color: rgb(255,255,255)" align="center">宥, 君, 威</td><br />
</tr><br />
<tr><br />
<td style="background-color: rgb(255,255,255)" align="center">4</td><br />
<td style="background-color: rgb(255,255,255)">8 AM Tue, 9/4, 07</td><br />
<td style="background-color: rgb(255,255,255)"><br />
<ul><br />
<li>plate culture for ...</li><br />
<li>Measure the concentration of all excised parts<br />
<ul><br />
<li>(cinR + HSL)</li><br />
<li>(RBS + CinR) ver.2</li><br />
</ul><br />
</li><br />
<li>BsaBI digestion of cinR</li><br />
</ul><br />
</td><br />
<td style="background-color: rgb(255,255,255)" align="center">宥, 君, 威</td><br />
</tr><br />
<tr><br />
<td align="center">5</td><br />
<td>8 AM Wed, 9/5, 07</td><br />
<td><br />
<ul><br />
<li>(RBS + INS_A), PCR #1</li><br />
<li>(RBS + INS_B), PCR #1</li><br />
<li>pOmpC + (RBS + INS_A), not check yet</li><br />
<li>pOmpC + (RBS + INS_B), not check yet</li><br />
</ul><br />
</td><br />
<td align="center">威, 茹</td><br />
</tr><br />
<tr><br />
<td align="center">6</td><br />
<td>8 AM Thr, 9/6, 07</td><br />
<td><br />
<ul><br />
<li>N/A</li><br />
</ul><br />
</td><br />
<td align="center">威</td><br />
</tr><br />
<tr><br />
<td align="center">7</td><br />
<td>8 AM Fri, 9/7, 07</td><br />
<td><br />
<ul><br />
<li>N/A</li><br />
</ul><br />
</td><br />
<td align="center">儒, 威, 茹</td><br />
</tr><br />
<tr><br />
<td align="center">8</td><br />
<td>8 AM Sat, 9/8, 07</td><br />
<td><br />
<ul><br />
<li>(RBS+INS_A), PCR #2</li><br />
<li>(RBS+INS_B), PCR #2</li><br />
<li>GEL check (5 tubes for each gene)<br />
<ul><br />
<li>INS_A has two tubes A1 (0.08g), A3 (0.15g)</li><br />
<li>INS_B has five tubes B1 (0.12g), B2 (0.12g), B3 (0.16g), B4 (0.11g), B5 (0.17g)</li><br />
</ul><br />
</li><br />
<li>ligation for INS_A and pOmpC</li><br />
<li>plate culture for (pOmpC+INS_A and pOmpC+INS_B), transformation <font color="#ff0000">failed</font><br />
<ul><br />
<li>forget to add vector</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
<td align="center">倫, 茹</td><br />
</tr><br />
<tr><br />
<td align="center">9</td><br />
<td>8 AM Sun, 9/9, 07</td><br />
<td><br />
<ul><br />
<li>plate culture <font color="#ff0000">failed</font> (possible due to absent of vector, checked by text paper record)</li><br />
<li>re-ligate INS_A insert into vector with pOmpC</li><br />
<li>re-check the concentration of insert INS_A by <a href="http://rsb.info.nih.gov/ij/">imageJ</a></li><br />
<li>plate culture again for pOmpC+INS_A<br />
<ul><br />
<li>1uL insert + 1uL vector</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
<td align="center">倫, 翔</td><br />
</tr><br />
<tr><br />
<td align="center">10</td><br />
<td>4 PM Mon, 9/10, 07</td><br />
<td><br />
<ul><br />
<li>plate culture is still <font color="#ff0000">failed</font></li><br />
<li>re-build pOmpC + INS_A in two different insert concentration<br />
<ul><br />
<li>2uL insert + 1uL vector</li><br />
<li>3uL insert + 1uL vector</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
<td align="center">倫, 翔, 銘</td><br />
</tr><br />
<tr><br />
<td align="center">11</td><br />
<td>6 PM Thu, 9/11, 07</td><br />
<td><br />
<ul><br />
<li>re-measure the concentration of insert by biophotometer<br />
<ul><br />
<li>dsDNA use A260 OD to estimate, 1 A260 OD = 50 mg/mL = 0.05 ug/uL</li><br />
<li>OD ranged from 0.1 - 2.0 is located in linear region</li><br />
<li>OD close to zero is not reliable</li><br />
</ul><br />
</li><br />
<li>test for D7 and D8 (9/8 PCR #2) in box #1</li><br />
</ul><br />
</td><br />
<td align="center">銘</td><br />
</tr><br />
<tr><br />
<td align="center">12</td><br />
<td>6 PM Wed, 9/12, 07</td><br />
<td><br />
<ul><br />
<li>check (9/5 PCR) by GEL separation<br />
<ul><br />
<li>concentration of INS_A is still too low</li><br />
</ul><br />
</li><br />
<li>RE-examine the condition of PCR in different annealing temperature<br />
<ul><br />
<li>PCR INS_A in 49.8, 52.3, 55.5, 58.7 and 60.9 degree</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
