From 2007.igem.org

Revision as of 10:08, 20 September 2007 (view source) Silvia.tamarri2 (Talk | contribs) ← Older edit		Revision as of 10:17, 20 September 2007 (view source) Silvia.tamarri2 (Talk | contribs) Newer edit →
Line 85:		Line 85:
	-I763028(midi).		-I763028(midi).
	*Miniprep for:		*Miniprep for:
-	-	+	-I763028;
		+
		+	-pLac-cI-GFP;
		+
		+	-I763026;
		+
		+	-I763020.
		+	*Digestion for:
		+	-I763028 with Eco/Xba;
		+
		+	-I763026 with Eco/Spa;
		+
		+	-S0100 with Spa/Pst1;
		+
		+	-S0100 with Eco/Spa;
		+
		+	-I763020 with Xba/Pst1;
		+
		+	-pLac-cI-GFP with Xba/Pst1.
		+	*Band extraction from gel for all digestion except for pLac-cI-GFP. [[(photos)]]
		+
		+
	[[Bologna | Back]]		[[Bologna | Back]]

---

Revision as of 10:17, 20 September 2007

09/10/07

• Miniprep for:

-I763027;

-I763028;

-I763019;

-I763021+P0412;

-I763021+S03520;

-I763021+S0100;

Digestion for:

-I763027 with Xba/Spe;

-I763028 with Spe/Pst1 and with Xba/Spe;

-I763019 with Xba/Spe;

-I763021+P0412 with Eco/Spe;

-I763021+S03520 with Eco/Spe;

-I763021+S0100 with Eco/Spe;

• Band extraction from gel for all digestion;
• We have problems with J52034 and with I763019.
• Ligations for:

-I763028 + I763007

-S0100 + J04431, because we want to understand if LacI operates well;

-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.

09/11/07

• Digestion for:

-I763020 with Xba/Pst1;

-J22101 with Xba/Pst1;

09/12/07

• Control digestion before fluorescence tests. (photos)
• Ligations for:

-I7633005(Spe/Pst1) + J04031(Xba/Pst1);

-S0100(Eco/Spe) + J04031(Eco/Xba);

-I763015 (Eco/Spa) + J04031(Eco/Xba).

09/13/07

• We have find any colonies for I763015 + J04031;
• Miniprep for:

- I7633005 + J04031;

-S0100 + J04031;

-I763028.

• Control digestion for I763028.
• M9 Medium and lactose stocks preparation.

09/14/07

• Glicerol stocks for:

-I763028;

-pLac-cI-GFP;

-I763020;

-I763026.

• Fluorescence test for:

-I763028;

-pLac-cI-GFP;

-I763028(midi).

• Miniprep for:

-I763028;

-pLac-cI-GFP;

-I763026;

-I763020.

• Digestion for:

-I763028 with Eco/Xba;

-I763026 with Eco/Spa;

-S0100 with Spa/Pst1;

-S0100 with Eco/Spa;

-I763020 with Xba/Pst1;

-pLac-cI-GFP with Xba/Pst1.

• Band extraction from gel for all digestion except for pLac-cI-GFP. (photos)

Back