Vaibhavi Umesh Notebook

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My Construction Files
My Sequencing Files


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notes

to do

Vaibhaviu 19:02, 1 June 2007 (EDT)

Today we cloned an RFP basic part into pBca9145. So, we did the construction file for pBca9145-Bca1145. We did a PCR of the RFP gene according to the construction file, and the gel is here:

Berk-VU060107gel1.jpg

It's a little dilute, but it should work nonetheless. We also digested the vector according to the construction file:

Berk-VU060107gel2.jpg

We gel purified the larger of the two bands. It looks like the digest went pretty well, as we see no single-cut plasmid DNA. We took it all the way through to transformation into DH10B cells today.