http://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&feed=atom&action=historyUSTC/HybridOperator - Revision history2024-03-29T01:43:26ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48571&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T06:43:08Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be strong. Now it is clear that asymmetric O24 can be bound to both by R2 and R4, but neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. <del class="diffchange diffchange-inline">Now we </del>can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be strong. Now it is clear that asymmetric O24 can be bound to both by R2 and R4, but neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. <ins class="diffchange diffchange-inline">We </ins>can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48567&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T06:32:57Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be strong. <del class="diffchange diffchange-inline">It </del>is clear that asymmetric O24 can be bound to both by R2 and R4<del class="diffchange diffchange-inline">. However</del>, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be strong. <ins class="diffchange diffchange-inline">Now it </ins>is clear that asymmetric O24 can be bound to both by R2 and R4, <ins class="diffchange diffchange-inline">but </ins>neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48558&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T06:07:39Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be <del class="diffchange diffchange-inline">that </del>strong. It is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be strong. It is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48555&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T06:05:41Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22<del class="diffchange diffchange-inline">, well then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa</del>. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind on half strand could still maintain repression, but distinctly it could no longer be that strong. <ins class="diffchange diffchange-inline">It is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. </ins>Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48546&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T05:58:58Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind <del class="diffchange diffchange-inline">to </del>half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind <ins class="diffchange diffchange-inline">on </ins>half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48312&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T04:57:54Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well<del class="diffchange diffchange-inline">, </del>then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole, heterodimer R2~R4 can bind on O24 as tightly as R2+O22 or R4+O44.</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48295&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T04:55:55Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole heterodimer R2~R4 can bind on O24 as <del class="diffchange diffchange-inline">tight </del>as R2+O22 or R4+O44.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well. On the whole<ins class="diffchange diffchange-inline">, </ins>heterodimer R2~R4 can bind on O24 as <ins class="diffchange diffchange-inline">tightly </ins>as R2+O22 or R4+O44.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48278&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T04:54:07Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Furthermore, the dimerization regions of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 binds on the right half as well<ins class="diffchange diffchange-inline">. On the whole heterodimer R2~R4 can bind on O24 as tight as R2+O22 or R4+O44</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48262&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T04:51:27Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Furthermore, the dimerization <del class="diffchange diffchange-inline">region </del>of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 <del class="diffchange diffchange-inline">bind </del>on the right half as well.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Furthermore, the dimerization <ins class="diffchange diffchange-inline">regions </ins>of our artificial repressors are uniform, so they can combine into the heterodimeric form, for example, heterodimer R2~R4. Its one side of R2 can bind to O24's left-half strand specifically while the other side of R4 <ins class="diffchange diffchange-inline">binds </ins>on the right half as well.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td></tr>
</table>MaRuihttp://2007.igem.org/wiki/index.php?title=USTC/HybridOperator&diff=48250&oldid=prevMaRui: /* Hybrid Operators for Heterodimeric Repressor */2007-10-27T04:49:21Z<p><span class="autocomment">Hybrid Operators for Heterodimeric Repressor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Hybrid Operators for Heterodimeric Repressor==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Assumed that R4 is the highly specific repressor protein that well binds to O44, and so does R2 to O22, well, then it is clear that asymmetric O24 can be bound to both by R2 and R4. However, neither of them is very suitable because R4 is highly specific only for O44 rather than O22, and vice versa. Luckily these LacI-family Repressors are also symmetric, each of them is combined by two same monomers, so one side of R2 can bind to O24's left-half strand while the other side would not bring about any remarkable effect on the right. To bind to half strand could still maintain repression, but distinctly it could no longer be that strong. Now we can say that hybrid operator O24 is a “weaker” operator for R2 or R4 because their weaker binding compared with R2+O22 or R4+O44. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Furthermore, the dimerization region of our artificial repressors are <del class="diffchange diffchange-inline">same</del>, so they can combine into the heterodimeric form, for example, heterodimer R2.<del class="diffchange diffchange-inline">R4, </del>one side of R2 can bind to O24's left-half strand while the other side of R4 bind on the right half as well.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Furthermore, the dimerization region of our artificial repressors are <ins class="diffchange diffchange-inline">uniform</ins>, so they can combine into the heterodimeric form, for example, heterodimer R2<ins class="diffchange diffchange-inline">~R4</ins>. <ins class="diffchange diffchange-inline">Its </ins>one side of R2 can bind to O24's left-half strand <ins class="diffchange diffchange-inline">specifically </ins>while the other side of R4 bind on the right half as well.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:USTC_HybridOperator.png|500px|thumb|center|Supposed mechanism of hybrid operator. It appears as a weak operator to homodimers, and only heterodimers can bind to it tightly.]]</div></td></tr>
</table>MaRui