http://2007.igem.org/wiki/index.php?title=Week_11&feed=atom&action=historyWeek 11 - Revision history2024-03-29T11:58:58ZRevision history for this page on the wikiMediaWiki 1.16.5http://2007.igem.org/wiki/index.php?title=Week_11&diff=42699&oldid=prevFrancesca.bugane2 at 15:53, 26 October 20072007-10-26T15:53:07Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Bologna | Back]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Bologna<ins class="diffchange diffchange-inline">#Diary </ins>| Back]]</div></td></tr>
</table>Francesca.bugane2http://2007.igem.org/wiki/index.php?title=Week_11&diff=23880&oldid=prevSilvia.tamarri2 at 12:24, 8 October 20072007-10-08T12:24:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763031 I763031] with Xba/Pst1.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion except for[http://partsregistry.org/Part:BBa_I763031 I763031]. [[(photos2)]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion except for [http://partsregistry.org/Part:BBa_I763031 I763031]. [[(photos2)]]</div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=23878&oldid=prevSilvia.tamarri2 at 12:23, 8 October 20072007-10-08T12:23:40Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763031;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">]</ins>;</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020];</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763031.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">]</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763031;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">]</ins>;</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763026 I763026];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763026 I763026];</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763031 with Xba/Pst1.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">] </ins>with Xba/Pst1.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion except for I763031. [[(photos2)]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion except for<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763031 </ins>I763031<ins class="diffchange diffchange-inline">]</ins>. [[(photos2)]]</div></td></tr>
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</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=23863&oldid=prevSilvia.tamarri2 at 11:02, 8 October 20072007-10-08T11:02:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">pLac-cI-GFP</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763031</ins>;</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020];</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">pLac-cI-GFP</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763031</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">pLac-cI-GFP</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763031</ins>;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763026 I763026];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763026 I763026];</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763020 I763020] with Xba/Pst1;</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">pLac-cI-GFP </del>with Xba/Pst1.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">I763031 </ins>with Xba/Pst1.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion except for <del class="diffchange diffchange-inline">pLac-cI-GFP</del>. [[(photos2)]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Band extraction from gel for all digestion except for <ins class="diffchange diffchange-inline">I763031</ins>. [[(photos2)]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=22406&oldid=prevSilvia.tamarri2 at 12:33, 3 October 20072007-10-03T12:33:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We take 5ml of LB medium in which we put a colony of bacteria which contain the [http://partsregistry.org/Part:BBa_I763028 I763028] plasmid.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We take 5ml of LB medium in which we put a colony of bacteria which contain the [http://partsregistry.org/Part:BBa_I763028 I763028] plasmid.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We examine a drop of it with our fluorescence microscopy. In these conditions, the bacteria <del class="diffchange diffchange-inline">doesn’t </del>beam fluorescence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We examine a drop of it with our fluorescence microscopy. In these conditions, the bacteria <ins class="diffchange diffchange-inline">don’t </ins>beam fluorescence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches 10mM, then 20mM and finally 40mM. Despite that, no fluorescence is visible.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches 10mM, then 20mM and finally 40mM. Despite that, no fluorescence is visible.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For this reason we then test single parts of the plasmid. We take three populations of [http://partsregistry.org/Part:BBa_I763019 I763019] bacteria and one of Plac-cI-GFP bacteria. We add 1mM of IPTG to every population to check for fluorescence. We see the three [http://partsregistry.org/Part:BBa_I763019 I763019] populations produce a photomultiplier output between 5.55 and 5.61Volts, while the Plac-cI-GFP population results more fluorescent giving a 5.91Volts output.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For this reason we then test single parts of the plasmid. We take three populations of [http://partsregistry.org/Part:BBa_I763019 I763019] bacteria and one of Plac-cI-GFP bacteria. We add 1mM of IPTG to every population to check for fluorescence. We see the three [http://partsregistry.org/Part:BBa_I763019 I763019] populations produce a photomultiplier output between 5.55 and 5.61Volts, while the Plac-cI-GFP population results more fluorescent giving a 5.91Volts output.