Vaibhavi Umesh Notebook
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==[[User:Vaibhaviu|Vaibhaviu]] 19:02, 1 June 2007 (EDT)== | ==[[User:Vaibhaviu|Vaibhaviu]] 19:02, 1 June 2007 (EDT)== | ||
Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:<br> | Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:<br> | ||
| - | [[Image:Berk-VU060107gel1.jpg|100px]] | + | [[Image:Berk-VU060107gel1.jpg|100px|left]] |
| - | It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file: | + | It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file: |
| - | [[Image:Berk-VU060107gel2.jpg|100px|right]] | + | [[Image:Berk-VU060107gel2.jpg|100px|right]] |
We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today. | We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today. | ||
Revision as of 23:17, 1 June 2007
Vaibhaviu 19:02, 1 June 2007 (EDT)
Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:
It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file:
We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today.
