Tokyo/Lux-lac hybrid promoter activation check
From 2007.igem.org
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| - | === | + | __NOTOC__ |
| + | ===Objective: === | ||
check if our lux-lac hybrid promoter is activated by AHL | check if our lux-lac hybrid promoter is activated by AHL | ||
===Samples: === | ===Samples: === | ||
| - | Lux-lac hybrid promoter (before blue light check) | + | Lux-lac hybrid promoter (before blue light check*) x3 samples |
| - | <br>Lux-lac hybrid promoter (after blue light check) | + | <br>Lux-lac hybrid promoter (after blue light check) x3 samples |
<br>placQI (pos. con.) | <br>placQI (pos. con.) | ||
<br>modified A4 (promoter deleted) (neg. con.) | <br>modified A4 (promoter deleted) (neg. con.) | ||
| + | |||
| + | (*Blue light check: Transformed with the hybrid promoter + GFP, the colonies should look green irradiated by blue light. Greenish colonies were picked up for the overnight culture. In addition, since this irradiation could affect the cells and their genes, colonies before the irradiation were picked up without confirming if they looked green. ) | ||
===Procedure: === | ===Procedure: === | ||
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===Result & Conclusion: === | ===Result & Conclusion: === | ||
| + | 2 out of the 3 samples of Lux-lac hybrid promoter (before blue light check*) and | ||
| + | <br>1 out of the 3 samples of Lux-lac hybrid promoter (after blue light check*) | ||
| + | <br>showed significant fluorescence; whereas, | ||
| + | <br>1 out of the 3 samples of Lux-lac hybrid promoter (before blue light check*) and | ||
| + | <br>2 out of the 3 samples of Lux-lac hybrid promoter (after blue light check*) did not. | ||
| + | <br>1 out of the 3 samples of Lux-lac hybrid promoter (before blue light check*) showed weak fluorescence. <br>(For the last sample, we detected that this sample emerged from cells from two colonies, one containing the hybrid promoter + GFP and the other not by mini-prep and electrophoresis.) | ||
== == | == == | ||
[[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | [[Tokyo/Preliminary assays |"Back to Preliminary assays"]] | ||
Latest revision as of 18:49, 26 October 2007
Objective:
check if our lux-lac hybrid promoter is activated by AHL
Samples:
Lux-lac hybrid promoter (before blue light check*) x3 samples
Lux-lac hybrid promoter (after blue light check) x3 samples
placQI (pos. con.)
modified A4 (promoter deleted) (neg. con.)
(*Blue light check: Transformed with the hybrid promoter + GFP, the colonies should look green irradiated by blue light. Greenish colonies were picked up for the overnight culture. In addition, since this irradiation could affect the cells and their genes, colonies before the irradiation were picked up without confirming if they looked green. )
Procedure:
Result & Conclusion:
2 out of the 3 samples of Lux-lac hybrid promoter (before blue light check*) and
1 out of the 3 samples of Lux-lac hybrid promoter (after blue light check*)
showed significant fluorescence; whereas,
1 out of the 3 samples of Lux-lac hybrid promoter (before blue light check*) and
2 out of the 3 samples of Lux-lac hybrid promoter (after blue light check*) did not.
1 out of the 3 samples of Lux-lac hybrid promoter (before blue light check*) showed weak fluorescence.
(For the last sample, we detected that this sample emerged from cells from two colonies, one containing the hybrid promoter + GFP and the other not by mini-prep and electrophoresis.)