<td align="center">銘</td><br />
</tr><br />
<tr><br />
<td align="center">13</td><br />
<td>6 PM Thur, 9/13, 07</td><br />
<td><br />
<ul><br />
<li>check (9/12 PCR) by GEL separation<br />
<ul><br />
<li>band seems O.K.</li><br />
<li>best annealing temperature is around 55.5</li><br />
</ul><br />
</li><br />
<li>biophotometer concentration test<br />
<ul><br />
<li>9/5 and 9/12 PCR seem O.K.</li><br />
<li>9/8 PCR seem problemistic due to A260/A280 more than 10</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
<td align="center">銘</td><br />
</tr><br />
<tr><br />
<td align="center">14</td><br />
<td>6 PM Sun, 9/16, 07</td><br />
<td><br />
<ul><br />
<li>digest CinR+HSL (ES) and D-term (EX)and GEL separation check<br />
<ul><br />
<li>tube #1 and #2 of CinR+HSL plasmid extraction is not correct after GEL check</li><br />
<li>first digestion of D-term (EcoRI) is not very sucessful<br />
<ul><br />
<li>thus pett re-digest D-term with double enzyme amount</li><br />
</ul><br />
</li><br />
</ul><br />
</li><br />
</ul><br />
</td><br />
<td align="center">宥, 君, 翔</td><br />
</tr><br />
<tr><br />
<td align="center">PCR</td><br />
<td>Thur, 9/27, 07</td><br />
<td><br />
<ul><br />
<li>PCR of TAT_INS_A, TAT_INS_B, OmpRBS, TAT_IDE</li><br />
</ul><br />
</td><br />
<td align="center">威</td><br />
</tr><br />
</table><br />
</p><br />
<p>&nbsp;</p><br />
[https://2007.igem.org/NYMU_Taipei/Welcome Back]</div>
Yfchang
http://2007.igem.org/wiki/index.php/Test
Test
2007-10-19T13:17:00Z
<p>Yfchang: </p>
<hr />
<div></div>
Yfchang
http://2007.igem.org/wiki/index.php/Test
Test
2007-10-19T13:16:49Z
<p>Yfchang: </p>
<hr />
<div><p><font size="4" color="#ff0000"><strong>Latest News<br /><br />
</strong></font></p><br />
<ul><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial"><a href="http://www.syntheticbiology3.ethz.ch/monday.htm">Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference</a> are now available.<br /><br />
<br /><br />
</font></font></li><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the <a href="http://pub.ucsf.edu/today/cache/feature/200708022.html">UCSF Summer Assignment: Genetically Engineer a New Cell</a>.</font></font>&nbsp;&nbsp;</li><br />
</ul><br />
<ul><br /><br />
<li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /><br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><br />
<font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /><br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /><br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /><br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font><a href="http://www.ym.edu.tw/map.html"><font size="2">check map here!</font></a><font size="2"> We are at the P3 location on the map)</font>&nbsp;</li><br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - <a href="http://www.ym.edu.tw/">National Yang Ming University</a><br /><br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" /></td><br />
</tr><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" /></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster (<a href="http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg">download full size version</a>)<br /><br />
</strong></font></font></p><br />
<p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" /> <img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" />&nbsp;</p></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-19T13:16:41Z
<p>Yfchang: </p>
<hr />
<div>[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]<br />
<br />
[[test]]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-19T13:16:29Z
<p>Yfchang: </p>
<hr />
