Tokyo/AHL assay Standard protocol

From 2007.igem.org

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(→AHL assay Standard protocol)
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<br>take 30 ul of the overnight culture into LB + Kan (⇒fresh culture)
<br>take 30 ul of the overnight culture into LB + Kan (⇒fresh culture)
<br>incubate the fresh culture until the observed OD reaches around 0.80.
<br>incubate the fresh culture until the observed OD reaches around 0.80.
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<br>prepare and add AHL mixture; final concentration of AHL = 6 pM
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<br>prepare and add AHL mixture<br>incubation for 2 hours  
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<br>incubation for 2 hours  
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<br>FLA measurements for GFP expression
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<br>1st FLA measurement for GFP expression
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<br>2nd FLA measurement for GFP expression
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<br>add the same amout AHL as the first addition
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Revision as of 13:50, 20 October 2007

AHL assay Standard protocol


prepare overnight culture (3 tubes for luxR, 2 tubes for each controls)
take 30 ul of the overnight culture into LB + Kan (⇒fresh culture)
incubate the fresh culture until the observed OD reaches around 0.80.
prepare and add AHL mixture
incubation for 2 hours
FLA measurements for GFP expression

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