Week 11
From 2007.igem.org
(Difference between revisions)
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* Control digestion before fluorescence tests. [[(photos)]] | * Control digestion before fluorescence tests. [[(photos)]] | ||
* Ligations for: | * Ligations for: | ||
- | -I7633005(Spe/Pst1) + | + | -I7633005(Spe/Pst1) + J04031(Xba/Pst1); |
+ | |||
+ | -S0100(Eco/Spe) + J04031(Eco/Xba); | ||
+ | |||
+ | - | ||
Revision as of 09:26, 20 September 2007
- 09/10/07
- Miniprep for
-I763027;
-I763028;
-I763019;
Digestion for:
-I763027 with Xba/Spe;
-I763028 with Spe/Pst1 and with Xba/Spe;
-I763019 with Xba/Spe;
- Band extraction from gel for all digestion;
- We have problems with J52034 and with I763019.
- Ligations for:
-S0100 + J04431, because we want to understand if LacI operates well;
-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.
- 09/11/07
- Digestion for:
-I763020 with Xba/Pst1;
-J22101 with Xba/Pst1;
- 09/12/07
- Control digestion before fluorescence tests. (photos)
- Ligations for:
-I7633005(Spe/Pst1) + J04031(Xba/Pst1);
-S0100(Eco/Spe) + J04031(Eco/Xba);
-
- 09/13/07
- 09/14/07