Tokyo/AHL assay Standard protocol
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== AHL assay Standard protocol == | == AHL assay Standard protocol == | ||
- | [[Image:AHLstandard.jpg|thumb|500px|'''Fig. 1. AHL Assay ''' <br>Basically, experiments using AHL employed this process.]] | + | [[Image:AHLstandard.jpg|thumb|500px|'''Fig. 1. AHL Assay ''' <br>Basically, experiments using AHL employed this process. [[Tokyo/Wash |Wash]]ing process had been employed later in our project (at the middle of the preliminary assays).]] |
<br>prepare overnight culture | <br>prepare overnight culture | ||
<br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) | <br> (3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control) |
Latest revision as of 13:52, 25 October 2007
AHL assay Standard protocol
prepare overnight culture
(3 tubes for AHL-activated promoters, 2 tubes for each - positive and negative -control)
take 30 ul of the overnight culture into LB + Antibiotics (⇒fresh culture)
incubate the fresh culture until the observed OD reaches around 0.80.
prepare and add AHL mixture
incubation for 2 hours
FLA measurements for GFP expression