Week 11

From 2007.igem.org

(Difference between revisions)
Line 85: Line 85:
-I763028(midi).
-I763028(midi).
*Miniprep for:
*Miniprep for:
-
-
+
-I763028;
 +
 
 +
-pLac-cI-GFP;
 +
 
 +
-I763026;
 +
 
 +
-I763020.
 +
*Digestion for:
 +
-I763028 with Eco/Xba;
 +
 
 +
-I763026 with Eco/Spa;
 +
 
 +
-S0100 with Spa/Pst1;
 +
 
 +
-S0100 with Eco/Spa;
 +
 
 +
-I763020 with Xba/Pst1;
 +
 
 +
-pLac-cI-GFP with Xba/Pst1.
 +
*Band extraction from gel for all digestion except for pLac-cI-GFP. [[(photos)]]
 +
 
 +
 
[[Bologna | Back]]
[[Bologna | Back]]

Revision as of 10:17, 20 September 2007

09/10/07
  • Miniprep for:

-[http://partsregistry.org/Part:BBa_I763027 I763027];

-[http://partsregistry.org/Part:BBa_I763028 I763028];

-[http://partsregistry.org/Part:BBa_I763019 I763019];

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_P0412 P0412];

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S03520 S03520];

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S0100 S0100];

Digestion for:

-[http://partsregistry.org/Part:BBa_I763027 I763027] with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763028 I763028] with Spe/Pst1 and with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763019 I763019] with Xba/Spe;

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_P0412 P0412] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S03520 S03520] with Eco/Spe;

-[http://partsregistry.org/Part:BBa_I763021 I763021]+[http://partsregistry.org/Part:BBa_S0100 S0100] with Eco/Spe;

  • Band extraction from gel for all digestion;
  • We have problems with [http://partsregistry.org/Part:BBa_J52034 J52034] and with [http://partsregistry.org/Part:BBa_I763019 I763019].
  • Ligations for:

-[http://partsregistry.org/Part:BBa_I763028 I763028] + [http://partsregistry.org/Part:BBa_I763007 I763007]

-[http://partsregistry.org/Part:BBa_S0100 S0100] + [http://partsregistry.org/Part:BBa_J04431 J04431], because we want to understand if LacI operates well;

-[http://partsregistry.org/Part:BBa_J06550 J06550] + [http://partsregistry.org/Part:BBa_J04631 J04631], with this ligation we want to understand if it operates well, because it doesn't leak.

09/11/07
  • Digestion for:

-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;

-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;


09/12/07
  • Control digestion before fluorescence tests. (photos)
  • Ligations for:

-I7633005(Spe/Pst1) + J04031(Xba/Pst1);

-S0100(Eco/Spe) + J04031(Eco/Xba);

-I763015 (Eco/Spa) + J04031(Eco/Xba).


09/13/07
  • We have find any colonies for I763015 + J04031;
  • Miniprep for:

- I7633005 + J04031;

-S0100 + J04031;

-I763028.

  • Control digestion for I763028.
  • M9 Medium and lactose stocks preparation.


09/14/07
  • Glicerol stocks for:

-I763028;

-pLac-cI-GFP;

-I763020;

-I763026.

  • Fluorescence test for:

-I763028;

-pLac-cI-GFP;

-I763028(midi).

  • Miniprep for:

-I763028;

-pLac-cI-GFP;

-I763026;

-I763020.

  • Digestion for:

-I763028 with Eco/Xba;

-I763026 with Eco/Spa;

-S0100 with Spa/Pst1;

-S0100 with Eco/Spa;

-I763020 with Xba/Pst1;

-pLac-cI-GFP with Xba/Pst1.

  • Band extraction from gel for all digestion except for pLac-cI-GFP. (photos)



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