<div>[https://2007.igem.org/NYMU_Taipei/ProjectDescription Team project]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Regular_meeting Regular meeting]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/My_hot_teams Brainstroming] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Team_schedule Team schedule] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Help_me! Help me!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Protocols2 Protocols] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Materials Materials] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Benchman Benchman] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Our_Part_Registry Our Part Registry] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Key_experiments Key experiments] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Costruct Construct] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Available_Restriction_Enzymes Available Restriction Enzymes]<br />
<br />
[https://2007.igem.org/NYMU_Taipei/Almost_Depleted_Reagents Almost Depleted Reagents] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/You_must_know! You must know!] <br />
<br />
[https://2007.igem.org/NYMU_Taipei/Paper Paper]<br />
<br />
[https://2007.igem.org/Taipei Back]<br />
<br />
[test]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:19:03Z
<p>Yfchang: </p>
<hr />
<div><div id="globalWrapper"><div id="column-content"><div id="content"><br />
<br />
=IGEM 2007 at NYMU, Taiwan=<br />
<br />
<div id="bodyContent"><br />
<br />
===From NYMU_iGEM2007===<br />
<br />
<div id="contentSub">(Redirected from [/iGEM2007/index.php?title=Main_Page&redirect=no Main Page])</div><div id="jump-to-nav">Jump to: [#column-one navigation], [#searchInput search]</div><br />
<br />
<font size="4" color="#ff0000">'''Latest News<br />'''</font><br />
<br />
* <font size="3" color="#000000" face="Verdana"><font face="Arial">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of "Synthetic Biology 3.0" conference] are now available.<br /><br /></font></font><br />
* <font size="3" color="#000000" face="Verdana"><font face="Arial">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font></font><br />
<br />
<br /><br />
* <font size="4" color="#993366" face="Verdana">'''Our NYMU iGEM team meets regularly every week.<br />'''<font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><font size="2">[[Image:cry_smile.gif]]'' no matter if you are a formal member of our team or ''</font><font size="2"><font color="#000000">''not''.<br /></font><br /> The regular meeting for our team is scheduled as follows: <br />'''<font color="#009900">Time & Date:</font>''' 6:00 pm on every <font color="#0000ff">'''Friday'''</font><br />'''<font color="#009900">Place:</font>''' Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html <font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font><br />
<br />
----<br />
<br />
<font size="4" color="#0000ff">'''The Beauty of our School - [http://www.ym.edu.tw/ National Yang Ming University]<br />'''</font><br />
<br />
{|<br />
|<br />
[[Image:P04.jpg]]<br />
|<br />
[[Image:P03.jpg]]<br />
|-<br />
|<br />
[[Image:Y02.jpg]]<br />
|<br />
[[Image:Y09.jpg]]<br />
|}<br />
<br />
----<br />
<br />
<font size="4"> <font color="#0000ff">'''Our iGEM2007 Mascot and Recruitment Poster ([http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg download full size version])<br />'''</font></font><br />
<br />
[[Image:Image_logo.jpg|logo]] [[Image:Image_poster.jpg|poster]]<br />
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</div></div></div><div id="column-one"><div id="p-cactions" class="portlet"><br />
<br />
=====Views=====<br />
<br />
<div class="pBody"><br />
<br />
* [/iGEM2007/index.php/IGEM_2007_at_NYMU%2C_Taiwan Article]<br />
* [/iGEM2007/index.php?title=Talk:IGEM_2007_at_NYMU%2C_Taiwan&action=edit Discussion]<br />
* [/iGEM2007/index.php?title=IGEM_2007_at_NYMU%2C_Taiwan&action=edit Edit]<br />
* [/iGEM2007/index.php?title=IGEM_2007_at_NYMU%2C_Taiwan&action=history History]<br />
* [/iGEM2007/index.php/Special:Movepage/IGEM_2007_at_NYMU%2C_Taiwan Move]<br />
* [/iGEM2007/index.php?title=IGEM_2007_at_NYMU%2C_Taiwan&action=watch Watch]<br />
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</div></div><div id="p-personal" class="portlet"><br />
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=====Personal tools=====<br />
