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Line 14:		Line 14:
	-J04431;		-J04431;
-
-	-Ptet-LacI-GFP(Eco/Spe), (Eco/Xba);
	-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).		-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).
Line 25:		Line 23:
	-Ptet-LacI-GFP with Eco/Spe;		-Ptet-LacI-GFP with Eco/Spe;
		+	-I763007 with Eco/Xba;
-	-Ptet-LacI-GFP with Eco/Spe;	+	-Plac-cI-LacY with Eco/Spe1;
-	-I763007 with Eco/Xba;	+	*Band extraction from gel for all digestion and then we observe:
		+	-I763027, J04431 are died;
-	-Plac-cI-LAcY with Eco/Spe1;	+	-Ptet-LacI-GFP is correct;
-	*Band extraction from gel for all digestion and then we observe:	+	-I763007 not found;
-	-I763027, J04431,    are died;	+
-	-	+	-Plac-cI-LacY is correct.

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Revision as of 12:49, 20 September 2007

09/17/07

09/18/07

09/19/07

• Miniprep for:

-I763027;

-J04431;

-Ptet-LacI-GFP(Spe/Pst1), (Xba/Pst1).

• Digestion for:

-I763027 with Eco/Spe;

-J04431 with Eco/Spe;

-Ptet-LacI-GFP with Eco/Spe;

-I763007 with Eco/Xba;

-Plac-cI-LacY with Eco/Spe1;

• Band extraction from gel for all digestion and then we observe:

-I763027, J04431 are died;

-Ptet-LacI-GFP is correct;

-I763007 not found;

-Plac-cI-LacY is correct.

09/20/07

09/21/07

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