Valencia/Ligation

From 2007.igem.org

Information obtained from:

Promega

We used one ligase for the ligations:

  • T4 DNA Ligase

Protocol obtained from: [http://www.promega.com/tbs/9pim180/9pim180.pdf Promega]


Ligation

Usually we ligate following the recommendations from the commercial information:

VLupa.jpg we have had good results with a molar ratio of 3:1

  • Ligase
total DNA 20-200 ng
enzyme 1 uL
2X Rapid Ligation buffer 10 uL
ddwater up to 20 uL (sometimes, if the samples are in low concentrations, we go up to 40uL)