Week 11

From 2007.igem.org

(Difference between revisions)
Line 39: Line 39:
* Digestion for:
* Digestion for:
-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;
 +
-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;
-[http://partsregistry.org/Part:BBa_J22101 J22101] with Xba/Pst1;

Revision as of 10:38, 12 September 2007

09/10/07
  • Miniprep for

-I763027;

-I763028;

-I763019;

-I763021+P0412;

-I763021+S03520;

-I763021+S0100;

Digestion for:

-I763027 with Xba/Spe;

-I763028 with Spe/Pst1 and with Xba/Spe;

-I763019 with Xba/Spe;

-I763021+P0412 with Eco/Spe;

-I763021+S03520 with Eco/Spe;

-I763021+S0100 with Eco/Spe;

  • Band extraction from gel for all digestion;
  • We have problems with J52034 and with I763019.
  • Ligations for:

-I763028 + I763007

-S0100 + J04431, because we want to understand if LacI operates well;

-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.

09/11/07
  • Digestion for:

-I763020 with Xba/Pst1;

-J22101 with Xba/Pst1;


09/12/07



09/13/07



09/14/07



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