Week 10
From 2007.igem.org
(Difference between revisions)
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-[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba; | -[http://partsregistry.org/Part:BBa_B0015 B0015] with Eco/Xba; | ||
- | *Band extraction; | + | *Band extraction from gel for all digestion; |
*Ligation for: | *Ligation for: | ||
Line 42: | Line 42: | ||
*There are colonies for every ligations. | *There are colonies for every ligations. | ||
*Meeting. | *Meeting. | ||
+ | |||
::'''09/05/07''' | ::'''09/05/07''' | ||
Line 73: | Line 74: | ||
*Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1; | *Control digestion for [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Pst1; | ||
- | *Band extraction for | + | *Band extraction from gel for: |
- [http://partsregistry.org/Part:BBa_I763019 I763019]; | - [http://partsregistry.org/Part:BBa_I763019 I763019]; | ||
Line 85: | Line 86: | ||
*New control digestion for: | *New control digestion for: | ||
- | -[ http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe; | + | -[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe; |
- | - [http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1; | + | -[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Pst1; |
- | - [http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; |
- | - [http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe; |
- | - [http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763030 I763030] with Eco/Spe; |
*Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026]; | *Band extraction for [http://partsregistry.org/Part:BBa_I763019 I763019] (digested with Xba/Spe and with Xba/Pst1), [http://partsregistry.org/Part:BBa_I763026 I763026]; | ||
*Ligation O/N for: | *Ligation O/N for: | ||
- | - [http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019](http://partsregistry.org/Part:BBa_I763028 I763028]); | + | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763019 I763019]([http://partsregistry.org/Part:BBa_I763028 I763028]); |
- | - [http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]); | + | -[http://partsregistry.org/Part:BBa_I763026 I763026]+ [http://partsregistry.org/Part:BBa_I763004 I763004]([http://partsregistry.org/Part:BBa_I763027 I763027]); |
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Digestion for: | Digestion for: | ||
- | - [http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba; | + | -[http://partsregistry.org/Part:BBa_I763019 I763019] with Eco/Xba; |
- | -[ http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; | + | -[http://partsregistry.org/Part:BBa_I763021 I763021] with Eco/Spe; |
- | -[ http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe. | + | -[http://partsregistry.org/Part:BBa_I763026 I763026] with Eco/Spe. |
*Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026]. | *Band extraction from electrophoresis gel for [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763026 I763026]. | ||
*Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts. | *Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts. | ||
*Transformations for the new ligations. | *Transformations for the new ligations. | ||
+ | |||
+ | |||
::'''09/07/07''' | ::'''09/07/07''' | ||
+ | *Glycerol stocks preparation; | ||
+ | *We check without plasmid [[bacteria]] [[Growth Curve - without plasmid bacteria | growth curve]] to individuate a model parameter. | ||
+ | |||
- | [[Bologna|Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:53, 26 October 2007
- 09/03/07
- Miniprep for:
- Digestion for:
-I763005+I763015 with Spe/Pst1;
-B0015 with Eco/Xba;
- Band extraction from gel for all digestion;
- Ligation for:
- 09/04/07
- There are colonies for every ligations.
- Meeting.
- 09/05/07
Miniprep for:
- Digestion for:
- I763019 with Eco/Xba;
- I763026 with Eco/Spe;
- I763030 with Eco/Spe;
- I763011 with Xba/Pst1;
- R0051+ I13507(I763007) with Xba/Pst1;
- I763021 with Eco/Spe;
- Control digestion for I763019 with Eco/Pst1;
- Band extraction from gel for:
- I763019;
- I763026;
- I763011;
- New control digestion for:
-I763019 with Xba/Spe;
-I763019 with Xba/Pst1;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe;
-I763030 with Eco/Spe;
- 09/06/07
Digestion for:
-I763019 with Eco/Xba;
-I763021 with Eco/Spe;
-I763026 with Eco/Spe.
- Band extraction from electrophoresis gel for I763019 and I763026.
- Ligation for I763027 with new extracts.
- Transformations for the new ligations.
- 09/07/07
- Glycerol stocks preparation;
- We check without plasmid bacteria growth curve to individuate a model parameter.