http://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&feed=atom&action=historyVincent Parker Notebook - Revision history2024-03-29T15:36:34ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=39745&oldid=prevVparker at 06:28, 26 October 20072007-10-26T06:28:43Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 06:28, 26 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Put in 5 different flasks of BLR mixtures.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Put in 5 different flasks of BLR mixtures.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Time put into incubator 10:45 OD @ 11:45 OD @ 12:20 <del class="diffchange diffchange-inline"> </del>OD @ 12:50 OD @ 1:05 OD @ 1:25</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Time put into incubator 10:45 OD @ 11:45 OD @ 12:20 <ins class="diffchange diffchange-inline"> </ins>OD @ 12:50 OD @ 1:05 OD @ 1:25</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1.BLR 1).096 1).17 <del class="diffchange diffchange-inline"> </del>1).32 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1.BLR 1).096 1).17 <ins class="diffchange diffchange-inline"> </ins>1).32 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2.BLR+Plasmid 2).4 2).08 <del class="diffchange diffchange-inline"> </del>2).126 2).17 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>2.BLR+Plasmid 2).4 2).08 <ins class="diffchange diffchange-inline"> </ins>2).126 2).17 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3.BLR+BA Plasmid 3).7 3).11 <del class="diffchange diffchange-inline"> </del>3).2 3).27 3).255 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>3.BLR+BA Plasmid 3).7 3).11 <ins class="diffchange diffchange-inline"> </ins>3).2 3).27 3).255 </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>4.BLR+PLasmid+Inducer 4).056 4).04 <del class="diffchange diffchange-inline"> </del>4).04 4).032</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>4.BLR+PLasmid+Inducer 4).056 4).04 <ins class="diffchange diffchange-inline"> </ins>4).04 4).032</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>5.BLR+BA Plasmid+Inducer 5).056 5).04 <del class="diffchange diffchange-inline"> </del>5).04 5).039</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>5.BLR+BA Plasmid+Inducer 5).056 5).04 <ins class="diffchange diffchange-inline"> </ins>5).04 5).039</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Seeing as growth stopped after a certain amount of time it was decided to redo the assay.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Seeing as growth stopped after a certain amount of time it was decided to redo the assay.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Did however place a mol in the lyopholizer overnight to test resistance to freeze-drying.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Did however place a mol in the lyopholizer overnight to test resistance to freeze-drying.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 01:49, <del class="diffchange diffchange-inline">26 October </del>2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 01:49, <ins class="diffchange diffchange-inline">3 August </ins>2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Resuspend tubes taken out of lyopholizer in dilutions of X, 10X, 100X, and 1000X.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Resuspended in 2 mols of LB broth. Vortexed out to mix.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*once resuspended transformed onto plates and left in incubator to grow overnight.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 6 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*From plates left in incubator only colonies grew on X and 10X plates of the BLR+BA Plasmid and BLR+Plasmid(TBRAT plasmid)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Decided to retry experiment with a new variable: sucrose.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Took .4 grams and dissolved into 10 mil of water. Then added to shaker flasks of BLR, BLR+BA Plasmid, and BLR+BA Plasmid+Inducer. Used only BA plasmid because produced best results.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Placed in Incubator @ 1:45 OD @ 2:45 OD @ 3:45 OD @ 4:15</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">1.BLR 1).156 1).137 1).150 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">2.BLR+BA Plasmid 2).068 2).07 2).69 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">3.BLR+BA Plasmid+Inducer 3).065 3).065 3).62 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">4.(S)BLR 4).142 4).152 4).168 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">5.(S)BLR+BA Plasmid 5).061 5).063 5).062 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">6.(S)BLR+BA Plasmid+inducer 6).064 6).063 6).059 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*In order to continue experimentation I needed to streak new BLR and put the BA Operon into liquid media to use for tomorrow.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 7 August 2007 (EDT) </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Assay seemed to be missing something. I decided to reproduce the experiment but with new media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* grew new media and ran procedures for lypholization.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 8 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Resuspended 1 mil of each flask and resuspended for transformation.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*6 plated in AMP(BLR+plasmids) 2 plated in agar(BLR only)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 9 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*No living colonies seen on any plate.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 10 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Since i need to switch mid log media i will have to use Ptet strain as my new vector.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Need to ligate otsBA-TT to Ptet vector.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Did ligation then second digestion with Bam/Bgl.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 12 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Came in this weekend to miniprep real fast and i took 5 colonies of Nhu's plates and put them into liquid media and then into the incibator.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 01:49, 13-17 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">After having successflly cloned my operons into Ptet strain. I realized that I have run out of time on my project over the summer. My conclusion is that indeed trehalose does help with dessication in biological organisms and that from my experiment my operon produced better from not having an inducer and plated at higher concentration rates. If my part of the project were continued I could have continued experimenting with the Ptet strain and eventually found a promoter and RBS site that would produce better results that would have more percentage the bacto-blood recover after having been freeze dried. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> It was a great experience for me over the summer and it was great working with all of the iGEM crew.</ins></div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=39680&oldid=prevVparker at 05:49, 26 October 20072007-10-26T05:49:46Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 05:49, 26 October 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Resuspended cells that were freeze dried and made a dilution which was then transformed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Resuspended cells that were freeze dried and made a dilution which was then transformed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Suspended some mid-log tubes in drierite.