USTC

From 2007.igem.org

(Difference between revisions)
m
 
(174 intermediate revisions not shown)
Line 1: Line 1:
__NOTOC__
__NOTOC__
-
[[Image:USTC\Logo.jpg|640px]]
 
-
[[Image:DSCN1243.png|512px]]
+
[[Image:USTC_Logo.png|512px]]
-
''From left to right''<BR>
+
<!-- <font color="red">
-
''Back row:'' [[USTC/ZhaoYun|Zhao Yun]], [[USTC/LiuZiqing|Liu Ziqing]], [[USTC/MaXiaoyu|Ma Xiaoyu]]<BR>
+
There are'''<html><script language="JAVASCRIPT"><!-- update your browser, silly-->
-
''Front row:'' [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su Xiaofeng]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
+
Today = new Date();
 +
Jamboree = new Date("November 3, 2007");
 +
msInADay = 1000 * 60 * 60 * 24;
 +
display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay);
 +
document.write(" " + display +" ");
 +
// </script></html>'''days left until the Jamboree!
 +
</font> -->
 +
----
 +
{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
 +
|-valign="top"
 +
|width=400px style="padding: 5px" |
 +
=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
 +
Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit <i>in vivo</i> with several levels of gates.
-
----
+
Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation <i>in vivo</i>, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.
-
=== Our Project: Enlargeable Logic Circuit in Bacteria ===
+
A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?
-
Artificial Cell Signaling Network (ACS Network) and Integrated Circuit Network (IC Network) are both signaling networks. Now we are enjoying the advantage of Ultra Large Scale IC (ULSI), but at least today we cannot implement a somewhat large ACS Network in actual experiments.
+
<font color="#AAAAAA">''For detailed description, please click each hyperlink below.''</font>
-
Our project is to provide a new viewpoint for building up fully extensible ACS Networks. We are going to produce biologic logic gates such as NAND, NOR, NOT for some biological conveniences. In other words, modern logic IC Networks are built upon these three gates-- NAND, NOR and NOT. Logic gates are only the basic elements of logic IC, on the other hand, the more important factors we think which make IC Network to be easily enlarged are: Reducing the scale of elements; Tuning elements to eliminate interference; Calibrating elements for interconnection. Cis-acting elements may provide smaller scale than trans-acting ones, while the other two factors can be achieved by standardization. We will present the method for standardization and demonstrate an ACS Network made by these biologic logic gates.
+
-
This ACS Network may not be large, but the important factors we implement in it will naturally make it fully extensible. IC has been born for 50 years and how hugely it impacts our modern life! So, what about biologic logic circuit in next 50 years? A new world is waiting...
+
 +
|width=330px style="padding: 5px" |
-
{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
+
[[Image:DSCN1243.png|330px]]
-
|-valign="top"
+
-
|width=240px style="padding: 5px|
+
-
<h3>Events</h3>
+
<font color="#AAAAAA">''From left to right''</font><BR>
 +
<font color="#AAAAAA">''Back row:''</font> [[USTC/ZhaoYun|Zhao Yun]], [[USTC/LiuZiqing|Liu ZQ]], [[USTC/MaXiaoyu|Ma XY]]<BR>
 +
<font color="#AAAAAA">''Front row:''</font> [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su XF]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
-
'''01/08/2007'''
+
<BR clear="both">
 +
<center><html>
 +
<script type="text/javascript">
 +
function cantload() {
 +
img = document.getElementById("clustrMapsImg");
 +
img.onerror = null;
 +
img.src = "http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg";
 +
document.getElementById("clustrMapsLink").href = "http://www2.clustrmaps.com";
 +
}
 +
img = document.getElementById("clustrMapsImg");
 +
img.onerror = cantload;
 +
</script>
 +
</html></center>
 +
|}
-
There is a mistake in my preparing X-Gal solution. Please rework your correlative experiments. Sorry!
+
{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
 +
|-valign="top"
 +
|width=240px style="padding: 5px; background-color: #ccccff" |
-
( by Ma Rui )
+
<h3>Project</h3>
-
'''30/07/2007'''
 
-
First designed repressors. Good job, Bo and Xiaofeng!
+
[[USTC/Introduction|Introduction]]
-
'''01/06/2007'''
 
-
Primary experiments begin around this day.
+
'''Core Logic Components:'''
-
[[USTC/Previous|'''Previous''']]
+
[[USTC/Logic-Gate_Promoters|Logic-Gate Promoters]]
-
|width=240px style="padding: 5px|
 
-
<h3>Tasks</h3>
+
'''Wires without Interference:'''
-
[[USTC/PoPSConvertors|PoPS Convertors]]
+
[[USTC/Repressor_Evolution_on_Plates|Repressor Evolution on Plates]]
-
[[USTC/LogicGates|Biologic Logic Gates]]
+
[[USTC/Repressor_Evolution_in_Silico|Repressor Evolution <i>in Silico</i>]]
-
[[USTC/RepressorDesigning|Redesign the Lac Repressor]]
 
