From 2007.igem.org
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| - | PCR otsAF/otsAIR on otsA (413 bp, gp=A)
| + | [[otsAconstructionfile]] |
| - | PCR otsAIF/otsAR on otsA (1077 bp, gp=B)
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| - | --------------------------------------------
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| - | PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI)
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| - | Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)
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| - | Product pBca9145-BBa I716301
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| - | --------------------------------------------
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| - | otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC
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| - | otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
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| - | otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
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| - | otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC
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Revision as of 22:02, 28 June 2007
otsAconstructionfile
