Tokyo/Works/Assay2
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<br>Fomulationで建てた式に必要なパラメータをAssay１により実験的に求めた。それを導入した結果を解析したところ、以下の実験の必要性が浮かび上がった。 | <br>Fomulationで建てた式に必要なパラメータをAssay１により実験的に求めた。それを導入した結果を解析したところ、以下の実験の必要性が浮かび上がった。 | ||
| - | == | + | == [[Tokyo/Activation check by cell-produced AHL |Activation check by cell-produced AHL ]] == |
==== Purpose ==== | ==== Purpose ==== | ||
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| - | + | To check if worker E. coli (Sender) can produce enough AHL for our model to work by using different copy numbers of plasmids. | |
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====Result & Conclusion==== | ====Result & Conclusion==== | ||
| + | [[Image:AHL_assay_endogenous_Results.jpg]] | ||
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| + | <br>Not only high copy number plasmid pSB1, also low copy number plasmid pSB4 and pBR produced enough AHL to activate the LacI hybrid promoter in other cells. Especially, pBR remarkably produced AHL in the present experiment. | ||
==Comparison of promoter activity== | ==Comparison of promoter activity== | ||
==== Purpose ==== | ==== Purpose ==== | ||
====Result & Conclusion==== | ====Result & Conclusion==== | ||
Revision as of 02:50, 24 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Fomulationで建てた式に必要なパラメータをAssay１により実験的に求めた。それを導入した結果を解析したところ、以下の実験の必要性が浮かび上がった。
Activation check by cell-produced AHL
Purpose
To check if worker E. coli (Sender) can produce enough AHL for our model to work by using different copy numbers of plasmids.
Result & Conclusion
Not only high copy number plasmid pSB1, also low copy number plasmid pSB4 and pBR produced enough AHL to activate the LacI hybrid promoter in other cells. Especially, pBR remarkably produced AHL in the present experiment.
