Toronto/Lab Notebook

From 2007.igem.org

(Difference between revisions)
Line 17: Line 17:
* Transform:  
* Transform:  
** J06801 (2005) - it's in the source box,  
** J06801 (2005) - it's in the source box,  
-
** B0034 - also in  
+
** B0034 - also in source box, if not, get it from Registry plates
 +
** Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
 +
* Ligate N1 + S01003
== Oct. 1, 2007 ==
== Oct. 1, 2007 ==

Revision as of 15:28, 3 October 2007

< August | September | Current >

Oct. 2, 2007

Natalie

SUMMARY:

  • Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
  • NOTE: N1 = J34100 C + S01640 B + B0015

TO DO:

  • Miniprep all above parts and length check
  • Transform:
    • J06801 (2005) - it's in the source box,
    • B0034 - also in source box, if not, get it from Registry plates
    • Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
  • Ligate N1 + S01003

Oct. 1, 2007

Time: 3:45 PM - 8:30 PM

Yusuf, Irina

Summary:

  • made 5 KAN plates
  • made 2 AMP+KAN plates
  • made 300 mL LB

Tranformation of following parts:

  • I13033 -> KAN
  • E0433 -> KAN
  • S03140 -> AMP
  • J04500 -> KAN
  • J31003 -> AMP
  • (J23100C + S01640B)+ B0015 -> KAN

Used 4uL of DNA for all transformations

  • tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
  • couldn't find it in the igem dna box as well !!!

TO DO:

  • MP o/n for the above parts