Week 10

From 2007.igem.org

(Difference between revisions)
Line 55: Line 55:
-R0051+I763020(I763011);
-R0051+I763020(I763011);
-
-I763009+S0100(I763023);
+
-I763009+P0412(I763021);
-I763009+S03520(I763022);
-I763009+S03520(I763022);
 +
 +
*Digestion for:
 +
 +
-I763019 with Eco/Xba;
 +
 +
-I763026 with Eco/Spe;
 +
 +
-I763030 with Eco/Spe;
 +
 +
-I763011 with Xba/Pst1;
 +
 +
-R0051+I13507(I763007) with Xba/Pst1;
 +
 +
-I763021 with Eco/Spe;
 +
 +
*Control digestion for I763019 with Eco/Pst1;
 +
*Band extraction for
 +
 +
-I763019;
 +
 +
-I763026;
 +
 +
-I763011;
 +
 +
-R0051+I13507(I763007);
 +
 +
*New control digestion for:
 +
 +
-I763019 with Eco/Xba;
 +
 +
-I763021 with Eco/Spe;
 +
 +
-I763026 with Eco/Spe;
 +
 +
-I763030 with Eco/Spe;

Revision as of 20:00, 7 September 2007

09/03/07
  • Miniprep for:

-R0040+S0100;

-R0040+S03520;

-I763005+I763015;

-J52034+E0022;

  • Digestion for:

-R0040+S0100 with Eco/Spe;

-R0040+S03520 with Eco/Spe;

-I763005+I763015 with Spe/Pst1;

-J52034+E0022with Spe/Pst1;

-B0015 with Eco/Xba;

  • Band exstraction;
  • Ligation for:

-I763025+B0015(I763026);

-I763024+B0015(I763030);

-I763016+I763020(I763019);

-I763009+P0412(I763021);

-I763009+S0100(I763023);

-I763009+S03520(I763022);

-R0051+I763020(I763011);


09/04/07
  • There are colonies for every ligations.


09/05/07

Miniprep for:

-I763016+I763020(I763019);

-I763025+B0015(I763026);

-I763024+B0015(I763030);

-R0051+I763020(I763011);

-I763009+P0412(I763021);

-I763009+S03520(I763022);

  • Digestion for:

-I763019 with Eco/Xba;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;

-I763011 with Xba/Pst1;

-R0051+I13507(I763007) with Xba/Pst1;

-I763021 with Eco/Spe;

  • Control digestion for I763019 with Eco/Pst1;
  • Band extraction for

-I763019;

-I763026;

-I763011;

-R0051+I13507(I763007);

  • New control digestion for:

-I763019 with Eco/Xba;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;


09/06/07


09/07/07

Back