USTC/OperatorPosition

From 2007.igem.org

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Operator positioning plays a role in determining in vivo level of repression[[USTC/OperatorPosition#References|[1]]].Both operators with strong and weak repression are required to the gates . So we tested the positioning effects systematically.
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Different locations of an operator plays a role in determining the intensity of repression in vivo[[USTC/OperatorPosition#References|[1]]].Both operators with strong and weak repression are required to the gates. Therefore, we systematically tested the influence of location factor on the promoter activity.
[[Image:USTC_RelativePromoterActivity.png|thumb|600px|center|'''Figure 1''' Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence.  
[[Image:USTC_RelativePromoterActivity.png|thumb|600px|center|'''Figure 1''' Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence.  
Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.
Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.

Revision as of 12:27, 26 October 2007

Different locations of an operator plays a role in determining the intensity of repression in vivo[1].Both operators with strong and weak repression are required to the gates. Therefore, we systematically tested the influence of location factor on the promoter activity.

Figure 1 Left: the influence on the promoter’s activity when a single operator is at a different position upstream of the consensus sequence. Right: the influence on the promoter’s activity when a single operator is at a different position downstream of the consensus sequence.
Figure 2 Assumed influence on solo-repression when an operator is at a different position.

References

1. Elledge, S. J. & Davis, R. W. (1989), 'Position and density effects on repression by stationary and mobile DNA-binding proteins.', Genes Dev 3(2), 185--197.