Tokyo/AHL assay Standard protocol
From 2007.igem.org
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(→AHL assay Standard protocol) |
(→AHL assay Standard protocol) |
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<br>prepare overnight culture | <br>prepare overnight culture | ||
<br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) | <br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) | ||
- | <br>take 30 ul of the overnight culture into LB + | + | <br>take 30 ul of the overnight culture into LB + Antibiotics (⇒fresh culture) |
<br>incubate the fresh culture until the observed OD reaches around 0.80. | <br>incubate the fresh culture until the observed OD reaches around 0.80. | ||
<br>prepare and add AHL mixture<br>incubation for 2 hours | <br>prepare and add AHL mixture<br>incubation for 2 hours | ||
<br>FLA measurements for GFP expression | <br>FLA measurements for GFP expression |
Revision as of 15:58, 20 October 2007
AHL assay Standard protocol
prepare overnight culture
(3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control)
take 30 ul of the overnight culture into LB + Antibiotics (⇒fresh culture)
incubate the fresh culture until the observed OD reaches around 0.80.
prepare and add AHL mixture
incubation for 2 hours
FLA measurements for GFP expression