Tokyo/AHL assay Standard protocol
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== AHL assay Standard protocol == | == AHL assay Standard protocol == | ||
| - | <br>prepare overnight culture | + | <br>prepare overnight culture |
| - | <br> | + | <br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) |
<br>take 30 ul of the overnight culture into LB + Kan (⇒fresh culture) | <br>take 30 ul of the overnight culture into LB + Kan (⇒fresh culture) | ||
<br>incubate the fresh culture until the observed OD reaches around 0.80. | <br>incubate the fresh culture until the observed OD reaches around 0.80. | ||
<br>prepare and add AHL mixture<br>incubation for 2 hours | <br>prepare and add AHL mixture<br>incubation for 2 hours | ||
<br>FLA measurements for GFP expression | <br>FLA measurements for GFP expression | ||
Revision as of 13:52, 20 October 2007
AHL assay Standard protocol
prepare overnight culture
(3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control)
take 30 ul of the overnight culture into LB + Kan (⇒fresh culture)
incubate the fresh culture until the observed OD reaches around 0.80.
prepare and add AHL mixture
incubation for 2 hours
FLA measurements for GFP expression
