Week 11
From 2007.igem.org
- 09/10/07
- Miniprep for:
-I763027;
-I763028;
-I763019;
Digestion for:
-I763027 with Xba/Spe;
-I763028 with Spe/Pst1 and with Xba/Spe;
-I763019 with Xba/Spe;
- Band extraction from gel for all digestion;
- We have problems with J52034 and with I763019.
- Ligations for:
-S0100 + J04431, because we want to understand if LacI operates well;
-J06550 + J04631, with this ligation we want to understand if it operates well, because it doesn't leak.
- 09/11/07
- Digestion for:
-I763020 with Xba/Pst1;
-J22101 with Xba/Pst1;
- 09/12/07
- Control digestion before fluorescence tests. (photos)
- Ligations for:
-I7633005(Spe/Pst1) + J04031(Xba/Pst1);
-S0100(Eco/Spe) + J04031(Eco/Xba);
-I763015 (Eco/Spa) + J04031(Eco/Xba).
- 09/13/07
- We have find any colonies for I763015 + J04031;
- Miniprep for:
- I7633005 + J04031;
-S0100 + J04031;
-I763028.
- Control digestion for I763028.
- M9 Medium and lactose stocks preparation.
- 09/14/07
- Glicerol stocks for:
-I763028;
-pLac-cI-GFP;
-I763020;
-I763026.
- Fluorescence test for:
-I763028;
-pLac-cI-GFP;
-I763028(midi).
- Miniprep for:
-I763028;
-pLac-cI-GFP;
-I763026;
-I763020.
- Digestion for:
-I763028 with Eco/Xba;
-I763026 with Eco/Spa;
-S0100 with Spa/Pst1;
-S0100 with Eco/Spa;
-I763020 with Xba/Pst1;
-pLac-cI-GFP with Xba/Pst1.
- Band extraction from gel for all digestion except for pLac-cI-GFP. (photos)