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* [/iGEM2007/index.php/User:Ian Ian]<br />
* [/iGEM2007/index.php/User_talk:Ian My talk]<br />
* [/iGEM2007/index.php/Special:Preferences My preferences]<br />
* [/iGEM2007/index.php/Special:Watchlist My watchlist]<br />
* [/iGEM2007/index.php/Special:Contributions/Ian My contributions]<br />
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</div></div><div id="p-logo" class="portlet"></div><div id="p-iGEM_2007" class="portlet"><br />
<br />
=====iGEM 2007=====<br />
<br />
<div class="pBody"><br />
<br />
* [/iGEM2007/index.php/Main_Page Main Page]<br />
* [/iGEM2007/index.php/Event_Calendar About iGEM2007]<br />
* [/iGEM2007/index.php/IGEM2007_project Our project]<br />
* [/iGEM2007/index.php/Members Team Members]<br />
* [/iGEM2007/index.php/Recruitment Recruitment]<br />
* [http://gel.ym.edu.tw/~blent/symbio/reg.html Online Registration]<br />
* [/iGEM2007/index.php/Contact Contact Us]<br />
* [/iGEM2007/index.php/NYMU_iGEM2007:Site_support Sponsorship]<br />
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</div></div><div id="p-Simply_Must_Know" class="portlet"><br />
<br />
=====Simply Must Know=====<br />
<br />
<div class="pBody"><br />
<br />
* [http://partsregistry.org/cgi/htdocs/SBC04/index.cgi iGEM2004]<br />
* [http://parts2.mit.edu/wiki/index.php/Igem_2005 iGEM2005]<br />
* [/iGEM2007/index.php/IGEM2006 iGEM2006]<br />
* [/iGEM2007/index.php/Synthetic_Biology Synthetic Biology]<br />
* [/iGEM2007/index.php/FAQs FAQs]<br />
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=====Search=====<br />
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=====Toolbox=====<br />
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* [/iGEM2007/index.php/Special:Whatlinkshere/IGEM_2007_at_NYMU%2C_Taiwan What links here]<br />
* [/iGEM2007/index.php/Special:Recentchangeslinked/IGEM_2007_at_NYMU%2C_Taiwan Related changes]<br />
* [/iGEM2007/index.php/Special:Upload Upload file]<br />
* [/iGEM2007/index.php/Special:Specialpages Special pages]<br />
* [/iGEM2007/index.php?title=IGEM_2007_at_NYMU%2C_Taiwan&printable=yes Printable version]<br />
* [/iGEM2007/index.php?title=IGEM_2007_at_NYMU%2C_Taiwan&oldid=2174 Permanent link]<br />
<br />
</div></div></div></div></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:16:44Z
<p>Yfchang: </p>
<hr />
<div><font size="4" color="#ff0000">'''Latest News<br />'''</font><br />
<br />
* <font size="3" color="#000000" face="Verdana"><font face="Arial">[http://www.syntheticbiology3.ethz.ch/monday.htm Videos of the talks of "Synthetic Biology 3.0" conference] are now available.<br /><br /></font></font><br />
* <font size="3" color="#000000" face="Verdana"><font face="Arial">Take a look at the [http://pub.ucsf.edu/today/cache/feature/200708022.html UCSF Summer Assignment: Genetically Engineer a New Cell].</font></font><br />
<br />
<br /><br />
* <font size="4" color="#993366" face="Verdana">'''Our NYMU iGEM team meets regularly every week.<br />'''<font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><font size="2">[[Image:cry_smile.gif]]'' no matter if you are a formal member of our team or ''</font><font size="2"><font color="#000000">''not''.<br /></font><br /> The regular meeting for our team is scheduled as follows: <br />'''<font color="#009900">Time & Date:</font>''' 6:00 pm on every <font color="#0000ff">'''Friday'''</font><br />'''<font color="#009900">Place:</font>''' Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font>[http://www.ym.edu.tw/map.html <font size="2">check map here!</font>]<font size="2"> We are at the P3 location on the map)</font><br />
<br />
----<br />
<br />
<font size="4" color="#0000ff">'''The Beauty of our School - [http://www.ym.edu.tw/ National Yang Ming University]<br />'''</font><br />
<br />
{|<br />
|<br />
[[Image:P04.jpg]]<br />
|<br />
[[Image:P03.jpg]]<br />
|-<br />
|<br />
[[Image:Y02.jpg]]<br />
|<br />
[[Image:Y09.jpg]]<br />
|}<br />
<br />
----<br />
<br />
