Tokyo/Assay

From 2007.igem.org

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= [[Tokyo/Hill function fitting |Hill function fitting ]] =
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新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める.
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ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.
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<br>[[Image:expression6-1.JPG|300px|]]
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<br>[[Image:expression6-2.JPG|340px|]]
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導出に関してはfor more detail.
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= [[Tokyo/Preliminary assays |Preliminary assays ]] =
= [[Tokyo/Preliminary assays |Preliminary assays ]] =
[[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]]
[[Image:AHL Assay2.JPG|thumb|200px| '''Fig.1: '''Exogenously added AHL]]

Revision as of 21:34, 21 October 2007

Hill function fitting

新しく作ったハイブリッドプロモーターに関してはデータがないので,実験によりパラメータを求める. ヒル関数をfittingさせることにより,n2,n3,K2,K3が求まる.


Expression6-1.JPG
Expression6-2.JPG

導出に関してはfor more detail.


Preliminary assays

Fig.1: Exogenously added AHL

Preliminaly assays were done
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

Assays for hybrid promoter

These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.