Tokyo/Assay

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新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める.
新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める.
ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
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We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
<br>[[Image:expression6-1.JPG|300px|]]
<br>[[Image:expression6-1.JPG|300px|]]
<br>[[Image:expression6-2.JPG|340px|]]
<br>[[Image:expression6-2.JPG|340px|]]
導出に関してはfor more detail.
導出に関してはfor more detail.
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= [[Tokyo/Preliminary assays |Preliminary assays ]] =
= [[Tokyo/Preliminary assays |Preliminary assays ]] =

Revision as of 00:05, 22 October 2007

Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる. We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
Expression6-1.JPG
Expression6-2.JPG

導出に関してはfor more detail.

Preliminary assays

Fig.1: Exogenously added AHL

Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

Assays for hybrid promoter

These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.