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Revision as of 16:52, 21 October 2007

Cis-acting Bio-Logic Gates

Model

Schemes of Bio-Logic Promoters

Repression Assay

Build Up Promoter Family

Firstly, we extend both sides of the conservative region for transcriptional initiation of PlacUV5[], including -35 box，-10 box and +1 starting point, with two non-sense sequence selected from random groups. The product is named as P_template1 as it is the template for the promoter family. These two non-sense sequence have three main characters:

1. They will never include the restriction enzyme cutting sites that will be involved in the whole study;
2. They will never include the recognition sites of RNA Polymerases and those of either of the two repressors;
3. They will never present in complicated structures.

Secondly, another group of primers, of which the elongation region at 5’ end may contain a unique operator sequence or each, is applied at both ends of P_template1, equipping us with an according group of promoters with complete structures. These promoters can include variant operator sequences at different position in flank of the conservative region.

Then the promoter fragments are digested and loaded into repression-reporter plasmid, with two

Solo-Repression Assay

Figure Solo-Repression

Co-Repression Assay

Figure Co-Repression

Final Results

Best NAND

Best NOR

File:USTC NestNOT.png Best NOT

References