Tokyo/Wash

From 2007.igem.org

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== Wash ==
== Wash ==
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[[Image:wash1.jpg]]
<br>take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
<br>take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
<br>centrifugation for 2 min at 9000g
<br>centrifugation for 2 min at 9000g
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<br>mix well
<br>mix well
<br>apply 150 ml onto a well
<br>apply 150 ml onto a well
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== ==
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[[Tokyo/Preliminary assays |"Back to Preliminary assays"]]

Latest revision as of 07:09, 25 October 2007

Wash

Wash1.jpg
take 200 ml of culture in a Falcon tube into a eppendorf tube (1.6 or 2.0 ml tube)
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
centrifugation for 2 min at 9000g
discard the supernatant with a pippet
add 200 ml of PBS and dissolve the pellet
mix well
apply 150 ml onto a well

"Back to Preliminary assays"