Week 10

From 2007.igem.org

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*Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts.
*Ligation for [http://partsregistry.org/Part:BBa_I763027 I763027] with new extracts.
*Transformations for the new ligations.
*Transformations for the new ligations.
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 +
::'''09/07/07'''
::'''09/07/07'''
*Glycerol stocks preparation;
*Glycerol stocks preparation;
-
*We check without plasmid [[bacteria]] growth curve to individuate a model parameter.
+
*We check without plasmid [[bacteria]] [[Growth Curve - without plasmid bacteria | growth curve]] to individuate a model parameter.
-
[[Bologna|Back]]
+
[[Bologna#Diary | Back]]

Latest revision as of 15:53, 26 October 2007

09/03/07
  • Miniprep for:

-R0040+S0100;

-R0040+S03520;

-I763005+I763015;

-J52034+E0022;

  • Digestion for:

-R0040+S0100 with Eco/Spe;

-R0040+S03520 with Eco/Spe;

-I763005+I763015 with Spe/Pst1;

-J52034+E0022 with Spe/Pst1;

-B0015 with Eco/Xba;

  • Band extraction from gel for all digestion;
  • Ligation for:

-I763025+B0015(I763026);

-I763024 +B0015(I763030);

-I763016+I763020(I763019);

-I763009+P0412(I763021);

-I763009+S0100(I763023);

-I763009+S03520(I763022);

-R0051+I763020(I763011);


09/04/07
  • There are colonies for every ligations.
  • Meeting.


09/05/07

Miniprep for:

- I763016+ I763020 (I763019);

- I763025+ B0015 (I763026);

- I763024+ B0015 (I763030);

- R0051+ I763020 (I763011);

- I763009+ P0412 (I763021);

- I763009+ S03520(I763022);

  • Digestion for:

- I763019 with Eco/Xba;

- I763026 with Eco/Spe;

- I763030 with Eco/Spe;

- I763011 with Xba/Pst1;

- R0051+ I13507(I763007) with Xba/Pst1;

- I763021 with Eco/Spe;

  • Control digestion for I763019 with Eco/Pst1;
  • Band extraction from gel for:

- I763019;

- I763026;

- I763011;

- R0051+ I13507 (I763007);

  • New control digestion for:

-I763019 with Xba/Spe;

-I763019 with Xba/Pst1;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe;

-I763030 with Eco/Spe;

  • Band extraction for I763019 (digested with Xba/Spe and with Xba/Pst1), I763026;
  • Ligation O/N for:

-I763026+ I763019(I763028);

-I763026+ I763004(I763027);


09/06/07

Digestion for:

-I763019 with Eco/Xba;

-I763021 with Eco/Spe;

-I763026 with Eco/Spe.

  • Band extraction from electrophoresis gel for I763019 and I763026.
  • Ligation for I763027 with new extracts.
  • Transformations for the new ligations.


09/07/07
  • Glycerol stocks preparation;
  • We check without plasmid bacteria growth curve to individuate a model parameter.


Back