Tokyo/Works/Assay2

From 2007.igem.org

Works top   0.Hybrid promoter   1.Formulation   2.Assay1   3.Simulation   4.Assay2   5.Future works

Activation check by cell-produced AHL    Expression level check on promoters + plasmid sets of A and B sides

Objective

Parameters for the equations in Formulation have been experimentally determined in Assay1. Analysing the result of the simulations, the following experiments were turned out to be necessary.

Activation check by cell-produced AHL

Fig.1 By culturing with AHL producing cells, whether the lux lac hybrid promoter was activated without externally adding AHL. Regardless of the copy numbers of the luxI encoding plasmids, the hybrid promoter was activated.

Objective

To check whether worker E. coli (Sender) can produce enough amount of AHL for our model to work by using different copy numbers of plasmids

Result & Conclusion

Not only high copy number plasmid pSB1, but also low copy number plasmid pSB4 and pBR produced enough AHL to activate the LacI hybrid promoter in other cells. Especially, pBR remarkably produced AHL in the present experiment.

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