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Suspended some mid-log tubes in drierite.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Checked plates for growth of TBRAT.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Put in 5 different flasks of BLR mixtures.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Time put into incubator 10:45 OD @ 11:45 OD @ 12:20 OD @ 12:50 OD @ 1:05 OD @ 1:25</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1.BLR 1).096 1).17 1).32 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2.BLR+Plasmid 2).4 2).08 2).126 2).17 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3.BLR+BA Plasmid 3).7 3).11 3).2 3).27 3).255 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">4.BLR+PLasmid+Inducer 4).056 4).04 4).04 4).032</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">5.BLR+BA Plasmid+Inducer 5).056 5).04 5).04 5).039</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Seeing as growth stopped after a certain amount of time it was decided to redo the assay.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Did however place a mol in the lyopholizer overnight to test resistance to freeze-drying.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 01:49, 26 October 2007 (EDT)</ins></div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=12848&oldid=prevVparker at 18:10, 2 August 20072007-08-02T18:10:23Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:10, 2 August 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Could not transform since we were lacking of Righty competent cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Could not transform since we were lacking of Righty competent cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped otsB-RBS-otsA-TT(BRAT).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped otsB-RBS-otsA-TT(BRAT).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Used wrong enzyme to digest T7-RBS and BRAT. Fact thatT7-RBS was too small to gel extract did not occur to me. Re-set up a digestion using Bsa1 and BamHI. Sizes of bands would be even so i also digested T7-RBS with <del class="diffchange diffchange-inline">Alwn1</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Used wrong enzyme to digest T7-RBS and BRAT. Fact thatT7-RBS was too small to gel extract did not occur to me. Re-set up a digestion using Bsa1 and BamHI. Sizes of bands would be even so i also digested T7-RBS with <ins class="diffchange diffchange-inline">AlwnI</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digested BRAT with Bsa1 and BglII.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digested BRAT with Bsa1 and BglII.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sent off sequences for BRAT.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sent off sequences for BRAT.</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 157:</td>
<td colspan="2" class="diff-lineno">Line 157:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Just transferred TBRAT into liquid media. (The addition of T7-RBS is too small to make a significant difference in band sizes.)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Just transferred TBRAT into liquid media. (The addition of T7-RBS is too small to make a significant difference in band sizes.)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <del class="diffchange diffchange-inline">2 August </del>2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <ins class="diffchange diffchange-inline">27 July </ins>2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <del class="diffchange diffchange-inline">2 August </del>2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Digested otsBA-TT with using BsaI, BglII.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <del class="diffchange diffchange-inline">2 August </del>2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Ligated digestion of otsBA-TT with T7-RBS.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <del class="diffchange diffchange-inline">2 August </del>2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*After heat kill digested again with BglII/BamHI.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <del class="diffchange diffchange-inline">2 </del>August 2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Transformed BA-Operon to righty cells.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Miniprepped TBRAT and sent off 28 sample sequences of TBRAT and BA-Operon.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Transformed TBRAT into BLR plasmid.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <ins class="diffchange diffchange-inline">28 July </ins>2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Placed colonies from TBRAT and BA-Operon into liquid media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <ins class="diffchange diffchange-inline">30 July </ins>2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Miniprepped BA-Operon and then transformed into the BLR strain as well.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Made variable samples containing BLR, BLR+TBRAT, BLR+TBRAT+inducer.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Streaked BLR onto a plate.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Also transformed more T7-RBS running low on supplies.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <ins class="diffchange diffchange-inline">31 July </ins>2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Took out tubes and checked plates. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Transferred BA-Operon and T7-RBS into liquid media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Learned about mid-log and then placed media into incubator to reach mid-log.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Used Specto to check O.D. of the media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Used liquid nitrogen and froze pelleted samples once mid-log was reached and then went upstairs and put into lypholizer overnight.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, <ins class="diffchange diffchange-inline">1 </ins>August 2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Put BA-Operon into incubator to reach mid-log like with TBRAT.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Resuspended cells that were freeze dried and made a dilution which was then transformed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Suspended some mid-log tubes in drierite.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=12847&oldid=prevVparker at 17:53, 2 August 20072007-08-02T17:53:41Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 17:53, 2 August 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 156:</td>
<td colspan="2" class="diff-lineno">Line 156:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Colony PCRed otsBA and transferred to liquid media.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Colony PCRed otsBA and transferred to liquid media.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Just transferred TBRAT into liquid media. (The addition of T7-RBS is too small to make a significant difference in band sizes.)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Just transferred TBRAT into liquid media. (The addition of T7-RBS is too small to make a significant difference in band sizes.)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 13:53, 2 August 2007 (EDT)</ins></div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=11656&oldid=prevVparker at 18:53, 26 July 20072007-07-26T18:53:20Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:53, 26 July 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 142:</td>