<font size="4"> <font color="#0000ff">'''Our iGEM2007 Mascot and Recruitment Poster ([http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg download full size version])<br />'''</font></font><br />
<br />
[[Image:Image_logo.jpg|logo]] [[Image:Image_poster.jpg|poster]]</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:08:54Z
<p>Yfchang: </p>
<hr />
<div><p><font size="4" color="#ff0000"><strong>Latest News<br /><br />
</strong></font></p><br />
<ul><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial"><a href="http://www.syntheticbiology3.ethz.ch/monday.htm">Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference</a> are now available.<br /><br />
<br /><br />
</font></font></li><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the <a href="http://pub.ucsf.edu/today/cache/feature/200708022.html">UCSF Summer Assignment: Genetically Engineer a New Cell</a>.</font></font>&nbsp;&nbsp;</li><br />
</ul><br />
<ul><br /><br />
<li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /><br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><br />
<font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /><br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /><br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /><br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font><a href="http://www.ym.edu.tw/map.html"><font size="2">check map here!</font></a><font size="2"> We are at the P3 location on the map)</font>&nbsp;</li><br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - <a href="http://www.ym.edu.tw/">National Yang Ming University</a><br /><br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" /></td><br />
</tr><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" /></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster (<a href="http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg">download full size version</a>)<br /><br />
</strong></font></font></p><br />
<p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" /> <img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" />&nbsp;</p></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:08:38Z
<p>Yfchang: </p>
<hr />
<div>Latest News<br />
<br />
* Videos of the talks of "Synthetic Biology 3.0" conference are now available.<br />
<br />
* Take a look at the UCSF Summer Assignment: Genetically Engineer a New Cell. <br />
<br />
<br />
* Our NYMU iGEM team meets regularly every week.<br />
You are more than welcome to join us for the discussion.<br />
no matter if you are a formal member of our team or not.<br />
<br />
The regular meeting for our team is scheduled as follows: <br />
Time & Date: 6:00 pm on every Friday <br />
Place: Room 417, Library/Information Building (圖資大樓), National Yang Ming University (check map here! We are at the P3 location on the map) <br />
<br />
The Beauty of our School - National Yang Ming University<br />
<br />
<br />
<br />
Our iGEM2007 Mascot and Recruitment Poster (download full size version)<br />
<br />
logo poster</div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:05:10Z
<p>Yfchang: </p>
<hr />
<div></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:03:10Z
<p>Yfchang: </p>
<hr />
<div><p><font size="4" color="#ff0000"><strong>Latest News<br /></strong></font></p><ul><li><font size="3" face="Verdana" color="#000000"><font face="Arial"><a href="http://www.syntheticbiology3.ethz.ch/monday.htm">Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference</a> are now available.<br /><br /></font></font></li><li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the <a href="http://pub.ucsf.edu/today/cache/feature/200708022.html">UCSF Summer Assignment: Genetically Engineer a New Cell</a>.</font></font>&nbsp;&nbsp;</li></ul><ul><br /><li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /></strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /></font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /><strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /><strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font><a href="http://www.ym.edu.tw/map.html"><font size="2">check map here&#33;</font></a><font size="2"> We are at the P3 location on the map)</font>&nbsp;</li></ul><hr /><p><font size="4" color="#0000ff"><strong>The Beauty of our School - <a href="http://www.ym.edu.tw/">National Yang Ming University</a><br /></strong></font></p><br />
