Tokyo/Lux-lac hybrid promoter activation check
From 2007.igem.org
Contents |
Purpose:
check if our lux-lac hybrid promoter is activated by AHL
Samples:
Lux-lac hybrid promoter (before blue light check*) x3 samples
Lux-lac hybrid promoter (after blue light check) x3 samples
placQI (pos. con.)
modified A4 (promoter deleted) (neg. con.)
(*Blue light check: Transformed with the hybrid promoter + GFP, the colonies should look green irradiated by blue light. Greenish colonies were picked up for the overnight culture. In addition, since this irradiation could affect the cells and their genes, colonies before the irradiation were picked up without confirming if they looked green. )
