Vector choice
From 2007.igem.org
| Line 32: | Line 32: | ||
HindIII 233 | HindIII 233 | ||
| - | PstI 249 | + | PstI 249 |
| - | SalI 251 | + | |
| - | XbaI 257 | + | SalI 251 |
| - | BamHI | + | |
| - | SmaI 270 | + | XbaI 257 |
| - | KpnI 276 | + | |
| - | SacI 282 | + | BamHI 263 |
| - | EcoRI | + | |
| - | NarI 446 | + | SmaI 270 |
| - | SpeI 550 | + | |
| - | NheI 1801 | + | KpnI 276 |
| - | BglII | + | |
| - | EcoRV | + | SacI 282 |
| - | PmlI 2780 | + | |
| - | AatII | + | EcoRI 284 |
| + | |||
| + | NarI 446 | ||
| + | |||
| + | SpeI 550 | ||
| + | |||
| + | NheI 1801 | ||
| + | |||
| + | BglII 1986 | ||
| + | |||
| + | EcoRV 2455 | ||
| + | |||
| + | PmlI 2780 | ||
| + | |||
| + | AatII 2921 | ||
Revision as of 19:52, 10 September 2007
Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb.
Selected features
lac_promoter 142 - 171
M13_pUC_rev_primer 185 - 207
M13_reverse_primer 206 - 224
M13_forward20_primer 306 - 290
M13_pUC_fwd_primer 321 - 299
CEN4 504 - 1356
Orf frame 2 2740 - 2066
TRP1 2740 - 2066
AmpR_promoter 2983 - 3011
Orf frame 2 3053 - 3913
Ampicillin 3053 - 3913
pBR322_origin 4068 - 4687
Unique restriction sites
HindIII 233
PstI 249
SalI 251
XbaI 257
BamHI 263
SmaI 270
KpnI 276
SacI 282
EcoRI 284
NarI 446
SpeI 550
NheI 1801
BglII 1986
EcoRV 2455
PmlI 2780
AatII 2921
