Tokyo/New AHL check

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Contents

Purpose:


To determine and compare the activity of the AHL used so far and that of the newly perchased AHL.

Samples:


A4 placQI in pTrc99A (pos. con.)
A4ΔP in pTrc99A (neg. con.)
A4 hybrid promoter in pBR322

Procedure:


The old and the newly delivered AHL was used.
AHL assay Standard protocol
The final concantrations of AHL applied here were 0.01, 0.1, 0.5, 1, 10, 100, and 1000 nM.

Result & Conclusion: