Toronto/Lab Notebook

From 2007.igem.org

(Difference between revisions)
(Oct. 3, 2007)
Line 12: Line 12:
* Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
* Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
* Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
* Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
-
* Length Check (o = match, x = does not match):
+
* Length Check (O = match, X = does not match):
<table border=1>
<table border=1>
   <tr><td>'''Part Name'''<td>'''Part'''<td>'''Plasmid'''</tr>
   <tr><td>'''Part Name'''<td>'''Part'''<td>'''Plasmid'''</tr>
-
   <tr><td>E0433 A<td><td></tr>
+
   <tr><td>E0433 A<td>O<td>O</tr>
-
   <tr><td>E0433 B<td><td></tr>
+
   <tr><td>E0433 B<td>O<td>O</tr>
-
   <tr><td>I13033 A<td><td></tr>
+
   <tr><td>I13033 A<td>X<td>O</tr>
-
   <tr><td>I13033 B<td><td></tr>
+
   <tr><td>I13033 B<td>X<td>O</tr>
-
   <tr><td>J04500 A<td><td></tr>
+
   <tr><td>J04500 A<td>O<td>O</tr>
-
   <tr><td>J04500 B<td><td></tr>
+
   <tr><td>J04500 B<td>O<td>O</tr>
-
   <tr><td>J31003 A<td><td></tr>
+
   <tr><td>J31003 A<td>O<td>O</tr>
-
   <tr><td>J31003 B<td><td></tr>
+
   <tr><td>J31003 B<td>O<td>O</tr>
-
   <tr><td>N1     A<td><td></tr>
+
   <tr><td>N1 A<td>O<td>O</tr>
-
   <tr><td>N1     B<td><td></tr>
+
   <tr><td>N1 B<td>O<td>O</tr>
-
   <tr><td>S03140 A<td><td></tr>
+
   <tr><td>S03140 A<td>O<td>O</tr>
-
   <tr><td>S03140 B<td><td></tr>
+
   <tr><td>S03140 B<td>O<td>O</tr>
</table>
</table>

Revision as of 01:51, 4 October 2007

< August | September | Current >

Oct. 3, 2007

Yusuf

SUMMARY:

  • Transformed: J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
  • Minipreped I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
  • Made o/n of DH5a and DH5a-z1 (from July 16 plate - ask Seema if this is ok)
  • Length Check (O = match, X = does not match):
Part NamePartPlasmid
E0433 AOO
E0433 BOO
I13033 AXO
I13033 BXO
J04500 AOO
J04500 BOO
J31003 AOO
J31003 BOO
N1 AOO
N1 BOO
S03140 AOO
S03140 BOO

TO DO:

  • Make 5 Kan plates
  • Make bottle of LB Broth
  • Make competent cells
  • Make o/n of J06801 (2005), Q04400 (2005), B0034 (2007), J31005 (2007)
  • Ligate N1 + S01003

Oct. 2, 2007

Natalie

SUMMARY:

  • Made o/n (2 each) of: I130033 AB, E0433 AB, S03140 AB, J04500 AB, J31003 AB, N1 AB
  • NOTE: N1 = J34100 C + S01640 B + B0015

TO DO:

  • Miniprep all above parts and length check
  • Transform:
    • J06801 (2005) - it's in the source box (this is a larger composite part containing J06501)
    • B0034 - also in source box, if not, get it from Registry plates
    • Q04400 - re-transform using more DNA to see if we get more colonies and transform 2005/2006 version in the source box
    • J31005 - chloramphenicol resistance, just in case we need it
  • Continue cataloging and organization (Charles: I'll be dropping by to help out with that)

Oct. 1, 2007

Time: 3:45 PM - 8:30 PM

Yusuf, Irina

Summary:

  • made 5 KAN plates
  • made 2 AMP+KAN plates
  • made 300 mL LB

Tranformation of following parts:

  • I13033 -> KAN
  • E0433 -> KAN
  • S03140 -> AMP
  • J04500 -> KAN
  • J31003 -> AMP
  • (J23100C + S01640B)+ B0015 -> KAN

Used 4uL of DNA for all transformations

  • tried to do DNA extraction for J06501 but somebody already extracted it (wasn't in the well)
  • couldn't find it in the igem dna box as well !!!

TO DO:

  • MP o/n for the above parts