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- | ==The Model==
| + | Hey buddy found out you little secret, you've had two jobs over summer: |
- | This section describes a preliminary model for...
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- | ===System network topology===
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- | This section outlines a basic model for our system, Infector Detector. We choose to condition the system in a manner holds for both constructs. Essentially construct 2 only differs with respect to absence of pTET constitutive promoter, and where LuxR is introduced into the system in purified form - in other words - LuxR is controllable for construct 2.
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- | Figure 2 and 3 illustrate the system, achieved through construct 1 and 2 respectively. Table 1 exhibits the network of chemical reactions which govern its behaviour.
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- | ==Formulation of the problem==
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- | As described earlier, catheter-associated urinary tract infection (CAUTI) in the clinical setting is a prevalent problem with extensive economic impact. The underlying cause of many such infections can be attributed to the formation of biofilm, by aggregating-bacteria on the surface of urinary catheters.
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- | [[Image: IC07_QS.png|right|thumb|500px| Role of AHL (HSL) quorum-sensing in biofilm formation]]
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- | Infector Detector (ID) is a simple biological detector, which serves to expose bacterial biofilm. It functions by exploiting the inherent AHL (Acetyl Homoserine Lactone) production employed by certain types of quorum-sensing bacteria, in the formation of such structures.<br>
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- | Our project attempts to improve where previous methods of biofilm detection have proven ineffective: first and foremost, by focussing on the sensitivity of the system, to markers of biofilm: in this case, low levels of AHL production (which represents the bacterial "chatter" of such aggregating bacteria).
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- | In doing so, a complete investigation of the level of sensitivity to AHL concentration needs to be performed - in other words, what is the minimal AHL concentration for appreciable expression of a chosen reporter protein. Furthermore, establish a functional range for possible AHL detection. How does increased AHL concentration impact on the maximal output of reporter protein?<br>
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- | Finally, how can the system performance be tailored, by exploiting possible state variables (e.g. varying initial LuxR concentration and/or concentration of pLux promoters).
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- | The system performance here revolves most importantly around AHL sensitivity; however, the effect on the maximal output of fluorescent reporter protein and response time is, likewise, of great importance.
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- | ==Approach==
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- | A deterministic, continuous approximation is considered.
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- | ==[https://2007.igem.org/User:Jjk105/Simulations Simulations]== | + | |