-
[[USTC/PoPSReporter|Fluorescent Reporters]]
+
'''Peripheral Devices:'''
-
[[USTC/OverallSystem|Link All Things Together]]
+
[[USTC/Inputs_and_Outputs|Inputs and Outputs]]
-
<!-- [[USTC/XORGate|A simpler design for an XOR gate]] -->
 
-
<!-- [[USTC/ANDGate|An AND gate]] -->
+
'''Extensible System:'''
-
<!-- [[USTC/BiobricksManual|A BioBricks Manual in Chinese]] -->
+
[[USTC/Demonstration|A Demonstration]]
 +
[[USTC/Further_More|Further More]]
-
|width=240px style="padding: 5px|
+
 
 +
|width=240px style="padding: 5px; background-color: #ccff99" |
<h3>Members</h3>
<h3>Members</h3>
 +
<center>
-
'''Graduates:'''<BR>
+
 
 +
'''4 Graduates:'''<BR>
[[USTC/ZhanJian|Zhan Jian]]<BR>
[[USTC/ZhanJian|Zhan Jian]]<BR>
-
[[USTC/DingBo|Ding Bo]]<BR>
 
[[USTC/MaRui|Ma Rui]]<BR>
[[USTC/MaRui|Ma Rui]]<BR>
 +
[[USTC/DingBo|Ding Bo]]<BR>
[[USTC/MaXiaoyu|Ma Xiaoyu]]<BR>
[[USTC/MaXiaoyu|Ma Xiaoyu]]<BR>
-
'''Undergraduates:'''<BR>
+
 
 +
 
 +
'''3 Undergraduates:'''<BR>
[[USTC/SuXiaofeng|Su Xiaofeng]]<BR>
[[USTC/SuXiaofeng|Su Xiaofeng]]<BR>
[[USTC/LiuZiqing|Liu Ziqing]]<BR>
[[USTC/LiuZiqing|Liu Ziqing]]<BR>
[[USTC/ZhaoYun|Zhao Yun]]
[[USTC/ZhaoYun|Zhao Yun]]
-
'''Faculty Advisors:'''<BR>
+
 
 +
 
 +
'''3 Faculty Advisors:'''<BR>
Prof. HY Liu<BR>
Prof. HY Liu<BR>
Prof. JR Wu<BR>
Prof. JR Wu<BR>
Prof. ZH Hou
Prof. ZH Hou
 +
</center>
 +
 +
|width=240px style="padding: 5px; background-color: #FFFF99" |
 +
 +
<h3>Snapshot</h3>
 +
 +
 +
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC '''<font color="#bb0000">247</font> Part Sequences''']
 +
 +
'''<font color="#bb0000">123</font> Parts Submitted'''
 +
 +
 +
'''77''' Synthesized Promoters
 +
 +
'''11''' Novel Artificial Repressors
 +
 +
 +
'''~ 350''' New Strains
 +
 +
'''~ 130''' DNA Strands Sequenced
 +
 +
'''> 5000''' Colonies Screened
 +
 +
'''~ 400''' Quantitative Assays
 +
 +
 +
 +
 +
<br>
 +
<br>
 +
<br>
 +
[[USTC/Events|'''Previous Events''']]
|-valign="top"
|-valign="top"
-
|width=240px style="padding: 5px|
+
|width=240px style="padding: 5px; background-color: #ccff99"|
<h3>Resources</h3>
<h3>Resources</h3>
Line 90: Line 150:
[[USTC/Protocols|Protocols]]
[[USTC/Protocols|Protocols]]
-
[[USTC/PromoterDesign|Promoter Design]]
+
[[USTC/Sponsors|Sponsors]]
[[USTC/ServiceProviders|Service Providers]]
[[USTC/ServiceProviders|Service Providers]]
-
|width=240px style="padding: 5px|
+
|width=240px style="padding: 5px; background-color: #FFFF99"|
<h3>Gallery</h3>
<h3>Gallery</h3>
Line 106: Line 166:
[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]
[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]
-
|width=240px style="padding: 5px|
+
|width=240px style="padding: 5px; background-color: #ccccff"|
<h3>Links</h3>
<h3>Links</h3>
-
[http://partsregistry.org iGEM standard parts' catalog ]
+
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]
-
[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]
+
[http://partsregistry.org iGEM Standard Parts' Catalog ]
-
[http://groups.google.com/group/iGEMChina iGEM China Group]
+
[[IGEM2007 Team List]]
 +
 
 +
[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]
|}
|}
 +
 +
----

Latest revision as of 10:51, 8 March 2011


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->


Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.

A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?

For detailed description, please click each hyperlink below.

DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project


Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Members


4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun


3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Snapshot


247 Part Sequences

123 Parts Submitted


77 Synthesized Promoters

11 Novel Artificial Repressors


~ 350 New Strains

~ 130 DNA Strands Sequenced

> 5000 Colonies Screened

~ 400 Quantitative Assays






Previous Events

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

USTC Photos

Huangshan Mountain

Links

Parts Made by USTC iGEM 2007

iGEM Standard Parts' Catalog

IGEM2007 Team List

Univ. of Sci. and Tech. of China