<td colspan="2" class="diff-lineno">Line 142:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Did not heat kill. Set back one hour to heat kill and re-digest with BamHI and BglII.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Did not heat kill. Set back one hour to heat kill and re-digest with BamHI and BglII.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Could not transform since we were lacking of Righty competent cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Could not transform since we were lacking of Righty competent cells.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 14:50, 26 July 2007 (EDT)</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped otsB-RBS-otsA-TT(BRAT).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped otsB-RBS-otsA-TT(BRAT).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Used wrong enzyme to digest T7-RBS and BRAT. Fact thatT7-RBS was too small to gel extract did not occur to me. Re-set up a digestion using Bsa1 and BamHI. Sizes of bands would be even so i also digested T7-RBS with Alwn1.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Used wrong enzyme to digest T7-RBS and BRAT. Fact thatT7-RBS was too small to gel extract did not occur to me. Re-set up a digestion using Bsa1 and BamHI. Sizes of bands would be even so i also digested T7-RBS with Alwn1.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digested BRAT with Bsa1 and BglII.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digested BRAT with Bsa1 and BglII.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">sent </del>off sequences for BRAT.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">Sent </ins>off sequences for BRAT.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:50, <del class="diffchange diffchange-inline">26 </del>July 2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:50, <ins class="diffchange diffchange-inline">25 </ins>July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*BRAT sequences came in all looking good but decided to use 2S2 as clone Saver.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*BRAT sequences came in all looking good but decided to use 2S2 as clone Saver.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Gel extracted overnight digestiong of T7-RBS and BRAT.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Gel extracted overnight digestiong of T7-RBS and BRAT.</div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=11655&oldid=prevVparker at 18:50, 26 July 20072007-07-26T18:50:49Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:50, 26 July 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 135:</td>
<td colspan="2" class="diff-lineno">Line 135:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion of otsBA, gel extracted and ligated to TT.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion of otsBA, gel extracted and ligated to TT.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Out of Righty comp. cells so put in freezer until more are available.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Out of Righty comp. cells so put in freezer until more are available.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Miniprepped otsB-RBS-otsA-TT.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Checked sequences from Richard and otsA-TT and otsBA are both good samples.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Checked sequences from Richard and otsA-TT and otsBA are both good samples.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Miniprepped otsBA quickchange.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Took risk and digested otsBA and then ligated the TT terminator.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Did not heat kill. Set back one hour to heat kill and re-digest with BamHI and BglII.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Could not transform since we were lacking of Righty competent cells.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 14:50, 26 July 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Miniprepped otsB-RBS-otsA-TT(BRAT).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Used wrong enzyme to digest T7-RBS and BRAT. Fact thatT7-RBS was too small to gel extract did not occur to me. Re-set up a digestion using Bsa1 and BamHI. Sizes of bands would be even so i also digested T7-RBS with Alwn1.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Digested BRAT with Bsa1 and BglII.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*sent off sequences for BRAT.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 14:50, 26 July 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*BRAT sequences came in all looking good but decided to use 2S2 as clone Saver.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Gel extracted overnight digestiong of T7-RBS and BRAT.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Ligated together to make TBRAT. Transformed 4 TBRATS into regular comp cells and two otsBA-TT's into righty comp. cells.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[User:Vparker|Vparker]] 14:50, 26 July 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Colonies on all plates.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Colony PCRed otsBA and transferred to liquid media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Just transferred TBRAT into liquid media. (The addition of T7-RBS is too small to make a significant difference in band sizes.)</ins></div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=11201&oldid=prevVparker at 18:24, 24 July 20072007-07-24T18:24:22Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:24, 24 July 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 124:</td>
<td colspan="2" class="diff-lineno">Line 124:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 21 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 21 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped samples for Nhu and my otsA-TT's.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped samples for Nhu and my otsA-TT's.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Colony PCRed the quickchange <del class="diffchange diffchange-inline">og </del>otsBA and simultaneously transferred to liquid media.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Colony PCRed the quickchange <ins class="diffchange diffchange-inline">of </ins>otsBA and simultaneously transferred to liquid media.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">Digested otsB-RBS and otsA-TT with Eco/Bam and Eco/Bgl.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Gel extraction and purification then ligated together.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Another digested of Bgl/Bam and then transformed into 4 dishes. S1, S2, L3, & L2. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 23 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 23 July 2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Miniprepped otsBA quickchange.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Sent of 16 sequencing samples of otsA-TT and otsBA.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Colony PCR of otsB-RBS-otsA-TT proved positive.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Digestion of otsBA, gel extracted and ligated to TT.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Out of Righty comp. cells so put in freezer until more are available.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Miniprepped otsB-RBS-otsA-TT.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Checked sequences from Richard and otsA-TT and otsBA are both good samples.</ins></div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=11200&oldid=prevVparker at 18:17, 24 July 20072007-07-24T18:17:30Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:17, 24 July 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 93:</td>
