Tokyo/AHL assay Standard protocol
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== AHL assay Standard protocol == | == AHL assay Standard protocol == | ||
| - | [[Image:AHLstandard.jpg|500px]] | + | [[Image:AHLstandard.jpg|thum|500px|'''Fig. 1. AHL Assay ''' <br>Basically, experiments using AHL employed this process.]] |
<br>prepare overnight culture | <br>prepare overnight culture | ||
<br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) | <br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) | ||
Revision as of 13:48, 25 October 2007
AHL assay Standard protocol
prepare overnight culture
(3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control)
take 30 ul of the overnight culture into LB + Antibiotics (⇒fresh culture)
incubate the fresh culture until the observed OD reaches around 0.80.
prepare and add AHL mixture
incubation for 2 hours
FLA measurements for GFP expression