</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=20035&oldid=prevSilvia.tamarri2 at 09:55, 21 September 20072007-09-21T09:55:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Testing our devices'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Testing our devices'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We take <del class="diffchange diffchange-inline">5 ml </del>of LB medium in which we put a colony of bacteria which contain the [http://partsregistry.org/Part:BBa_I763028 I763028] plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We take <ins class="diffchange diffchange-inline">5ml </ins>of LB medium in which we put a colony of bacteria which contain the [http://partsregistry.org/Part:BBa_I763028 I763028] plasmid.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We examine a drop of it <del class="diffchange diffchange-inline">using </del>our fluorescence microscopy <del class="diffchange diffchange-inline">setup</del>. In these conditions, the bacteria doesn’t beam fluorescence.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We examine a drop of it <ins class="diffchange diffchange-inline">with </ins>our fluorescence microscopy. In these conditions, the bacteria doesn’t beam fluorescence.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches <del class="diffchange diffchange-inline">10 mM</del>, then <del class="diffchange diffchange-inline">20 mM </del>and finally <del class="diffchange diffchange-inline">40 mM</del>. Despite that, no fluorescence is visible.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches <ins class="diffchange diffchange-inline">10mM</ins>, then <ins class="diffchange diffchange-inline">20mM </ins>and finally <ins class="diffchange diffchange-inline">40mM</ins>. Despite that, no fluorescence is visible.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For this reason we then test single parts of the plasmid. We take three populations of [http://partsregistry.org/Part:BBa_I763019 I763019] bacteria and one of Plac-cI-GFP bacteria. We add 1mM of IPTG to every population to check for fluorescence. We see the three [http://partsregistry.org/Part:BBa_I763019 I763019] populations produce a photomultiplier output between 5.55 and 5.<del class="diffchange diffchange-inline">61 Volts</del>, while the Plac-cI-GFP population results more fluorescent giving a 5.<del class="diffchange diffchange-inline">91 Volts </del>output.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For this reason we then test single parts of the plasmid. We take three populations of [http://partsregistry.org/Part:BBa_I763019 I763019] bacteria and one of Plac-cI-GFP bacteria. We add 1mM of IPTG to every population to check for fluorescence. We see the three [http://partsregistry.org/Part:BBa_I763019 I763019] populations produce a photomultiplier output between 5.55 and 5.<ins class="diffchange diffchange-inline">61Volts</ins>, while the Plac-cI-GFP population results more fluorescent giving a 5.<ins class="diffchange diffchange-inline">91Volts </ins>output.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Every time we check for fluorescence, we use, as said, not only the photo camera but also the photomultiplier. Every measurement shows a 0.<del class="diffchange diffchange-inline">18 Volts </del>offset due to environment light and a 1.<del class="diffchange diffchange-inline">30 Volts </del>offset due to the excitation light at <del class="diffchange diffchange-inline">501 nm</del>. Since we know GFP gives a maximum of fluorescence when excited at <del class="diffchange diffchange-inline">501 nm</del>, we decide to go on with this wavelength despite the offset (a change of excitation wavelength reduces both the offset and the signal).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Every time we check for fluorescence, we use, as said, not only the photo camera but also the photomultiplier. Every measurement shows a 0.<ins class="diffchange diffchange-inline">18Volts </ins>offset due to environment light and a 1.<ins class="diffchange diffchange-inline">30Volts </ins>offset due to the excitation light at <ins class="diffchange diffchange-inline">501nm</ins>. Since we know GFP gives a maximum of fluorescence when excited at <ins class="diffchange diffchange-inline">501nm</ins>, we decide to go on with this wavelength despite the offset (a change of excitation wavelength reduces both the offset and the signal).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=19841&oldid=prevSilvia.tamarri2 at 13:50, 20 September 20072007-09-20T13:50:05Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:50, 20 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 68:</td>
<td colspan="2" class="diff-lineno">Line 68:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Testing our devices'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Testing our devices'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We take 5 ml of LB medium in which we put a colony of bacteria which contain the[http://partsregistry.org/Part:BBa_I763028 I763028] plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We take 5 ml of LB medium in which we put a colony of bacteria which contain the <ins class="diffchange diffchange-inline"> </ins>[http://partsregistry.org/Part:BBa_I763028 I763028] plasmid.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We examine a drop of it using our fluorescence microscopy setup. In these conditions, the bacteria doesn’t beam fluorescence.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We examine a drop of it using our fluorescence microscopy setup. In these conditions, the bacteria doesn’t beam fluorescence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches 10 mM, then 20 mM and finally 40 mM. Despite that, no fluorescence is visible.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches 10 mM, then 20 mM and finally 40 mM. Despite that, no fluorescence is visible.</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=19840&oldid=prevSilvia.tamarri2 at 13:49, 20 September 20072007-09-20T13:49:24Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:49, 20 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 90:</td>
