Tokyo/Assay
From 2007.igem.org
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+ | = [[Tokyo/Hill function fitting |Hill function fitting ]] = | ||
+ | 新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. | ||
+ | ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる. | ||
+ | |||
+ | <br>[[Image:expression6-1.JPG|300px|]] | ||
+ | <br>[[Image:expression6-2.JPG|340px|]] | ||
+ | |||
+ | 導出に関してはfor more detail. | ||
+ | |||
+ | |||
= [[Tokyo/Preliminary assays |Preliminary assays ]] = | = [[Tokyo/Preliminary assays |Preliminary assays ]] = | ||
[[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]] | [[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]] |
Revision as of 21:34, 21 October 2007
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
導出に関してはfor more detail.
Preliminary assays
Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.
Assays for hybrid promoter
These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.