USTC/OperatorPosition

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  Operator positioning plays a role in determining in vivo level of repression.[[USTC/OperatorPosition#References|[1]]]Both operators with strong and weak repression are required to the gates . So we tested the positioning effects systematically.
[[Image:USTC_RelativePromoterActivity.png|thumb|600px|center|'''Figure 1''' Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence.  
[[Image:USTC_RelativePromoterActivity.png|thumb|600px|center|'''Figure 1''' Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence.  
Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.
Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.
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[[Image:USTC_EffectOfPosition.png|thumb|600px|center|'''Figure 2''' Assumed influence on solo-repression when an operator is at a different position.]]
[[Image:USTC_EffectOfPosition.png|thumb|600px|center|'''Figure 2''' Assumed influence on solo-repression when an operator is at a different position.]]
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== References ==
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1. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', <i>Genes Dev</i> 3(2), 185--197.

Revision as of 06:08, 26 October 2007

  Operator positioning plays a role in determining in vivo level of repression.[1]Both operators with strong and weak repression are required to the gates . So we tested the positioning effects systematically.
Figure 1 Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence. Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.
Figure 2 Assumed influence on solo-repression when an operator is at a different position.

References

1. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', Genes Dev 3(2), 185--197.