<td colspan="2" class="diff-lineno">Line 93:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Left PCR of otsB. Gel extracted, Cleaned up, and digested.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Left PCR of otsB. Gel extracted, Cleaned up, and digested.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Pcr 5-7/6-4 </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Pcr 5-7/6-4 </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 16 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 16 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligation of L otsA and R TT.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligation of L otsA and R TT.</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 98:</td>
<td colspan="2" class="diff-lineno">Line 99:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Gelled 5-7/6-4 PCR and digested with BglII/XhoI and then ligated to clone vector.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Gelled 5-7/6-4 PCR and digested with BglII/XhoI and then ligated to clone vector.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*otsBA Transformed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*otsBA Transformed.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 17 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 17 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Did not show up. Bonds did ligation of otsA-TT again because plates did not grow.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Did not show up. Bonds did ligation of otsA-TT again because plates did not grow.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Colony PCRed otsBA and all samples worked well.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Colony PCRed otsBA and all samples worked well.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 18 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 18 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped otsBA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprepped otsBA.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sent off 2 samples for sequencing. Went to A's Game.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sent off 2 samples for sequencing. Went to A's Game.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digested L otsBA, L otsA and R TT.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digested L otsBA, L otsA and R TT.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 19 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:52, 19 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligated and digested otsA-TT. Will tranform.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ligated and digested otsA-TT. Will tranform.</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 110:</td>
<td colspan="2" class="diff-lineno">Line 114:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ordered oligos to quickchange out the mutation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ordered oligos to quickchange out the mutation.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Caught up on construction files for composite parts.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Caught up on construction files for composite parts.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 20 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 20 July 2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Colony PCRed otsA-TT with one being a big ligation and one bing a small ligation. They were thus dubbed L and S.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Transferred to liquid media immediately after colony PCR.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Prepared the oligos for the quickchange of otsBA to PCR out the mutation.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Then did quickchange of otsBA, then digested and tranformed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Clone saved otsB-RBS.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 21 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 21 July 2007 (EDT)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Miniprepped samples for Nhu and my otsA-TT's.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Colony PCRed the quickchange og otsBA and simultaneously transferred to liquid media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 23 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 23 July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=11191&oldid=prevVparker at 18:08, 24 July 20072007-07-24T18:08:47Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:08, 24 July 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 110:</td>
<td colspan="2" class="diff-lineno">Line 110:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ordered oligos to quickchange out the mutation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ordered oligos to quickchange out the mutation.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Caught up on construction files for composite parts.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Caught up on construction files for composite parts.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, <del class="diffchange diffchange-inline">24 </del>July 2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, <ins class="diffchange diffchange-inline">20 </ins>July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, <del class="diffchange diffchange-inline">24 </del>July 2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, <ins class="diffchange diffchange-inline">21 </ins>July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, <del class="diffchange diffchange-inline">24 </del>July 2007 (EDT)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, <ins class="diffchange diffchange-inline">23 </ins>July 2007 (EDT)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</div></td></tr>
</table>Vparkerhttp://2007.igem.org/wiki/index.php?title=Vincent_Parker_Notebook&diff=11190&oldid=prevVparker at 18:08, 24 July 20072007-07-24T18:08:23Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:08, 24 July 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 109:</td>
<td colspan="2" class="diff-lineno">Line 109:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sequences for otsBA came in and mutation still there.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Sequences for otsBA came in and mutation still there.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ordered oligos to quickchange out the mutation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Ordered oligos to quickchange out the mutation.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Caught up on construction files for composite parts.[</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Caught up on construction files for composite parts.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[<ins class="diffchange diffchange-inline">[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[User:Vparker|Vparker]] 14:08, 24 July 2007 (EDT)</ins></div></td></tr>
</table>Vparker