{| <tbody> <br />
| <img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" /><br />
| <img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" /><br />
|- <br />
| <img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" /><br />
| <img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" /></tbody><br />
|}<hr /><p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster (<a href="http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg">download full size version</a>)<br /></strong></font></font></p><p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" /><img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" />&nbsp;</p></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:02:50Z
<p>Yfchang: </p>
<hr />
<div></div>
Yfchang
http://2007.igem.org/wiki/index.php/NYMU_Taipei/Welcome
NYMU Taipei/Welcome
2007-10-18T01:02:32Z
<p>Yfchang: </p>
<hr />
<div><p><font size="4" color="#ff0000"><strong>Latest News<br /><br />
</strong></font></p><br />
<ul><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial"><a href="http://www.syntheticbiology3.ethz.ch/monday.htm">Videos of the talks of &quot;Synthetic Biology 3.0&quot; conference</a> are now available.<br /><br />
<br /><br />
</font></font></li><br />
<li><font size="3" face="Verdana" color="#000000"><font face="Arial">Take a look at the <a href="http://pub.ucsf.edu/today/cache/feature/200708022.html">UCSF Summer Assignment: Genetically Engineer a New Cell</a>.</font></font>&nbsp;&nbsp;</li><br />
</ul><br />
<ul><br /><br />
<li><font size="4" face="Verdana" color="#993366"><strong>Our NYMU iGEM team meets regularly every week.<br /><br />
</strong><font size="3" color="#ff0000">You are more than welcome to join us for the discussion.</font></font><br /><br />
<font size="2"><img src="/iGEM2007/FCKeditor/editor/images/smiley/msn/cry_smile.gif" alt="" /><em>&nbsp;no matter if you&nbsp;are a formal member of&nbsp;our team&nbsp;or </em></font><font size="2"><font color="#000000"><em>not</em>.<br /><br />
</font>&nbsp; <br /><br />
The regular meeting for our team is scheduled as follows:&nbsp;<br /><br />
<strong><font color="#009900">Time &amp; Date:</font></strong> 6:00&nbsp;pm on every&nbsp;<font color="#0000ff"><strong>Friday</strong></font><strong>&nbsp;</strong><br /><br />
<strong><font color="#009900">Place:</font></strong> Room <font color="#ff0000">417</font>, Library/Information Building (圖資大樓), National Yang Ming University (</font><a href="http://www.ym.edu.tw/map.html"><font size="2">check map here!</font></a><font size="2"> We are at the P3 location on the map)</font>&nbsp;</li><br />
</ul><br />
<hr /><br />
<p><font size="4" color="#0000ff"><strong>The Beauty of our School - <a href="http://www.ym.edu.tw/">National Yang Ming University</a><br /><br />
</strong></font></p><br />
<table><br />
<tbody><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/a/a0/P04.jpg" alt="" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/3/37/P03.jpg" alt="" /></td><br />
</tr><br />
<tr><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/1/13/Y02.jpg" alt="" /></td><br />
<td><img src="http://igem.ym.edu.tw/iGEM2007/images/d/d3/Y09.jpg" alt="" /></td><br />
</tr><br />
</tbody><br />
</table><br />
<hr /><br />
<p><font size="4">&nbsp;<font color="#0000ff"><strong>Our iGEM2007 Mascot and Recruitment Poster (<a href="http://igem.ym.edu.tw/iGEM2007/images/a/af/Poster_large_full.jpg">download full size version</a>)<br /><br />
</strong></font></font></p><br />
<p><img src="http://igem.ym.edu.tw/iGEM2007/images/5/53/Image_logo.jpg" alt="logo" /> <img src="http://igem.ym.edu.tw/iGEM2007/images/5/55/Image_poster.jpg" alt="poster" />&nbsp;</p></div>
Yfchang