<td colspan="2" class="diff-lineno">Line 90:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-pLac-cI-GFP<del class="diffchange diffchange-inline">;</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-pLac-cI-GFP<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">-[http://partsregistry.org/Part:BBa_I763019 I763019](midi).</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-[http://partsregistry.org/Part:BBa_I763019 I763019];</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=19839&oldid=prevSilvia.tamarri2 at 13:48, 20 September 20072007-09-20T13:48:29Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:48, 20 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 68:</td>
<td colspan="2" class="diff-lineno">Line 68:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Testing our devices'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''Testing our devices'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We take 5 ml of LB medium in which we put a colony of bacteria which contain the <del class="diffchange diffchange-inline">Ptet-LacI Plac-cI-LacY-GFP </del>plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We take 5 ml of LB medium in which we put a colony of bacteria which contain the<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763028 I763028] </ins>plasmid.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We examine a drop of it using our fluorescence microscopy setup. In these conditions, the bacteria doesn’t beam fluorescence.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We examine a drop of it using our fluorescence microscopy setup. In these conditions, the bacteria doesn’t beam fluorescence.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches 10 mM, then 20 mM and finally 40 mM. Despite that, no fluorescence is visible.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Starting from this moment, we add into the LB medium 1mM of IPTG every 30 minutes until it reaches 4mM , checking each time for any possible fluorescence, with negative results. We then try another option: after diluting 1ml of the original solution with 4ml of LB, we add IPTG until this solution reaches 10 mM, then 20 mM and finally 40 mM. Despite that, no fluorescence is visible.</div></td></tr>
</table>Silvia.tamarri2http://2007.igem.org/wiki/index.php?title=Week_11&diff=19836&oldid=prevSilvia.tamarri2 at 13:43, 20 September 20072007-09-20T13:43:19Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:43, 20 September 2007</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 92:</td>
<td colspan="2" class="diff-lineno">Line 92:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-pLac-cI-GFP;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-pLac-cI-GFP;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">I763028</del>(midi).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763019 I763019]</ins>(midi).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep for:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">I763028</del>;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763019 I763019]</ins>;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-pLac-cI-GFP;</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-pLac-cI-GFP;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763026;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763026 </ins>I763026<ins class="diffchange diffchange-inline">]</ins>;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763020.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763020 </ins>I763020<ins class="diffchange diffchange-inline">]</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion for:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Digestion for:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">I763028 </del>with Eco/Xba;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763019 I763019] </ins>with Eco/Xba;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763026 with Eco/Spe;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763026 </ins>I763026<ins class="diffchange diffchange-inline">] </ins>with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">S0100 </del>with Spe/Pst1;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763026 I763026] </ins>with Spe/Pst1;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-<del class="diffchange diffchange-inline">S0100 </del>with Eco/Spe;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763026 I763026] </ins>with Eco/Spe;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-I763020 with Xba/Pst1;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_I763020 </ins>I763020<ins class="diffchange diffchange-inline">] </ins>with Xba/Pst1;</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-pLac-cI-GFP with Xba/Pst1.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-pLac-cI-GFP with Xba/Pst1.</div></td></tr>
</table>Silvia.tamarri2